A comparison of machine learning approaches for the quantification of microglial cells in the brain of mice, rats and non-human primates.

Autor: Danish M Anwer, Francesco Gubinelli, Yunus A Kurt, Livija Sarauskyte, Febe Jacobs, Chiara Venuti, Ivette M Sandoval, Yiyi Yang, Jennifer Stancati, Martina Mazzocchi, Edoardo Brandi, Gerard O'Keeffe, Kathy Steece-Collier, Jia-Yi Li, Tomas Deierborg, Fredric P Manfredsson, Marcus Davidsson, Andreas Heuer

Publikováno v: PLoS ONE, Vol 18, Iss 5, p e0284480 (2023)

Microglial cells are brain-specific macrophages that swiftly react to disruptive events in the brain. Microglial activation leads to specific modifications, including proliferation, morphological changes, migration to the site of insult, and changes

Externí odkaz: https://doaj.org/article/fcdfb7538fd1416cbc6c38dd6c8d0c4e

The C-terminal domain of LRRK2 with the G2019S mutation is sufficient to produce neurodegeneration of dopaminergic neurons in vivo

Autor: Noémie Cresto, Marie-Claude Gaillard, Camille Gardier, Francesco Gubinelli, Elsa Diguet, Déborah Bellet, Laurine Legroux, Julien Mitja, Gwenaëlle Auregan, Martine Guillermier, Charlène Josephine, Caroline Jan, Noëlle Dufour, Alain Joliot, Philippe Hantraye, Gilles Bonvento, Nicole Déglon, Alexis-Pierre Bemelmans, Karine Cambon, Géraldine Liot, Emmanuel Brouillet

Publikováno v: Neurobiology of Disease, Vol 134, Iss , Pp - (2020)

The G2019S substitution in the kinase domain of LRRK2 (LRRK2G2019S) is the most prevalent mutation associated with Parkinson's disease (PD). Neurotoxic effects of LRRK2G2019S are thought to result from an increase in its kinase activity as compared t

Externí odkaz: https://doaj.org/article/f99d8776c12c4a7bb6880f866319890e

Sequential or simultaneous injection of preformed fibrils and AAV overexpression of alpha-synuclein are equipotent in producing relevant pathology and behavioral deficits

Autor: Matilde Negrini, Giuseppe Tomasello, Marcus Davidsson, Alexis Fenyi, Cécile Adant, Swantje Hauser, Elena Espa, Francesco Gubinelli, Fredric P. Manfredsson, Ronald Melki, Andreas Heuer

Publikováno v: Journal of Parkinson's disease
Journal of Parkinson's disease, 2022, 12, pp.1133-1153. ⟨10.3233/jpd-212555⟩

Background: Preclinical rodent models for Parkinson’s disease (PD) based on viral human alpha-synuclein (h-αSyn) overexpression recapitulate some of the pathological hallmarks as it presents in humans, such as progressive cell loss and additional

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a5627c4f9475b79e30a91caae6c7412
https://cea.hal.science/cea-03683745/document

The influence of the closure format on the storage stability and moisture content of freeze-dried influenza antigen

Autor: Francesco Gubinelli, Sitara Dubey, Arnold Duralliu, Daryl R. Williams, Paul Matejtschuk, Halima Koroma

Publikováno v: Vaccine. 37:4485-4490

Low moisture content is seen as crucial to achieving long term stability of freeze dried biologics and reference materials. Highly hygroscopic freeze-dried material are susceptible to moisture ingress over time which can lead to degradation and loss

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4deef4cdf7a7d05e537f359822af553
https://doi.org/10.1016/j.vaccine.2019.06.070

The C-Terminal Domain of LRRK2 with the G2019S Substitution Increases Mutant A53T α-Synuclein Toxicity in Dopaminergic Neurons In Vivo

Autor: Sueva Bernier, Alexis-Pierre Bemelmans, Noelle Dufour, Gilles Bonvento, Marie-Claude Gaillard, Géraldine Liot, Pauline Gipchtein, Noémie Cresto, Camille Gardier, Philippe Hantraye, Gwenaëlle Auregan, Nadja Van Camp, Daniela Molina, Caroline Jan, Martine Guillermier, Marie-Christine Chartier-Harlin, Francesco Gubinelli, Jean-Marc Taymans, Pauline Roost, Charlène Joséphine, Karine Cambon, Emmanuel Brouillet

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences, 2021, 22 (13), pp.6760. ⟨10.3390/ijms22136760⟩
Volume 22
Issue 13
International Journal of Molecular Sciences, Vol 22, Iss 6760, p 6760 (2021)
International Journal of Molecular Sciences, MDPI, 2021, 22 (13), pp.6760. ⟨10.3390/ijms22136760⟩

Alpha-synuclein (α-syn) and leucine-rich repeat kinase 2 (LRRK2) play crucial roles in Parkinson’s disease (PD). They may functionally interact to induce the degeneration of dopaminergic (DA) neurons via mechanisms that are not yet fully understoo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20e4f14cf397c9aa3340a960434da9ca
https://hal.science/hal-03452142