Thermal stabilization of the deglycating enzyme Amadoriase I by rational design

Autor: Federica Rigoldi, Stefano Donini, Francesca Giacomina, Federico Sorana, Alberto Redaelli, Tiziano Bandiera, Emilio Parisini, Alfonso Gautieri

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)

Abstract Amadoriases are a class of FAD-dependent enzymes that are found in fungi, yeast and bacteria and that are able to hydrolyze glycated amino acids, cleaving the sugar moiety from the amino acidic portion. So far, engineered Amadoriases have mo

Externí odkaz: https://doaj.org/article/6eefa2ae873945d49454695efac1e953

Identification, Structure–Activity Relationship, and Biological Characterization of 2,3,4,5-Tetrahydro-1H-pyrido[4,3-b]indoles as a Novel Class of CFTR Potentiators

Autor: Federico Sorana, Rosalia Bertorelli, Simone Giovani, Tiziano Bandiera, Giuliana Ottonello, Sine Mandrup Bertozzi, Francesca Giacomina, Emanuela Pesce, Luca Goldoni, Ambra Gianotti, Loretta Ferrera, Paolo Di Fruscia, Nicoletta Brindani, Elvira Sondo, Nicoletta Pedemonte, Debora Russo, Ilaria Penna, Luis J. V. Galietta, Maria Summa, Fabio Bertozzi

Publikováno v: Journal of Medicinal Chemistry

Cystic fibrosis (CF) is a life-threatening autosomal recessive disease, caused by mutations in the CF transmembrane conductance regulator (CFTR) chloride channel. CFTR modulators have been reported to address the basic defects associated with CF-caus

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39653fbd8f273d3e0d754f0023b651f3
https://doi.org/10.1021/acs.jmedchem.0c01050

Fluorine nuclear magnetic resonance-based assay in living mammalian cells

Autor: Daniele Piomelli, Claudio Dalvit, Tiziano Bandiera, Beatrice Castellani, Elisa Romeo, Chiara Lambruschini, Rita Scarpelli, Giuliana Ottonello, Gianpiero Garau, Francesca Giacomina, Marina Veronesi

Publikováno v: Veronesi, M; Giacomina, F; Romeo, E; Castellani, B; Ottonello, G; Lambruschini, C; et al.(2016). Fluorine nuclear magnetic resonance-based assay in living mammalian cells. Analytical Biochemistry, 495, 52-59. doi: 10.1016/j.ab.2015.11.015. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/31j1k53g

© 2015 Elsevier Inc. All rights reserved. Nuclear magnetic resonance (NMR)-based screening has been recognized as a powerful approach for the identification and characterization of molecules interacting with pharmaceutical targets. Indeed, several N

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ee82d2fd22dbc839edcbe1714c3e4eb
http://hdl.handle.net/11567/937783

ChemInform Abstract: Construction of Enantioenriched Cyclic Compounds by Asymmetric Allylic Alkylation and Ring-Closing Metathesis

Autor: Alexandre Alexakis, Francesca Giacomina

Publikováno v: ChemInform. 45

In some cases the two step procedure is performed in one pot without quenching the reaction mixture after the allylic alkylation step before the Grubbs catalyst is added.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c75d94998e84d5795b496bf9a1880883
https://doi.org/10.1002/chin.201410057

Thermal stabilization of the deglycating enzyme Amadoriase I by rational design

Autor: Tiziano Bandiera, Federico Sorana, Stefano Donini, Alfonso Gautieri, Emilio Parisini, Alberto Redaelli, Francesca Giacomina, Federica Rigoldi

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
Scientific Reports

Amadoriases are a class of FAD-dependent enzymes that are found in fungi, yeast and bacteria and that are able to hydrolyze glycated amino acids, cleaving the sugar moiety from the amino acidic portion. So far, engineered Amadoriases have mostly foun

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::517735d898593e3dce1d5a88fb4ff5bb