Zobrazeno 1 - 10
of 74
pro vyhledávání: '"Francis E Nano"'
Publikováno v:
PLoS ONE, Vol 9, Iss 8, p e104881 (2014)
The highly infectious bacteria, Francisella tularensis, colonize a variety of organs and replicate within both phagocytic as well as non-phagocytic cells, to cause the disease tularemia. These microbes contain a conserved cluster of important virulen
Externí odkaz:
https://doaj.org/article/dc8103ef7f7a4f5b85d184cb61b3d600
Autor:
Mia D Champion, Qiandong Zeng, Eli B Nix, Francis E Nano, Paul Keim, Chinnappa D Kodira, Mark Borowsky, Sarah Young, Michael Koehrsen, Reinhard Engels, Matthew Pearson, Clint Howarth, Lisa Larson, Jared White, Lucia Alvarado, Mats Forsman, Scott W Bearden, Anders Sjöstedt, Richard Titball, Stephen L Michell, Bruce Birren, James Galagan
Publikováno v:
PLoS Pathogens, Vol 5, Iss 5, p e1000459 (2009)
Tularemia is a geographically widespread, severely debilitating, and occasionally lethal disease in humans. It is caused by infection by a gram-negative bacterium, Francisella tularensis. In order to better understand its potency as an etiological ag
Externí odkaz:
https://doaj.org/article/52a48aebe0a644238f82d11778671b61
Autor:
Bailey E. McGuire, Francis E. Nano
Publikováno v:
BMC Genomics, Vol 24, Iss 1, Pp 1-19 (2023)
Abstract Background High temperatures cause a suite of problems for cells, including protein unfolding and aggregation; increased membrane fluidity; and changes in DNA supercoiling, RNA stability, transcription and translation. Consequently, enhanced
Externí odkaz:
https://doaj.org/article/b449691375f24291a69f8418441b73c9
Autor:
Dirk F. H. Winkler, Julia E Mela, Steven L. Pelech, Vanessa C Thompson, Bailey E. McGuire, Logan R Cucsksey, Ralph L McWhinnie, Claire E Stevens, Francis E. Nano
Publikováno v:
Microbial Cell Factories, Vol 21, Iss 1, Pp 1-13 (2022)
Microbial Cell Factories
Microbial Cell Factories
We have developed a method for the inexpensive, high-level expression of antigenic protein fragments of SARS-CoV-2 proteins in Escherichia coli. Our approach uses the thermophilic family 9 carbohydrate-binding module (CBM9) as an N-terminal carrier p
Publikováno v:
FEMS Microbiology Letters. 370
In order to facilitate Golden Gate DNA assembly, we have constructed a collection of Bacillus subtilis replicative plasmids representing five origins of replication derived from plasmids pUB110, pE194, pWV01, pBS72, and pTH1030. The first three of th
Autor:
Sheila Potter, Mia S Frier, Tyler W Dyer, Max S G Legg, Geoff Gudavicius, Francis E. Nano, Vanessa C Thompson, Bailey E. McGuire
Publikováno v:
FEMS microbiology letters. 367(19)
We used error-prone PCR to generate mutations in a subtilisin protease-encoding gene, and screened for recombinants that expressed temperature-sensitive (TS) variants. From the dozens of mutations that we detected in the recombinant genes we found th
Publikováno v:
Journal of microbiology, biotechnology and food sciences. 7:145-148
Food preservation is a vital issue on a global scale. It reflects both the need to prevent food spoilage as well as the necessity for reduction in the occurrence of food-borne pathogens. Progress of technology allowed for the development of various t
Publikováno v:
Microbiology. 163:462-471
Temperature sensitivity is often used as a way to attenuate micro-organisms to convert them into live vaccines. In this work, we explore the use of temperature-sensitive (TS) genetic circuits that express lethal genes as a widely applicable approach
Autor:
Angela Hartman, Stephanie C. Pearce, Francis E. Nano, Keith A. Ameiss, Everett L. Rosey, Stephanie M. Puckett, Barry N. Duplantis
Publikováno v:
Applied and Environmental Microbiology. 81:6757-6766
Synthetic genes based on deduced amino acid sequences of the NAD-dependent DNA ligase ( ligA ) and CTP synthetase ( pyrG ) of psychrophilic bacteria were substituted for their native homologues in the genome of Salmonella enterica serovar Enteritidis
Autor:
Jan-Hendrik Hehemann, Dusko Posarac, Heli Wang, Swati Yewalkar, Francis E. Nano, Xiaotao Bi, Layne A. Woodfin, Sheldon J.B. Duff, Di Li, Sheila Potter
Publikováno v:
Frontiers of Environmental Science & Engineering. 11
The gene for the catalytic domain of thermostable endo-β-1,3-glucanase (laminarinase) LamA was cloned from Thermotoga maritima MSB8 and heterologously expressed in a bioengineered Synechococcus sp. PCC 7002. The mutant strain was cultured in a photo