Multi-omics characterization of the monkeypox virus infection

Autor: Yiqi Huang, Valter Bergant, Vincent Grass, Quirin Emslander, M. Sabri Hamad, Philipp Hubel, Julia Mergner, Antonio Piras, Karsten Krey, Alexander Henrici, Rupert Öllinger, Yonas M. Tesfamariam, Ilaria Dalla Rosa, Till Bunse, Gerd Sutter, Gregor Ebert, Florian I. Schmidt, Michael Way, Roland Rad, Andrew G. Bowie, Ulrike Protzer, Andreas Pichlmair

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-19 (2024)

Abstract Multiple omics analyzes of Vaccinia virus (VACV) infection have defined molecular characteristics of poxvirus biology. However, little is known about the monkeypox (mpox) virus (MPXV) in humans, which has a different disease manifestation de

Externí odkaz: https://doaj.org/article/b2ed5b05f7224f76b575fd05ca07bceb

Quantitative proteomics defines mechanisms of antiviral defence and cell death during modified vaccinia Ankara infection

Autor: Jonas D. Albarnaz, Joanne Kite, Marisa Oliveira, Hanqi Li, Ying Di, Maria H. Christensen, Joao A. Paulo, Robin Antrobus, Steven P. Gygi, Florian I. Schmidt, Edward L. Huttlin, Geoffrey L. Smith, Michael P. Weekes

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)

Abstract Modified vaccinia Ankara (MVA) virus does not replicate in human cells and is the vaccine deployed to curb the current outbreak of mpox. Here, we conduct a multiplexed proteomic analysis to quantify >9000 cellular and ~80% of viral proteins

Externí odkaz: https://doaj.org/article/c5eb5a2f13b44fa3a6446d25a2216f50

Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium

Autor: Jan N Hansen, Fabian Kaiser, Christina Klausen, Birthe Stüven, Raymond Chong, Wolfgang Bönigk, David U Mick, Andreas Möglich, Nathalie Jurisch-Yaksi, Florian I Schmidt, Dagmar Wachten

Publikováno v: eLife, Vol 9 (2020)

Compartmentalization of cellular signaling forms the molecular basis of cellular behavior. The primary cilium constitutes a subcellular compartment that orchestrates signal transduction independent from the cell body. Ciliary dysfunction causes sever

Externí odkaz: https://doaj.org/article/586315dafc5549b09a3829c5629e34cd

Inhibition of cellular RNA methyltransferase abrogates influenza virus capping and replication

Autor: Yuta Tsukamoto, Takahiro Hiono, Shintaro Yamada, Keita Matsuno, Aileen Faist, Tobias Claff, Jianyu Hou, Vigneshwaran Namasivayam, Anja vom Hemdt, Satoko Sugimoto, Jin Ying Ng, Maria H. Christensen, Yonas M. Tesfamariam, Steven Wolter, Stefan Juranek, Thomas Zillinger, Stefan Bauer, Takatsugu Hirokawa, Florian I. Schmidt, Georg Kochs, Masayuki Shimojima, Yi-Shuian Huang, Andreas Pichlmair, Beate M. Kümmerer, Yoshihiro Sakoda, Martin Schlee, Linda Brunotte, Christa E. Müller, Manabu Igarashi, Hiroki Kato

Publikováno v: Tsukamoto, Y, Hiono, T, Yamada, S, Matsuno, K, Faist, A, Claff, T, Hou, J, Namasivayam, V, vom Hemdt, A, Sugimoto, S, Ng, J Y, Christensen, M H, Tesfamariam, Y M, Wolter, S, Juranek, S, Zillinger, T, Bauer, S, Hirokawa, T, Schmidt, F I, Kochs, G, Shimojima, M, Huang, Y S, Pichlmair, A, Kümmerer, B M, Sakoda, Y, Schlee, M, Brunotte, L, Müller, C E, Igarashi, M & Kato, H 2023, ' Inhibition of cellular RNA methyltransferase abrogates influenza virus capping and replication ', Science, vol. 379, no. 6632, pp. 586-591 . https://doi.org/10.1126/science.add0875

Orthomyxo- and bunyaviruses steal the 5′ cap portion of host RNAs to prime their own transcription in a process called “cap snatching.” We report that RNA modification of the cap portion by host 2′-O-ribose methyltransferase 1 (MTr1) is essen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22ca613036512c6c980ca13774bda357
https://pure.au.dk/portal/da/publications/inhibition-of-cellular-rna-methyltransferase-abrogates-influenza-virus-capping-and-replication(e57afff0-1209-4d3e-92f4-f592420aad55).html

Mitochondrial damage activates the NLRP10 inflammasome

Autor: Tomasz Próchnicki, Matilde B. Vasconcelos, Kim S. Robinson, Matthew S. J. Mangan, Dennis De Graaf, Kateryna Shkarina, Marta Lovotti, Lena Standke, Romina Kaiser, Rainer Stahl, Fraser G. Duthie, Maximilian Rothe, Kateryna Antonova, Lea-Marie Jenster, Zhi Heng Lau, Sarah Rösing, Nora Mirza, Clarissa Gottschild, Dagmar Wachten, Claudia Günther, Thomas A. Kufer, Florian I. Schmidt, Franklin L. Zhong, Eicke Latz

Publikováno v: Nature immunology 24(4), 595-603 (2023). doi:10.1038/s41590-023-01451-y

Upon detecting pathogens or cell stress, several NOD-like receptors (NLRs) form inflammasome complexes with the adapter ASC and caspase-1, inducing gasdermin D (GSDMD)-dependent cell death and maturation and release of IL-1β and IL-18. The triggers

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::951cef744dde927be85ce561d1cb4398