Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Fluorescent in situ sequencing"'
Autor:
Richie E. Kohman, George M. Church
Biological tissues contain thousands of different proteins yet conventional antibody staining can only assay a few at a time because of the limited number of spectrally distinct fluorescent labels. The capacity to map the location of hundreds or thou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0cb413e280902e0d29bae1151a50e90
https://doi.org/10.1101/2020.04.27.060624
https://doi.org/10.1101/2020.04.27.060624
Autor:
Octavian Bucur
Publikováno v:
Discoveries
Many technological advances have been made in the recent years, several of them with a great potential of significantly improving the diagnostic pathology field. This article discusses three of the most promising technologies, which emerged in the la
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c605dc01029caac9c1abaed41a71434e
https://pubmed.ncbi.nlm.nih.gov/32309569
https://pubmed.ncbi.nlm.nih.gov/32309569
Publikováno v:
Nature methods
We present here an approach for engineering evolving DNA barcodes in living cells. The methodology entails using a homing guide RNA (hgRNA) scaffold that directs the Cas9-hgRNA complex to target the DNA locus of the hgRNA itself. We show that this ho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3dfea99303342abde52a721f0a7dd3a
https://doi.org/10.1038/nmeth.4108
https://doi.org/10.1038/nmeth.4108
Autor:
Je H. Lee
Publikováno v:
Wiley Interdisciplinary Reviews. Systems Biology and Medicine
The spatial information associated with gene expression is important for elucidating the context‐dependent transcriptional regulation during development. Recently, high‐resolution sampling approaches, such as RNA tomography or single‐cell RNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0261e9269c3e8cb6447d70eeaa9d3020
https://doi.org/10.1002/wsbm.1369
https://doi.org/10.1002/wsbm.1369
Publikováno v:
Recent Patents on Biomedical Engineeringe. 1:60-67
We are now witnessing a new genomic revolution due to the arrival and continued advancements in the next-generation high-throughput sequencing technologies, which encompass sequencing by synthesis including fluorescent in situ sequencing (FISSEQ) and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c6adf75b7c6d74f89ba3bc9bb4e52f7
https://doi.org/10.2174/1874764710801010060
https://doi.org/10.2174/1874764710801010060
Autor:
Thomas C. Ferrante, George M. Church, Jonathan Scheiman, Joyce L. Yang, Brian M. Turczyk, Reza Kalhor, Kun Zhang, Richard C. Terry, Evan R. Daugharthy, Hosuk Lee, John Aach, Je-Hyuk Lee
RNA-sequencing (RNA-seq) measures the quantitative change in gene expression over the whole transcriptome, but it lacks spatial context. In contrast, in situ hybridization provides the location of gene expression, but only for a small number of genes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26f8774dac3f27e2285eaaf46c30bb40
https://europepmc.org/articles/PMC4327781/
https://europepmc.org/articles/PMC4327781/
Publikováno v:
Analytical Biochemistry. 320:55-65
Integration of DNA isolation, amplification, and sequencing can be achieved by the use of polymerase colonies (polonies) and cycles of fluorescent dNTP incorporation. In this paper, we present four advances that bring us closer to sequencing genomes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6d4167e99100d434a195efc8bca9eaf
https://doi.org/10.1016/s0003-2697(03)00291-4
https://doi.org/10.1016/s0003-2697(03)00291-4
Erratum to 'Fluorescent in situ sequencing on polymerase colonies' [Anal. Biochem. 320 (2003) 55–65]
Publikováno v:
Analytical Biochemistry. 328:245
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f8b90d2c1f00599c760925465459b7f8
https://doi.org/10.1016/j.ab.2004.03.002
https://doi.org/10.1016/j.ab.2004.03.002