Zobrazeno 1 - 10
of 182
pro vyhledávání: '"Fluorophore-assisted carbohydrate electrophoresis"'
Autor:
Masahiro Kimura, Takatoshi Umeyama, Satoshi Wakita, Kazuaki Okawa, Masayoshi Sakaguchi, Vaclav Matoska, Peter O. Bauer, Fumitaka Oyama
Publikováno v:
MethodsX, Vol 7, Iss , Pp 100881- (2020)
Fluorophore-assisted carbohydrate electrophoresis (FACE) enables detection and quantification of degradation products from artificial and natural chitin substrates such as 4-NP-(GlcNAc)2, (GlcNAc)4 and colloidal chitin. The FACE method has been impro
Externí odkaz:
https://doaj.org/article/b87959e273754f49b78e62d76986b03b
Autor:
Evgenia Karousou, Athanasia Asimakopoulou, Luca Monti, Vassiliki Zafeiropoulou, Nikos Afratis, Panagiotis Gartaganis, Antonio Rossi, Alberto Passi, Nikos K. Karamanos
Publikováno v:
Molecules, Vol 19, Iss 6, Pp 7959-7980 (2014)
Glycosaminoglycans (GAGs) due to their hydrophilic character and high anionic charge densities play important roles in various (patho)physiological processes. The identification and quantification of GAGs in biological samples and tissues could be us
Externí odkaz:
https://doaj.org/article/174057803b11437cbf7bc2bd1acb87c4
Autor:
Theresa Dutschei, Uwe T. Bornscheuer, Christoph Suster, Nadine Gerlach, Thomas Schweder, Daniel Bartosik, Jan-Hendrik Hehemann, Marcus Bäumgen, Marko D. Mihovilovic, Christian Stanetty, Lukas Reisky
Publikováno v:
The Journal of Biological Chemistry
JOURNAL OF BIOLOGICAL CHEMISTRY
JOURNAL OF BIOLOGICAL CHEMISTRY
Marine algae catalyze half of all global photosynthetic production of carbohydrates. Owing to their fast growth rates, Ulva spp. rapidly produce substantial amounts of carbohydrate-rich biomass and represent an emerging renewable energy and carbon re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66ec3eb176348d6960b075ccc8032d2a
https://pubmed.ncbi.nlm.nih.gov/34547290
https://pubmed.ncbi.nlm.nih.gov/34547290
Akademický článek
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Akademický článek
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Publikováno v:
European Cells & Materials, Vol 19, Pp 86-95 (2010)
The injectable and hydrophilic nature of hydrogels makes them suitable candidates for cartilage tissue engineering. To date, a wide range of hydrogels have been proposed for articular cartilage regeneration but few studies have quantitatively compare
Externí odkaz:
https://doaj.org/article/ce22bf5ed70b4be0904c982e5deff7dc
Autor:
Liping Tian, Suzy A. A. Comhair, James Hiznay, Jarrod W. Barnes, Jacqueline Loftis, Raed A. Dweik, Mark E. Lauer
Publikováno v:
MethodsX
Graphical abstract
The building blocks of simple and complex oligosaccharides, termed sugar nucleotides, are often overlooked for their role in metabolic diseases and may hold the key to the underlying disease pathogenesis. Multiple reasons may
The building blocks of simple and complex oligosaccharides, termed sugar nucleotides, are often overlooked for their role in metabolic diseases and may hold the key to the underlying disease pathogenesis. Multiple reasons may
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::879ff078430224727d3ba8f2b4858b03
Autor:
Fumitaka Oyama, Kazuaki Okawa, Takatoshi Umeyama, Vaclav Matoska, Masayoshi Sakaguchi, Satoshi Wakita, Masahiro Kimura, Peter Bauer
Publikováno v:
MethodsX
MethodsX, Vol 7, Iss, Pp 100881-(2020)
MethodsX, Vol 7, Iss, Pp 100881-(2020)
Highlights • FACE is a simple, qualitative and quantitative method. • The standard curve warrants quantification of chitooligosaccharides of up to 10 nmol regardless on used buffer system. • Our improved FACE method enable us to quantify chitoo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfa709e4f45115f5019161b499bcc076
https://pubmed.ncbi.nlm.nih.gov/32346528
https://pubmed.ncbi.nlm.nih.gov/32346528
Publikováno v:
Methods in Molecular Biology ISBN: 9781493992126
DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis allows for accurate profiling of the asparagine-linked (N-) glycosylation patterns, a posttranslational modification present on many soluble and membrane proteins. This techniqu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::48201351971ff760d55b7a71ae7039fb
https://doi.org/10.1007/978-1-4939-9213-3_17
https://doi.org/10.1007/978-1-4939-9213-3_17
Hyaluronan (HA) exhibits numerous important roles in physiology and pathologies, and these facts necessitate an ability to accurately and reproducibly measure its quantities in tissues and cell cultures. Our group previously reported a rigorous and a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f0355c33e4d4e4d1e7e9ec0e668fcaec
https://doi.org/10.1016/bs.mcb.2017.08.017
https://doi.org/10.1016/bs.mcb.2017.08.017