Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Florian W, Krainer"'
Publikováno v:
Hosford, J, Valles, M, Krainer, F W, Glieder, A & Wong, L S 2018, ' Parallelized Biocatalytic Scanning Probe Lithography for the Additive Fabrication of Conjugated Polymer Structures ', Nanoscale, vol. 10, pp. 7185-7193 . https://doi.org/10.1039/C8NR01283K
Scanning probe lithography (SPL) offers a more accessible alternative to conventional photolithography as a route to surface nanofabrication. In principle, the synthetic scope of SPL could be greatly enhanced by combining the precision of scanning pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::074c94ebd68071551a79e32e1e1f6f9c
https://www.research.manchester.ac.uk/portal/en/publications/parallelized-biocatalytic-scanning-probe-lithography-for-the-additive-fabrication-of-conjugated-polymer-structures(954558a0-78a4-4c59-aea6-2b0c4d7fc97c).html
https://www.research.manchester.ac.uk/portal/en/publications/parallelized-biocatalytic-scanning-probe-lithography-for-the-additive-fabrication-of-conjugated-polymer-structures(954558a0-78a4-4c59-aea6-2b0c4d7fc97c).html
Autor:
Florian W, Krainer, Michaela A, Gerstmann, Barbara, Darnhofer, Ruth, Birner-Gruenberger, Anton, Glieder
Publikováno v:
Journal of biotechnology. 233
Horseradish peroxidase (HRP) is a high-demand enzyme for applications in diagnostics, bioremediation, biocatalysis and medicine. Current HRP preparations are isolated from horseradish roots as mixtures of biochemically diverse isoenzymes. Thus, there
Autor:
Benjamin S. Glick, Martina Geier, Lukas Sturmberger, Knut Madden, Florian W. Krainer, Ruth Birner-Gruenberger, Ilya I. Tolstorukov, Ursa Vide, Anja Schiefer, Toby Richardson, Barbara Darnhofer, Kasey J. Day, Leah Soriaga, Anton Glieder, James M. Cregg, Sara Trstenjak, Chappell Thomas G
Strains of the species Komagataella phaffii are the most frequently used “Pichia pastoris” strains employed for recombinant protein production as well as studies on peroxisome biogenesis, autophagy and secretory pathway analyses. Genome sequencin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::349581f2cbbb63570321475113143778
https://europepmc.org/articles/PMC5089815/
https://europepmc.org/articles/PMC5089815/
Publikováno v:
Microbial Cell Factories
Background Tagging proteins is a standard method facilitating protein detection, purification or targeting. When tagging a certain protein of interest, it is challenging to predict which tag will give optimal results and will not interfere with prote
Autor:
Florian W, Krainer, Simona, Capone, Martin, Jäger, Thomas, Vogl, Michaela, Gerstmann, Anton, Glieder, Christoph, Herwig, Oliver, Spadiut
Publikováno v:
Microbial Cell Factories
Background Insufficient incorporation of heme is considered a central impeding cause in the recombinant production of active heme proteins. Currently, two approaches are commonly taken to overcome this bottleneck; metabolic engineering of the heme bi
An updated view on horseradish peroxidases: recombinant production and biotechnological applications
Autor:
Florian W, Krainer, Anton, Glieder
Publikováno v:
Applied Microbiology and Biotechnology
Horseradish peroxidase has been the subject of scientific research for centuries. It has been used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a major role in these applications. Numerous studies have been conduc
Publikováno v:
BMC Genomics
Background Horseradish peroxidases (HRPs) from Armoracia rusticana have long been utilized as reporters in various diagnostic assays and histochemical stainings. Regardless of their increasing importance in the field of life sciences and suggested us
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cae37f10e06d90cb182268f93235c1d
http://openlib.tugraz.at/564f252f212d0
http://openlib.tugraz.at/564f252f212d0
Autor:
Florian W, Krainer, Robert, Pletzenauer, Laura, Rossetti, Christoph, Herwig, Anton, Glieder, Oliver, Spadiut
Publikováno v:
Protein Expression and Purification
Highlights • A novel 2-step purification for recombinant HRP from P. pastrois was developed. • Both purification steps are negative chromatography steps. • The amount of N-glycosylation sites and the success in purification correlate. • HRP i
Autor:
Florian W, Krainer, Christoph, Gmeiner, Lukas, Neutsch, Markus, Windwarder, Robert, Pletzenauer, Christoph, Herwig, Friedrich, Altmann, Anton, Glieder, Oliver, Spadiut
Publikováno v:
Scientific Reports
The yeast Pichia pastoris is a common host for the recombinant production of biopharmaceuticals, capable of performing posttranslational modifications like glycosylation of secreted proteins. However, the activity of the OCH1 encoded α-1,6-mannosylt
Autor:
Christian Dietzsch, Tanja Hajek, Anton Glieder, Christoph Herwig, Oliver Spadiut, Florian W. Krainer
Publikováno v:
Microbial Cell Factories
Microbial Cell Factories, Vol 11, Iss 1, p 22 (2012)
Microbial Cell Factories, Vol 11, Iss 1, p 22 (2012)
Βackground The methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production and is able to use methanol as a sole carbon source. The methanol utilization pathway describes all the catalytic reactions,