Výsledky vyhledávání - "Francis P. Barbone"

Mutagenesis Studies of the Human Erythropoietin Receptor

Autor: Jennifer Tullai, Linda K. Jolliffe, Adriane E. Schilling, Steven A. Middleton, Mulcahy Linda, Dana L. Johnson, Francis P. Barbone, Frank J. McMahon, Robert H. Gruninger

Publikováno v: Journal of Biological Chemistry. 272:4985-4992

Mutagenesis of the erythropoietin receptor (EPOR) permits analysis of the contribution that individual amino acid residues make to erythropoietin (EPO) binding. We employed both random and site-specific mutagenesis to determine the function of amino

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c16cac2dc33f590bcd921b7a40564b55
https://doi.org/10.1074/jbc.272.8.4985

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Small Peptides as Potent Mimetics of the Protein Hormone Erythropoietin

Autor: Dana L. Johnson, Francis P. Barbone, William J. Dower, Ronald W. Barrett, Francis X. Farrell, Arun K. Kashyap, Linda K. Jolliffe, Mulcahy Linda, Chang Ray S, Wrighton Nicholas C

Publikováno v: Science. 273:458-463

Random phage display peptide libraries and affinity selective methods were used to isolate small peptides that bind to and activate the receptor for the cytokine erythropoietin (EPO). In a panel of in vitro biological assays, the peptides act as full

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7df2d28b9b947e40bb94246b48032a7d
https://doi.org/10.1126/science.273.5274.458

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Yeast killer virus transcription initiation in vitro

Autor: Michael J. Leibowitz, Teresa L. Williams, Francis P. Barbone

Publikováno v: Virology. 187:333-337

Killer virions isolated from infected Saccharomyces cerevisiae cells contain an RNA polymerase activity which catalyzes the transcription in vitro of positive polarity RNAs from the L-A and M double-stranded RNA genomic segments of the virus. The RNA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70f50e99ada3fd79435779f54441727a
https://doi.org/10.1016/0042-6822(92)90323-h

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Coupling of killer virus transcription with translation in yeast cell-free extracts

Autor: Michael J. Leibowitz, Francis P. Barbone

Publikováno v: Journal of General Virology. 72:1755-1760

The cytoplasmically inherited killer virus of Saccharomyces cerevisiae expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be adde

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43d4303d4b937c4d9c8734ccc5e37ea2
https://doi.org/10.1099/0022-1317-72-7-1755

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An antagonist peptide-EPO receptor complex suggests that receptor dimerization is not sufficient for activation

Autor: Sidney Pestka, Oded Livnah, Francis X. Farrell, Mark A. Goldsmith, Yun You, Enrico A. Stura, Wen He, Ian A. Wilson, Christopher D. Krause, Dana L. Johnson, Francis P. Barbone, Kathleen D. Liu, Linda K. Jolliffe

Publikováno v: Nature structural biology. 5(11)

Dimerization of the erythropoietin (EPO) receptor (EPOR), in the presence of either natural (EPO) or synthetic (EPO-mimetic peptides, EMPs) ligands is the principal extracellular event that leads to receptor activation. The crystal structure of the e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5d2d6449b142d02db6e1cb5a1f0df5c
https://pubmed.ncbi.nlm.nih.gov/9808034

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Amino-terminal dimerization of an erythropoietin mimetic peptide results in increased erythropoietic activity

Autor: Frank J. McMahon, Mulcahy Linda, Francis X. Farrell, James Freedy, Robert Allan Zivin, Dana L. Johnson, Francis P. Barbone, Jennifer Tullai, Linda K. Jolliffe, Daniel J. Kroon

Publikováno v: Chemistrybiology. 4(12)

Background: Erythropoietin (EPO), the hormone involved in red blood cell production, activates its receptor by binding to the receptor's extracellular domain and presumably dimerizing two receptor monomers to initiate signal transduction. EPO-mimetic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a367a550721c9353f91be54b6211541
https://pubmed.ncbi.nlm.nih.gov/9427659

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Increased potency of an erythropoietin peptide mimetic through covalent dimerization

Autor: Ronald W. Barrett, Palaniappan Balasubramanian, Wrighton Nicholas C, William J. Dower, Francis X. Farrell, Arun K. Kashyap, Linda K. Jolliffe, Francis P. Barbone

Publikováno v: Nature biotechnology. 15(12)

We have synthesized a chemically defined, dimeric form of an erythropoietin mimetic peptide (EMP) that displays 100-fold increased affinity for the erythropoietin receptor (EPOR) and correspondingly elevated potency in cell-based assays and in mice.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0486dc467ccda9ec0a64e9c522493155
https://pubmed.ncbi.nlm.nih.gov/9359108

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Refolding, purification, and characterization of human erythropoietin binding protein produced in Escherichia coli

Autor: Frank J. McMahon, Woan Hwa Lee, Dana L. Johnson, Francis P. Barbone, Eric Tsao, Steven A. Middleton, Linda K. Jolliffe, Daniel Kroon, Mulcahy Linda

Publikováno v: Protein expression and purification. 7(1)

The extracellular domain of the human erythropoietin receptor (EPO binding protein (EBP)) has been expressed and overproduced in Escherichia coli. Regardless of the presence of pelB or ompT signal sequences the recombinant protein produced in this fa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d94b8a0303920f675af4463391f59df
https://pubmed.ncbi.nlm.nih.gov/9172774

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