NMR study of human macroPARPs domains: ¹H, ¹⁵N and ¹³C resonance assignment of hPARP14 macro domain 2 in the free and the ADPr bound state.

Autor: Fourkiotis, Nikolaos K., Charalampous, Periklis, Tsika, Aikaterini C., Kravvariti, Konstantina P., Sideras-Bisdekis, Christos, Gallo, Angelo, Spyroulias, Georgios A.

Publikováno v: Biomolecular NMR Assignments; Oct2022, Vol. 16 Issue 2, p399-406, 8p

1H,13C and 15N chemical shift assignments of the SUD domains of SARS-CoV-2 non-structural protein 3c: "the N-terminal domain-SUD-N".

Autor: Gallo, Angelo, Tsika, Aikaterini C., Fourkiotis, Nikolaos K., Cantini, Francesca, Banci, Lucia, Sreeramulu, Sridhar, Schwalbe, Harald, Spyroulias, Georgios A.

Publikováno v: Biomolecular NMR Assignments; Apr2021, Vol. 15 Issue 1, p85-89, 5p

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Autor: Altincekic, Nadide, Korn, Sophie Marianne, Qureshi, Nusrat Shahin, Dujardin, Marie, Ninot-Pedrosa, Martí, Abele, Rupert, Abi Saad, Marie Jose, Alfano, Caterina, Almeida, Fabio C. L., Alshamleh, Islam, De Amorim, Gisele Cardoso, Anderson, Thomas K., Anobom, Cristiane D., Anorma, Chelsea, Bains, Jasleen Kaur, Bax, Adriaan, Blackledge, Martin, Blechar, Julius, Böckmann, Anja, Brigandat, Louis, Bula, Anna, Bütikofer, Matthias, Camacho-Zarco, Aldo R., Carlomagno, Teresa, Caruso, Icaro Putinhon, Ceylan, Betül, Chaikuad, Apirat, Chu, Feixia, Cole, Laura, Crosby, Marquise G., De Jesus, Vanessa, Dhamotharan, Karthikeyan, Felli, Isabella C., Ferner, Jan, Fleischmann, Yanick, Fogeron, Marie-Laure, Fourkiotis, Nikolaos K., Fuks, Christin, Fürtig, Boris, Gallo, Angelo, Gande, Santosh L., Gerez, Juan Atilio, Ghosh, Dhiman, Gomes-Neto, Francisco, Gorbatyuk, Oksana, Guseva, Serafima, Hacker, Carolin, Häfner, Sabine, Hao, Bing, Hargittay, Bruno, Henzler-Wildman, K., Hoch, Jeffrey C., Hohmann, Katharina F., Hutchison, Marie T., Jaudzems, Kristaps, Jović, Katarina, Kaderli, Janina, Kalniņš, Gints, Kaņepe, Iveta, Kirchdoerfer, Robert N., Kirkpatrick, John, Knapp, Stefan, Krishnathas, Robin, Kutz, Felicitas, Zur Lage, Susanne, Lambertz, Roderick, Lang, Andras, Laurents, Douglas, Lecoq, Lauriane, Linhard, Verena, Löhr, Frank, Malki, Anas, Bessa, Luiza Mamigonian, Martin, Rachel W., Matzel, Tobias, Maurin, Damien, McNutt, Seth W., Mebus-Antunes, Nathane Cunha, Meier, Beat H., Meiser, Nathalie, Mompeán, Miguel, Monaca, Elisa, Montserret, Roland, Mariño Perez, Laura, Moser, Celine, Muhle-Goll, Claudia, Neves-Martins, Thais Cristtina, Ni, Xiamonin, Norton-Baker, Brenna, Pierattelli, Roberta, Pontoriero, Letizia, Pustovalova, Yulia, Ohlenschläger, Oliver, Orts, Julien, Da Poian, Andrea T., Pyper, Dennis J., Richter, Christian, Riek, Roland, Rienstra, Chad M., Robertson, Angus, Pinheiro, Anderson S., Sabbatella, Raffaele, Salvi, Nicola, Saxena, Krishna, Schulte, Linda, Schiavina, Marco, Schwalbe, Harald, Silber, Mara, Almeida, Marcius Da Silva, Sprague-Piercy, Marc A., Spyroulias, Georgios A., Sreeramulu, Sridhar, Tants, Jan-Niklas, Tārs, Kaspars, Torres, Felix, Töws, Sabrina, Treviño, Miguel Á., Trucks, Sven, Tsika, Aikaterini C., Varga, Krisztina, Wang, Ying, Weber, Marco E., Weigand, Julia E., Wiedemann, Christoph, Wirmer-Bartoschek, Julia, Wirtz Martin, Maria Alexandra, Zehnder, Johannes, Hengesbach, Martin, Schlundt, Andreas

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5529d8e7fcac9fd21390c0b8b064581a

Comprehensive Fragment Screening of the SARS-CoV-2 Proteome Explores Novel Chemical Space for Drug Development.

Autor: Berg H; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Wirtz Martin MA; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Altincekic N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Alshamleh I; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Kaur Bains J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Blechar J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany., Ceylan B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., de Jesus V; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Dhamotharan K; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Strasse 7+9, 60438, Frankfurt am Main, Germany., Fuks C; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany., Gande SL; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Hargittay B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Hohmann KF; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Hutchison MT; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Marianne Korn S; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Strasse 7+9, 60438, Frankfurt am Main, Germany., Krishnathas R; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Kutz F; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Linhard V; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Matzel T; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Meiser N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany., Niesteruk A; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Pyper DJ; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Schulte L; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Trucks S; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany., Azzaoui K; Saverna Therapeutics, Pumpmattenweg 3, 4105, Biel-Benken, Switzerland., Blommers MJJ; Saverna Therapeutics, Pumpmattenweg 3, 4105, Biel-Benken, Switzerland., Gadiya Y; Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Screening Port, Schnackenburgallee 114, 22525, Hamburg, Germany.; Fraunhofer Cluster of Excellence for Immune-Mediated Diseases (CIMD), Theodor-Stern-Kai 7, 60596, Frankfurt am Main, Germany., Karki R; Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Screening Port, Schnackenburgallee 114, 22525, Hamburg, Germany.; Fraunhofer Cluster of Excellence for Immune-Mediated Diseases (CIMD), Theodor-Stern-Kai 7, 60596, Frankfurt am Main, Germany., Zaliani A; Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Screening Port, Schnackenburgallee 114, 22525, Hamburg, Germany.; Fraunhofer Cluster of Excellence for Immune-Mediated Diseases (CIMD), Theodor-Stern-Kai 7, 60596, Frankfurt am Main, Germany., Gribbon P; Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Screening Port, Schnackenburgallee 114, 22525, Hamburg, Germany.; Fraunhofer Cluster of Excellence for Immune-Mediated Diseases (CIMD), Theodor-Stern-Kai 7, 60596, Frankfurt am Main, Germany., da Silva Almeida M; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil.; Fraunhofer Cluster of Excellence for Immune-Mediated Diseases (CIMD), Theodor-Stern-Kai 7, 60596, Frankfurt am Main, Germany., Dinis Anobom C; National Center of Nuclear Magnetic Resonance (CNRMN), CENABIO, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil.; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil., Bula AL; Latvian Institute of Organic Synthesis, Aizkraukles 21, LV-1006, Riga, Latvia., Bütikofer M; ETH, Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, HCI F217, Vladimir-Prelog-Weg 2, 8093, Zürich, Switzerland., Putinhon Caruso Í; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil.; Multiuser Center for Biomolecular Innovation (CMIB), Department of Physics, São Paulo State University (UNESP), 01049-010, São José do Rio Preto, Brazil., Caterina Felli I; Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, 50019, Florence, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3-13, Sesto Fiorentino, 50019, Florence, Italy., Da Poian AT; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil., Cardoso de Amorim G; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil.; Multidisciplinary Center for Research in Biology (NUMPEX), Campus Duque de Caxias Federal University of Rio de Janeiro, 25.250-470, Duque de Caxias, Brazil., Fourkiotis NK; Department of Pharmacy, University of Patras, 26504, Patras, Greece., Gallo A; Department of Pharmacy, University of Patras, 26504, Patras, Greece.; Department of Chemistry, University of Torino, IT-10126, Torino, Italy., Ghosh D; ETH, Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, HCI F217, Vladimir-Prelog-Weg 2, 8093, Zürich, Switzerland., Gomes-Neto F; National Center of Nuclear Magnetic Resonance (CNRMN), CENABIO, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil.; Laboratory of Toxinology, Oswaldo Cruz Foundation (FIOCRUZ), 21040-900, Rio de Janeiro, Brazil., Gorbatyuk O; Department of Molecular Biology and Biophysics, UConn Health, 263 Farmington Ave., Farmington, CT 06030-3305, USA., Hao B; Department of Molecular Biology and Biophysics, UConn Health, 263 Farmington Ave., Farmington, CT 06030-3305, USA., Kurauskas V; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA., Lecoq L; Molecular Microbiology and Structural Biochemistry, UMR5086 CNRS/Université Lyon 1, 7, passage du Vercors, 69367, Lyon, France., Li Y; Department of Molecular Biology and Biophysics, UConn Health, 263 Farmington Ave., Farmington, CT 06030-3305, USA., Cunha Mebus-Antunes N; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil., Mompeán M; 'Rocasolano' Institute for Physical Chemistry, CSIC, 28006, Madrid, Spain., Cristtina Neves-Martins T; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil., Ninot-Pedrosa M; Molecular Microbiology and Structural Biochemistry, UMR5086 CNRS/Université Lyon 1, 7, passage du Vercors, 69367, Lyon, France., Pinheiro AS; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil., Pontoriero L; Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, 50019, Florence, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3-13, Sesto Fiorentino, 50019, Florence, Italy., Pustovalova Y; Department of Molecular Biology and Biophysics, UConn Health, 263 Farmington Ave., Farmington, CT 06030-3305, USA., Riek R; ETH, Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, HCI F217, Vladimir-Prelog-Weg 2, 8093, Zürich, Switzerland., Robertson AJ; Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney, 20892-0520, Bethesda, USA., Jose Abi Saad M; University of Vienna, Department of Pharmaceutical Sciences, Josef-Holaubek-Platz 2, A-1090, Vienna, Austria., Treviño MÁ; 'Rocasolano' Institute for Physical Chemistry, CSIC, 28006, Madrid, Spain., Tsika AC; Department of Pharmacy, University of Patras, 26504, Patras, Greece., Almeida FCL; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil.; National Center of Nuclear Magnetic Resonance (CNRMN), CENABIO, Federal University of Rio de Janeiro, 21941-902, Rio de Janeiro, Brazil., Bax A; Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney, 20892-0520, Bethesda, USA., Henzler-Wildman K; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA., Hoch JC; Department of Molecular Biology and Biophysics, UConn Health, 263 Farmington Ave., Farmington, CT 06030-3305, USA., Jaudzems K; Latvian Institute of Organic Synthesis, Aizkraukles 21, LV-1006, Riga, Latvia., Laurents DV; 'Rocasolano' Institute for Physical Chemistry, CSIC, 28006, Madrid, Spain., Orts J; University of Vienna, Department of Pharmaceutical Sciences, Josef-Holaubek-Platz 2, A-1090, Vienna, Austria., Pierattelli R; Magnetic Resonance Center (CERM), University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, 50019, Florence, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3-13, Sesto Fiorentino, 50019, Florence, Italy., Spyroulias GA; Department of Pharmacy, University of Patras, 26504, Patras, Greece., Duchardt-Ferner E; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Strasse 7+9, 60438, Frankfurt am Main, Germany., Ferner J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Fürtig B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Hengesbach M; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany., Löhr F; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Strasse 7+9, 60438, Frankfurt am Main, Germany., Qureshi N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Richter C; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Saxena K; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Schlundt A; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Strasse 7+9, 60438, Frankfurt am Main, Germany., Sreeramulu S; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Wacker A; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Weigand JE; Department of Biology, Technical University of Darmstadt, 64289, Darmstadt, Germany., Wirmer-Bartoschek J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany., Wöhnert J; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Strasse 7+9, 60438, Frankfurt am Main, Germany., Schwalbe H; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main (Germany), Max-von-LaueStrasse 7+9, 60438, Frankfurt am Main, Germany.

Publikováno v: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2022 Nov 14; Vol. 61 (46), pp. e202205858. Date of Electronic Publication: 2022 Oct 13.

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications.

Autor: Altincekic N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Korn SM; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Qureshi NS; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Dujardin M; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Ninot-Pedrosa M; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Abele R; Institute for Biochemistry, Goethe University Frankfurt, Frankfurt am Main, Germany., Abi Saad MJ; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Alfano C; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Almeida FCL; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Alshamleh I; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., de Amorim GC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Multidisciplinary Center for Research in Biology (NUMPEX), Campus Duque de Caxias Federal University of Rio de Janeiro, Duque de Caxias, Brazil., Anderson TK; Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI, United States., Anobom CD; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Anorma C; Department of Chemistry, University of California, Irvine, CA, United States., Bains JK; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Bax A; LCP, NIDDK, NIH, Bethesda, MD, United States., Blackledge M; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Blechar J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Böckmann A; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Brigandat L; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Bula A; Latvian Institute of Organic Synthesis, Riga, Latvia., Bütikofer M; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Camacho-Zarco AR; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Carlomagno T; BMWZ and Institute of Organic Chemistry, Leibniz University Hannover, Hannover, Germany.; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Braunschweig, Germany., Caruso IP; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Multiuser Center for Biomolecular Innovation (CMIB), Department of Physics, São Paulo State University (UNESP), São José do Rio Preto, Brazil., Ceylan B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Chaikuad A; Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.; Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany., Chu F; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Cole L; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Crosby MG; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., de Jesus V; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Dhamotharan K; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Felli IC; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Ferner J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Fleischmann Y; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Fogeron ML; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Fourkiotis NK; Department of Pharmacy, University of Patras, Patras, Greece., Fuks C; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Fürtig B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Gallo A; Department of Pharmacy, University of Patras, Patras, Greece., Gande SL; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Gerez JA; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Ghosh D; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Gomes-Neto F; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Laboratory of Toxinology, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil., Gorbatyuk O; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Guseva S; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Hacker C; Signals GmbH & Co. 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Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Bessa LM; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Martin RW; Department of Chemistry, University of California, Irvine, CA, United States.; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., Matzel T; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Maurin D; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., McNutt SW; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Mebus-Antunes NC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Meier BH; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Meiser N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Mompeán M; 'Rocasolano' Institute for Physical Chemistry (IQFR), Spanish National Research Council (CSIC), Madrid, Spain., Monaca E; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Montserret R; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Mariño Perez L; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Moser C; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Muhle-Goll C; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Neves-Martins TC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Ni X; Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.; Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany., Norton-Baker B; Department of Chemistry, University of California, Irvine, CA, United States., Pierattelli R; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Pontoriero L; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Pustovalova Y; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Ohlenschläger O; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Orts J; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Da Poian AT; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Pyper DJ; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Richter C; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Riek R; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Rienstra CM; Department of Biochemistry and National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI, United States., Robertson A; LCP, NIDDK, NIH, Bethesda, MD, United States., Pinheiro AS; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Sabbatella R; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Salvi N; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Saxena K; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Schulte L; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Schiavina M; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Schwalbe H; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Silber M; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Almeida MDS; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Sprague-Piercy MA; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., Spyroulias GA; Department of Pharmacy, University of Patras, Patras, Greece., Sreeramulu S; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Tants JN; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Tārs K; Latvian Biomedical Research and Study Centre, Riga, Latvia., Torres F; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Töws S; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Treviño MÁ; 'Rocasolano' Institute for Physical Chemistry (IQFR), Spanish National Research Council (CSIC), Madrid, Spain., Trucks S; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Tsika AC; Department of Pharmacy, University of Patras, Patras, Greece., Varga K; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Wang Y; BMWZ and Institute of Organic Chemistry, Leibniz University Hannover, Hannover, Germany., Weber ME; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Weigand JE; Department of Biology, Technical University of Darmstadt, Darmstadt, Germany., Wiedemann C; Institute of Biochemistry and Biotechnology, Charles Tanford Protein Centre, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany., Wirmer-Bartoschek J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Wirtz Martin MA; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Zehnder J; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Hengesbach M; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Schlundt A; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany.

Publikováno v: Frontiers in molecular biosciences [Front Mol Biosci] 2021 May 10; Vol. 8, pp. 653148. Date of Electronic Publication: 2021 May 10 (Print Publication: 2021).