Výsledky vyhledávání - "Florian Semmelmann"

Akademický článek

Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli

Autor: Bettina Rohweder, Florian Semmelmann, Christiane Endres, Reinhard Sterner

Publikováno v: BioTechniques, Vol 64, Iss 1, Pp 24-26 (2018)

Here, we modified the multiple cloning sites from commonly used expression vectors to create a new suite of cloning plasmids that simplify and speed up cloning procedures in Escherichia coli. Each of our standardized plasmids contains two BsaI restri

Externí odkaz: https://doaj.org/article/842c82dc61e948648a5c8f9c314fabe6

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Mapping the Allosteric Communication Network of Aminodeoxychorismate Synthase

Autor: Florian Semmelmann, Chitra Rajendran, Reinhard Sterner, Kristina Straub, Rainer Merkl, Julian Nazet

Publikováno v: Journal of Molecular Biology. 431:2718-2728

Allosteric communication between different subunits in metabolic enzyme complexes is of utmost physiological importance but only understood for few systems. We analyzed the structural basis of allostery in aminodeoxychorismate synthase (ADCS), which

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84e7063fff6f6e5303f53ea12f233a62
https://doi.org/10.1016/j.jmb.2019.05.021

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Reprogramming the Specificity of a Protein Interface by Computational and Data-Driven Design

Autor: Rainer Merkl, Florian Semmelmann, Franziska Funke, Reinhard Sterner, Regina Hertle, Julian Nazet, Sandra Schlee

Publikováno v: Structure (London, England : 1993). 29(3)

Summary The formation of specific protein complexes in a cell is a non-trivial problem given the co-existence of thousands of different polypeptide chains. A particularly difficult case are two glutamine amidotransferase complexes (anthranilate synth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f21c2541dfda365599f42d513b7df35f
https://pubmed.ncbi.nlm.nih.gov/33296666

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Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli

Autor: Reinhard Sterner, Florian Semmelmann, Bettina Rohweder, Christiane Endres

Publikováno v: BioTechniques. 64:24-26

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c5072f2d2567d5516ef28ce1f700af7
https://doi.org/10.2144/000114628

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A Fold-Independent Interface Residue Is Crucial for Complex Formation and Allosteric Signaling in Class I Glutamine Amidotransferases

Autor: Rainer Merkl, Reinhard Sterner, Enrico Hupfeld, Florian Semmelmann, Leonhard Heizinger

Publikováno v: Biochemistry. 58(22)

The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme compl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76aacc85fc6dd89eb6d88d1c5c8b302a
https://pubmed.ncbi.nlm.nih.gov/31117390

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Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor inNasonia

Autor: Jacintha Ellers, Florian Semmelmann, Bastian Broschwitz, Naoki Kabeya, Joachim Ruther, Kristina Straub, Astrid Bruckmann, Miriama Malcicka, Rainer Merkl, Reinhard Sterner, Óscar Monroig

Publikováno v: The Journal of experimental biology, 222(10):jeb201038, 1-8. Company of Biologists Ltd
Digital.CSIC. Repositorio Institucional del CSIC
instname
Semmelmann, F, Kabeya, N, Malcicka, M, Bruckmann, A, Broschwitz, B, Straub, K, Merkl, R, Monroig, O, Sterner, R, Ruther, J & Ellers, J 2019, ' Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia ', The Journal of experimental biology, vol. 222, no. 10, jeb201038, pp. 1-8 . https://doi.org/10.1242/jeb.201038

Insect pheromones are often derived from fatty acid metabolism. Fatty acid desaturases, enzymes introducing double bonds into fatty acids, are crucial for the biosynthesis of these chemical signals. Δ12-desaturases catalyse the biosynthesis of linol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6bf7fa81485f8777f609214d463aa9e
https://doi.org/10.1242/jeb.201038

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Evolutionary diversification of protein–protein interactions by interface add-ons

Autor: Markus Busch, Leonhard Heizinger, Florian Busch, Rainer Merkl, Maximilian G. Plach, Florian Semmelmann, Vicki H. Wysocki, Reinhard Sterner

Publikováno v: Proceedings of the National Academy of Sciences. 114

Cells contain a multitude of protein complexes whose subunits interact with high specificity. However, the number of different protein folds and interface geometries found in nature is limited. This raises the question of how protein-protein interact

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fa5be9138207b1e57152fc63822dba3
https://doi.org/10.1073/pnas.1707335114

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Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia

Autor: Joachim, Ruther, Åsa K, Hagström, Birgit, Brandstetter, John, Hofferberth, Astrid, Bruckmann, Florian, Semmelmann, Michaela, Fink, Helena, Lowack, Sabine, Laberer, Oliver, Niehuis, Rainer, Deutzmann, Christer, Löfstedt, Reinhard, Sterner

Publikováno v: Scientific reports, 6:34697
Scientific Reports

Males of all species of the parasitic wasp genus Nasonia use (4R,5S)-5-hydroxy-4-decanolide (RS) as component of their sex pheromone while only N. vitripennis (Nv), employs additionally (4R,5R)-5-hydroxy-4-decanolide (RR). Three genes coding for the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0da3817a095cb785f91786f7f5d50d6a
https://www.nature.com/articles/srep34697#supplementary-information

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Development of a highly thermostable, adjuvanted human papillomavirus vaccine

Autor: Florian Semmelmann, Natalie M. Meinerz, Theodore W. Randolph, Megan C. Cousins, Kimberly J. Hassett, Robert L. Garcea

Publikováno v: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 94

A major impediment to economical, worldwide vaccine distribution is the requirement for a “cold chain” to preserve antigenicity. We addressed this problem using a model human papillomavirus (HPV) vaccine stabilized by immobilizing HPV16 L1 capsom

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a14ea0cea592c9a340f02be7843d82d6
https://pubmed.ncbi.nlm.nih.gov/25998700

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