Výsledky vyhledávání - "Francois-Yves bouget"

Transient transformation of Ostreococcus species (OTTH595, RCC809 and RCC802) and Bathycoccus v3

Autor: Francois Yves Bouget, Francois-Yves Bouget

This protocol describes the preparation of cells and introduction of DNA into the cells by electroporation. For selection of stable transformants or measure of transient gene expression see related protocols.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cae4195e20aaf925b682a2727ff4871d
https://doi.org/10.17504/protocols.io.83uhynw

Zobrazit plný text záznamu

Selection of stable transformants in Ostreococcus tauri, Ostreococcus lucimarinus and Bathycoccus prasinos v3

Autor: Francois-Yves bouget

This protocol describes the selection and growth of stable transformants in semi solid agarose medium. Developped initially for Ostreococcus tauri, it also works for Ostreococcus lucimarinus and Bathycoccus.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::72d55d3b35afb5a70ae5062e1a5b0391
https://doi.org/10.17504/protocols.io.zj2f4qe

Zobrazit plný text záznamu

Selection of stable transformants in Ostreococcus tauri and Bathycoccus prasinos v2

Autor: Francois-Yves bouget

This protocol describes the selection and growth of stable transformants in semi solid agarose medium. Developped initially for Ostreococcus tauri, it also works for Bathycoccus.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3e7e8831baef65b72b2b58b30c529fb3
https://doi.org/10.17504/protocols.io.hddb226

Zobrazit plný text záznamu

Transient luciferase expression in Ostreococcus (OTTH595, RCC809) and Bathycoccus v1

Autor: Francois-Yves Bouget, not provided Valérie Vergé et Jean-Claude Lozano

This protocol describes the quantification of luciferase activity in Ostreococcus and Bathycoccus cells that have been transformed with a firefly luciferase coding sequence upstream of the OTTH595 high affinity transporter promoter. Using this method

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d21a97946b3f211f9d8de0a9617ffa82
https://doi.org/10.17504/protocols.io.hcib2ue

Zobrazit plný text záznamu