Zobrazeno 1 - 8
of 8
pro vyhledávání: '"François Bouthillier"'
Autor:
Alena Kuderova, Jeremy S. Lee, Phani Raj Pokkuluri, Yunge Li, Miroslaw Cygler, François Bouthillier
Publikováno v:
Journal of Molecular Biology. 243:283-297
Fab fragments from Jel 103, an antibody which specifically binds to single-stranded poly(rl), were prepared by papain digestion, separated into eight isoforms and characterized by mass spectrometry. One of the purified isoforms yielded crystals suita
Publikováno v:
Journal of Steroid Biochemistry. 33:993-1000
Rat prostate cytosolic androgen-receptor complexes were analyzed by sucrose density gradient (SDG) centrifugation and by high-performance liquid chromatofocusing (HPCF). Without protecting agents, these complexes were resolved by HPCF at basic (8.25
Application of high-performance liquid chromatofocusing to the study of prostatic androgen receptors
Publikováno v:
Journal of Chromatography A. 403:171-182
Cytosolic androgen receptors from normal rat prostate were analyzed by high-performance liquid chromatofocusing. Two ion-exchange columns, AX-300 and AX-500, and two equilibration systems, Tris-HCl and imidazole-HCl, were used. pH gradients ranged be
Publikováno v:
Journal of steroid biochemistry. 30(1-6)
We have studied the properties of the nuclear receptors for aldosterone in kidneys of chick embryo. Aliquots of 0.4 M KO nuclear extracts were incubated with [ 3 H]aldosterone with or without 1 μM RU28362, a potent glucocorticoid analog. Scatchard a
Publikováno v:
Journal of steroid biochemistry. 30(1-6)
The isolation and characterization of the untransformed form of androgen receptors has not yet been successful, owing to their inherent lability as well as to their ready proteolysis. In this study, we have stabilized rat prostate androgen receptors
Autor:
Farouk Radwan, Michel Carmel, Mostafa M. Elhilali, Francine Grondin, Michael Koutsilieris, Jean-Guy Lehoux, François Bouthillier
Publikováno v:
The Prostate. 13(2)
We investigated the application of octadecylsilyl (ODS)-silica in studies related to characterization and purification of the nontransformed rat ventral prostate androgen receptor. The results indicated that ODS-silica successfully separates the free
Publikováno v:
Molecular and Cellular Biochemistry. 90
In the presence of sodium molybdate and protease inhibitors, two forms of androgen-receptor complexes were observed which sedimented in the areas of 8–9S and 5–7S by SDG centrifugation. The intermediary 5–7S form was better seen when complexes
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