Zobrazeno 1 - 10
of 238
pro vyhledávání: '"Florian, Hollfelder"'
Autor:
Ana L. J. L. Ribeiro, Patricia Pérez-Arnaiz, Mercedes Sánchez-Costa, Lara Pérez, Marcos Almendros, Liisa van Vliet, Fabrice Gielen, Jesmine Lim, Simon Charnock, Florian Hollfelder, J. Eduardo González-Pastor, José Berenguer, Aurelio Hidalgo
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-14 (2024)
Abstract Background In vitro expression involves the utilization of the cellular transcription and translation machinery in an acellular context to produce one or more proteins of interest and has found widespread application in synthetic biology and
Externí odkaz:
https://doaj.org/article/fabf2155b3e74a7ca7d5bcf1d40d2296
Autor:
Joachim De Jonghe, Tomasz S. Kaminski, David B. Morse, Marcin Tabaka, Anna L. Ellermann, Timo N. Kohler, Gianluca Amadei, Charlotte E. Handford, Gregory M. Findlay, Magdalena Zernicka-Goetz, Sarah A. Teichmann, Florian Hollfelder
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-18 (2023)
Abstract Droplet microfluidic methods have massively increased the throughput of single-cell sequencing campaigns. The benefit of scale-up is, however, accompanied by increased background noise when processing challenging samples and the overall RNA
Externí odkaz:
https://doaj.org/article/2dff37ca2c9b446281d0fc46cf2e490a
Autor:
Timo N. Kohler, Joachim De Jonghe, Anna L. Ellermann, Ayaka Yanagida, Michael Herger, Erin M. Slatery, Antonia Weberling, Clara Munger, Katrin Fischer, Carla Mulas, Alex Winkel, Connor Ross, Sophie Bergmann, Kristian Franze, Kevin Chalut, Jennifer Nichols, Thorsten E. Boroviak, Florian Hollfelder
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-19 (2023)
Abstract Biomechanical cues are instrumental in guiding embryonic development and cell differentiation. Understanding how these physical stimuli translate into transcriptional programs will provide insight into mechanisms underlying mammalian pre-imp
Externí odkaz:
https://doaj.org/article/19d37a4e6ae24ca8b14f83a6a064a669
Autor:
Juan Carlos Yam-Puc, Zhaleh Hosseini, Emily C. Horner, Pehuén Pereyra Gerber, Nonantzin Beristain-Covarrubias, Robert Hughes, Aleksei Lulla, Maria Rust, Rebecca Boston, Magda Ali, Katrin Fischer, Edward Simmons-Rosello, Martin O’Reilly, Harry Robson, Lucy H. Booth, Lakmini Kahanawita, Andrea Correa-Noguera, David Favara, Lourdes Ceron-Gutierrez, Baerbel Keller, Andrew Craxton, Georgina S. F. Anderson, Xiao-Ming Sun, Anne Elmer, Caroline Saunders, Areti Bermperi, Sherly Jose, Nathalie Kingston, Thomas E. Mulroney, Lucia P. G. Piñon, CITIID-NIHR COVID−19 BioResource Collaboration, Michael A. Chapman, Sofia Grigoriadou, Marion MacFarlane, Anne E. Willis, Kiran R. Patil, Sarah Spencer, Emily Staples, Klaus Warnatz, Matthew S. Buckland, Florian Hollfelder, Marko Hyvönen, Rainer Döffinger, Christine Parkinson, Sara Lear, Nicholas J. Matheson, James E. D. Thaventhiran
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Abstract Age-associated B cells (ABC) accumulate with age and in individuals with different immunological disorders, including cancer patients treated with immune checkpoint blockade and those with inborn errors of immunity. Here, we investigate whet
Externí odkaz:
https://doaj.org/article/6ba7520f8a864e5db48cb0eaab277223
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture
Autor:
Philipp Knyphausen, Mariana Rangel Pereira, Paul Brear, Marko Hyvönen, Lutz Jermutus, Florian Hollfelder
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-13 (2023)
Custom proteases find applications as therapeutics, in research and in biotechnological applications. Here, the authors establish a protease selection system based on bacterial alpha-2-macroglobulin protease inhibitors and evolve staphylococcal prote
Externí odkaz:
https://doaj.org/article/4a5f0a823f2043db90aef3f41550275c
Vector redesign and in‐droplet cell‐growth improves enrichment and recovery in live Escherichia coli
Autor:
Bernard D. G. Eenink, Tomasz S. Kaminski, Erich Bornberg‐Bauer, Joachim Jose, Florian Hollfelder, Bert vanLoo
Publikováno v:
Microbial Biotechnology, Vol 15, Iss 11, Pp 2845-2853 (2022)
Abstract Directed evolution (DE) is a widely used method for improving the function of biomolecules via multiple rounds of mutation and selection. Microfluidic droplets have emerged as an important means to screen the large libraries needed for DE, b
Externí odkaz:
https://doaj.org/article/c4f747cc27c4473aa52f5ceedc1452df
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102333- (2023)
Summary: Within the peri-portal region of the adult liver, portal fibroblasts exist in close proximity to epithelial ductal/cholangiocyte cells. However, the cellular interactions between them are poorly understood. Here, we provide two co-culture te
Externí odkaz:
https://doaj.org/article/b18dbcef0c454e0f98bc0ad952eeeecc
Autor:
Remkes A. Scheele, Laurens H. Lindenburg, Maya Petek, Markus Schober, Kevin N. Dalby, Florian Hollfelder
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Here, the authors use a droplet-based screen for phosphate transfer catalysis, testing variants of the human protein kinase MKK1 for its ability to activate its downstream target ERK2. Data reveal a flexible motif in the MKK1 docking domain that prom
Externí odkaz:
https://doaj.org/article/5d041683bf7f4218bf7e710dc897fb55
Autor:
Andrea Schenkmayerova, Gaspar P. Pinto, Martin Toul, Martin Marek, Lenka Hernychova, Joan Planas-Iglesias, Veronika Daniel Liskova, Daniel Pluskal, Michal Vasina, Stephane Emond, Mark Dörr, Radka Chaloupkova, David Bednar, Zbynek Prokop, Florian Hollfelder, Uwe T. Bornscheuer, Jiri Damborsky
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Directed evolution commonly relies on point mutations but InDels frequently occur in evolution. Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer
Externí odkaz:
https://doaj.org/article/3c18b7a5373d42f0b49ea5eb1b1dcbad
Autor:
Paul Jannis Zurek, Philipp Knyphausen, Katharina Neufeld, Ahir Pushpanath, Florian Hollfelder
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
The success of protein evolution is dependent on the sequence context mutations are introduced into. Here the authors present UMIC-seq that allows consensus generation for closely related genes by using unique molecular identifiers linked to gene var
Externí odkaz:
https://doaj.org/article/e4783caa57744ab09ff2474ed8ee0e3c