Zobrazeno 1 - 10
of 541
pro vyhledávání: '"Fluorescence Resonance Energy Transfer/methods"'
Publikováno v:
Seibutsu Butsuri. 42:S33
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fa5cc1d077560ead4c1059c1515a1a94
https://doi.org/10.2142/biophys.42.s33_2
https://doi.org/10.2142/biophys.42.s33_2
Autor:
Néstor Sampedro Vallina, Ewan K S McRae, Bente Kring Hansen, Adrien Boussebayle, Ebbe Sloth Andersen
Publikováno v:
Sampedro Vallina, N, McRae, E K S, Hansen, B K, Boussebayle, A & Andersen, E S 2023, ' RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli-Pepper aptamer FRET pair ', Nucleic Acids Research, vol. 51, no. 9, pp. 4613-4624 . https://doi.org/10.1093/nar/gkad224
Sampedro Vallina, N, McRae, E K S, Hansen, B K, Boussebayle, A & Andersen, E S 2023, ' RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli-Pepper aptamer FRET pair ', Nucleic Acids Research . https://doi.org/10.1093/nar/gkad224
Sampedro Vallina, N, McRae, E K S, Hansen, B K, Boussebayle, A & Andersen, E S 2023, ' RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli-Pepper aptamer FRET pair ', Nucleic Acids Research . https://doi.org/10.1093/nar/gkad224
Cryogenic electron microscopy (cryo-EM) is a promising method for characterizing the structure of larger RNA structures and complexes. However, the structure of individual aptamers is difficult to solve by cryo-EM due to their low molecular weight an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c2a22c125f5e7bd61d4b18b2a50edc1
https://pure.au.dk/portal/da/publications/rna-origami-scaffolds-facilitate-cryoem-characterization-of-a-broccolipepper-aptamer-fret-pair(d8f53ecb-3a05-4f1f-ad94-3c644bceb46c).html
https://pure.au.dk/portal/da/publications/rna-origami-scaffolds-facilitate-cryoem-characterization-of-a-broccolipepper-aptamer-fret-pair(d8f53ecb-3a05-4f1f-ad94-3c644bceb46c).html
Autor:
Wu, Sheng-Yi, Wen, Yurong, Serre, Nelson B. C., Laursen, Cathrine Charlotte Heiede, Dietz, Andrea Grostøl, Taylor, Brian R., Drobizhev, Mikhail, Molina, Rosana S., Aggarwal, Abhi, Rancic, Vladimir, Becker, Michael, Ballanyi, Klaus, Podgorski, Kaspar, Hirase, Hajime, Nedergaard, Maiken, Fendrych, Matyáš, Lemieux, M. Joanne, Eberl, Daniel F., Kay, Alan R., Campbell, Robert E., Shen, Yi
Publikováno v:
Wu, S-Y, Wen, Y, Serre, N B C, Laursen, C C H, Dietz, A G, Taylor, B R, Drobizhev, M, Molina, R S, Aggarwal, A, Rancic, V, Becker, M, Ballanyi, K, Podgorski, K, Hirase, H, Nedergaard, M, Fendrych, M, Lemieux, M J, Eberl, D F, Kay, A R, Campbell, R E & Shen, Y 2023, ' A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life ', PLOS Biology, vol. 20, no. 9, e3001772 . https://doi.org/10.1371/journal.pbio.3001772
Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and patholog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fad25e3f62055468038197c8f35baa9
https://curis.ku.dk/portal/da/publications/a-sensitive-and-specific-geneticallyencoded-potassium-ion-biosensor-for-in-vivo-applications-across-the-tree-of-life(a8b75c50-b089-4f09-affb-793d45254f4f).html
https://curis.ku.dk/portal/da/publications/a-sensitive-and-specific-geneticallyencoded-potassium-ion-biosensor-for-in-vivo-applications-across-the-tree-of-life(a8b75c50-b089-4f09-affb-793d45254f4f).html
Publikováno v:
Nielsen, L D F, Hansen-Bruhn, M, Nijenhuis, M A D & Gothelf, K V 2022, ' Protein-Induced Fluorescence Enhancement and Quenching in a Homogeneous DNA-Based Assay for Rapid Detection of Small-Molecule Drugs in Human Plasma ', ACS Sensors, vol. 7, no. 3, pp. 856-865 . https://doi.org/10.1021/acssensors.1c02642
Homogeneous assays for determining the concentration of small molecules in biological fluids are of importance for monitoring blood levels of critical drugs in patients. We have developed a strand displacement competition assay for the drugs dabigatr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64b94d237417fede92527b70b30cc05c
https://pure.au.dk/ws/files/296489745/IM_Protein_Induced_Fluorescence_Enhancement_and_Quenching_in_a_Homogeneous_DNA_Based_Assay_for_Rapid_Detection_of_Small_Molecule_Drugs_in_Human_Plasma.pdf
https://pure.au.dk/ws/files/296489745/IM_Protein_Induced_Fluorescence_Enhancement_and_Quenching_in_a_Homogeneous_DNA_Based_Assay_for_Rapid_Detection_of_Small_Molecule_Drugs_in_Human_Plasma.pdf
Publikováno v:
Methods in Enzymology. 589:87-114
FRET-sensors have become important tools for intracellular imaging, but their dependence on external illumination presents some limitations, such as photobleaching and phototoxicity, which limit measurements over extended periods of time. Fluorescenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=dris___01180::8469606efb8d09a7929c37b4d28feef1
https://research.tue.nl/en/publications/e50352a8-49a6-41db-a373-fb3d82ba142a
https://research.tue.nl/en/publications/e50352a8-49a6-41db-a373-fb3d82ba142a
Publikováno v:
Methods in Enzymology, 589, 87-114. Academic Press Inc.
FRET-sensors have become important tools for intracellular imaging, but their dependence on external illumination presents some limitations, such as photobleaching and phototoxicity, which limit measurements over extended periods of time. Fluorescenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=narcis______::dbccab4ed35daae1b051b88b430426ae
https://research.tue.nl/nl/publications/e50352a8-49a6-41db-a373-fb3d82ba142a
https://research.tue.nl/nl/publications/e50352a8-49a6-41db-a373-fb3d82ba142a
Publikováno v:
The Journal of Biological Chemistry
The Journal of biological chemistry
Journal of Biological Chemistry, Vol. 286, No 42 (2011) pp. 36448-59
The Journal of biological chemistry
Journal of Biological Chemistry, Vol. 286, No 42 (2011) pp. 36448-59
Background: STIM1 oligomerization upon endoplasmic reticulum (ER) Ca2+ depletion activates store-operated Ca2+ entry (SOCE) channels, but whether this mechanism is reversible is unknown. Results: STIM1 de-oligomerization upon ER Ca2+ refilling requir
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1239165f14308202827d94c5c9e9c34d
https://doi.org/10.1074/jbc.m111.269415
https://doi.org/10.1074/jbc.m111.269415
Autor:
Favaloro, Emmanuel J.1,2,3 (AUTHOR) emmanuel.favaloro@health.nsw.gov.au, Pasalic, Leonardo1,4 (AUTHOR)
Publikováno v:
Diagnostics (2075-4418). Nov2024, Vol. 14 Issue 22, p2521. 19p.
Autor:
Cécile Thomas, Jean-François Margathe, Elodie Dupuis, Nadia Oueslati, Thierry Durroux, Eric Trinquet, Jean-Philippe Pin, Didier Rognan, Marcel Hibert, Candide Hounsou, Dominique Bonnet, André Mann, Brigitte Ilien, Abderazak Belhocine
Publikováno v:
ACS Chemical Biology
ACS Chemical Biology, American Chemical Society, 2015, 10 (2), pp.466--74. ⟨10.1021/cb5007568⟩
ACS Chemical Biology, American Chemical Society, 2015, 10 (2), pp.466--74. ⟨10.1021/cb5007568⟩
International audience; G protein-coupled receptors (GPCRs) have been described to form hetero-oligomers. The importance of these complexes in physiology and pathology is considered crucial, and heterodimers represent promising new targets to discove
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dc91abd537109e05b23b74463fb054a
https://pubmed.ncbi.nlm.nih.gov/25350273
https://pubmed.ncbi.nlm.nih.gov/25350273
Autor:
Gao, Chunyan1 (AUTHOR), Gao, Qinghua1 (AUTHOR), Zhao, Cong1 (AUTHOR), Huo, Yani1 (AUTHOR), Zhang, Zhizhuo1 (AUTHOR), Yang, Jinlong2 (AUTHOR) jlyang@ustc.edu.cn, Jia, Chuancheng1 (AUTHOR) jiacc@nankai.edu.cn, Guo, Xuefeng1,3 (AUTHOR) guoxf@pku.edu.cn
Publikováno v:
National Science Review. Aug2024, Vol. 11 Issue 8, p1-31. 31p.