Restoring the biological activity of crizanlizumab at physiological conditions through a pH-dependent aspartic acid isomerization reaction

Autor: Fabian Bickel, François Griaud, Wolfram Kern, Frieder Kroener, Manuela Gritsch, Jérôme Dayer, Samuel Barteau, Blandine Denefeld, Chi-Ya Kao-Scharf, Manuel Lang, Izabela Slupska-Muanza, Carla Schmidt, Matthias Berg, Jürgen Sigg, Lina Boado, Dirk Chelius

Publikováno v: mAbs, Vol 15, Iss 1 (2023)

ABSTRACTIn this study, we report the isomerization of an aspartic acid residue in the complementarity-determining region (CDR) of crizanlizumab as a major degradation pathway. The succinimide intermediate and iso-aspartic acid degradation products we

Externí odkaz: https://doaj.org/article/704ad1576cf945d7aab734d00191b085

All Ion Differential Analysis Refines the Detection of Terminal and Internal Diagnostic Fragment Ions for the Characterization of Biologics Product-Related Variants and Impurities by Middle-down Mass Spectrometry

Autor: Manuel Lang, Jian-You Chen, Blandine Denefeld, Chi-Ya Kao-Scharf, Matthias Berg, François Griaud, Jerôme Dayer

Publikováno v: Analytical chemistry. 91(14)

Characterization and monitoring of post-translational modifications (PTMs) are key analytical requirements during the development of biologics. Top and middle-down (MD) approaches aim at capturing a direct snapshot of all proteoforms with their combi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9996c56517528e8e1269c0b5d50c557
https://pubmed.ncbi.nlm.nih.gov/31187983

Unbiased in-depth characterization of CEX fractions from a stressed monoclonal antibody by mass spectrometry

Autor: Peter Haberl, Matthias Berg, François Griaud, Blandine Denefeld, Manuel Lang, Héloïse Hensinger

Publikováno v: mAbs. 9(5)

Characterization of charge-based variants by mass spectrometry (MS) is required for the analytical development of a new biologic entity and its marketing approval by health authorities. However, standard peak-based data analysis approaches are time-c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e447b748a0e0f41083d93f4efbacf0e7
https://pubmed.ncbi.nlm.nih.gov/28379786

BCR/ABL modulates protein phosphorylation associated with the etoposide-induced DNA damage response

Autor: Anthony D. Whetton, Andrew Pierce, Andrew J.K. Williamson, David N. Potier, Samuel J. Taylor, François Griaud, Elaine Spooncer

Publikováno v: Griaud, F, Williamson, A J K, Taylor, S, Potier, D N, Spooncer, E, Pierce, A & Whetton, A D 2012, ' BCR/ABL modulates protein phosphorylation associated with the etoposide-induced DNA damage response ', Journal of Proteomics, vol. 77, pp. 14-26 . https://doi.org/10.1016/j.jprot.2012.06.003

BCR/ABL expression is the key characteristic of chronic myeloid leukaemia (CML). The progression of CML is associated with genomic instability. Systematic analysis of DNA damage signalling in the presence of BCR/ABL thus offers opportunities to ident

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ceb7b19d4216eac72c01f198e33ade72
https://pubmed.ncbi.nlm.nih.gov/22705319