Zobrazeno 1 - 10
of 1 069
pro vyhledávání: '"Flow cytometry/Mass cytometry"'
Autor:
Kohei Yamada, Jaivardhan A. Menon, Yaunghyun Kim, Changde Cheng, Wenan Chen, Jenny A. Shih, Ana B. Villasenor-Altamirano, Xiang Chen, Tomoyoshi Tamura, Louis T. Merriam, Edy Y. Kim, Alexandra J. Weissman
Publikováno v:
STAR Protocols, Vol 5, Iss 1, Pp 102874- (2024)
Summary: Immunophenotyping of out-of-hospital cardiac arrest (OHCA) patients is of increasing interest but has challenges. Here, we describe steps for the design of the clinical cohort, planning patient enrollment and sample collection, and ethical r
Externí odkaz:
https://doaj.org/article/a33b1ff513a643bf841618340b656c10
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102620- (2023)
Summary: Mucosal-associated invariant T (MAIT) cells are innate-like T cells responsible for mucosal immunity in the respiratory and intestinal tracts. Here we present a flow-cytometry-based assay to measure the cytolytic activity of murine MAIT cell
Externí odkaz:
https://doaj.org/article/cbd51081d3174826be42f05622b67723
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102638- (2023)
Summary: Efficient skeletal muscle regeneration necessitates fine-tuned coordination among multiple cell types through an intricate network of intercellular communication. We present a protocol for generation of a time-resolved cellular interactome d
Externí odkaz:
https://doaj.org/article/da595b1e7d6242c5a5e5a589d590d3e0
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102582- (2023)
Summary: T cells are able to recognize and kill pathogens that infect host cells, including bacteria, viruses, and tumor cells. Here, we present a protocol to detect T cell function and bacterial load in OVA-Listeria monocytogenes-infected mice. We p
Externí odkaz:
https://doaj.org/article/381441f761634fc8b99f1910f67eaae5
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102608- (2023)
Summary: Parasitic helminth worms frequently infect the gastrointestinal tract and interact with the intestinal epithelium and specialized cell types within it. Intestinal organoids derived from stem cells that line the intestine represent a transfor
Externí odkaz:
https://doaj.org/article/3af02bea6fe145e2ae887bd29fdf4435
Autor:
Ehsan Bahrami, Tobias Geiger, Agnes A. Steixner-Kumar, Diana Santacruz, Coralie Viollet, Alec Dick, Yvonne Roth, Patrycja Schlingeloff, Julian Schmidberger, Mark Haenle, Wolfgang Kratzer, Kerstin Kitt, Heike Neubauer, Eric Simon, Oliver Krenkel, Markus Werner
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102597- (2023)
Summary: Immune dysregulation and inflammation by hepatic-resident leukocytes is considered a key step in disease progression of non-alcoholic fatty liver disease and non-alcoholic steatohepatitis toward cirrhosis and hepatocellular carcinoma. Here,
Externí odkaz:
https://doaj.org/article/5048d44204c442c6b3b41d90644ec224
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102592- (2023)
Summary: There are numerous established techniques for isolating hepatic myeloid cells; however, preserving their phenotypic and functional characteristics can be challenging. We present a straightforward and efficient method to isolate hepatic myelo
Externí odkaz:
https://doaj.org/article/abde43627e0f407e826b55ee7fc4a4dd
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102599- (2023)
Summary: Astrocytes are glial cells of the central nervous system that modulate neuronal function. Here, we present glyoxal-fixed astrocyte nuclei transcriptomics (GFAT), a protocol for the purification and transcriptomic analysis of astrocyte nuclei
Externí odkaz:
https://doaj.org/article/59514848365044b6a3e4350edb150492
Autor:
Changzheng Li, Jingjing Guan, Yishan Li, Xiaobin Tian, Yijun Zhao, Weiming Liu, Huixuan Tian, Huiqi Tian, Yalan Yang, Meng Zhao
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102578- (2023)
Summary: Hematopoietic stem cells (HSCs) sustain hematopoiesis during homeostasis and regeneration. However, their limited availability poses a challenge for protein analysis. Here, we present a protocol for performing high-sensitivity western blot o
Externí odkaz:
https://doaj.org/article/db55c173a759408f82909c07d7c847e3
Autor:
Camilla Tiezzi, Marzia Rossi, Andrea Vecchi, Sara Doselli, Amalia Penna, Paola Fisicaro, Ilaria Montali, Camilla Ceccatelli Berti, Valentina Reverberi, Anna Montali, Gabriele Missale, Carlo Ferrari, Carolina Boni
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102584- (2023)
Summary: Monitoring antigen-specific T cell frequency and function is essential to assess the host immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we present a FluoroSpot assay for concurrently detecti
Externí odkaz:
https://doaj.org/article/1f4b8be0526e456ab475800c1653cc70