Label-Free Homogeneous microRNA Detection in Cell Culture Medium Based on Graphene Oxide and Specific Fluorescence Quenching

Autor: Florentin R. Nitu, Lorand Savu, Sorin Muraru, Ioan Stoian, Mariana Ionită

Publikováno v: Nanomaterials, Vol 11, Iss 2, p 368 (2021)

Label-free homogeneous optical detection of low concentration of oligonucleotides using graphene oxide in complex solutions containing proteins remains difficult. We used a colloidal graphene oxide (GO) as a fluorescent probe quencher to detect micro

Externí odkaz: https://doaj.org/article/c6b1a6887d8645069e855579b1fc4746

Oligonucleotide Detection and Optical Measurement with Graphene Oxide in the Presence of Bovine Serum Albumin Enabled by Use of Surfactants and Salts

Autor: Florentin R. Nitu, Mariana Ionita, Jorge S. Burns

Publikováno v: Coatings
Volume 10
Issue 4
Coatings, Vol 10, Iss 420, p 420 (2020)

As graphene oxide-based oligonucleotide biosensors improve, there is a growing need to explore their ability to retain high sensitivity for low target concentrations in the context of biological fluids. Therefore, we innovatively combined assay milie

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08c2720962f3adc328ac887431a3c8c6
https://doi.org/10.3390/coatings10040420

High-Throughput Screens to Discover Small-Molecule Modulators of Ryanodine Receptor Calcium Release Channels

Autor: Benjamin D. Grant, Florentin R. Nitu, Maram M. Essawy, Robyn T. Rebbeck, David D. Thomas, Gregory D. Gillispie, Razvan L. Cornea, Donald M. Bers

Publikováno v: Rebbeck, RT; Essawy, MM; Nitu, FR; Grant, BD; Gillispie, GD; Thomas, DD; et al.(2017). High-Throughput Screens to Discover Small-Molecule Modulators of Ryanodine Receptor Calcium Release Channels. SLAS DISCOVERY, 22(2), 176-186. doi: 10.1177/1087057116674312. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/9vz1m8kk
SLAS discovery : advancing life sciences R & D, vol 22, iss 2

Using time-resolved fluorescence resonance energy transfer (FRET), we have developed and validated the first high-throughput screening (HTS) method to discover compounds that modulate an intracellular Ca2+ channel, the ryanodine receptor (RyR), for t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea5c2989754d7c43f4ec872e3d82b346
http://www.escholarship.org/uc/item/9vz1m8kk

Novel CPVT-Associated Calmodulin Mutation in CALM3 (CALM3-A103V) Activates Arrhythmogenic Ca Waves and Sparks

Autor: Nieves Gomez-Hurtado, Florentin R. Nitu, Michael J. Ackerman, Jennifer Sun, Bjorn C. Knollmann, Melissa L. Calvert, Dmytro O. Kryshtal, Nicole J. Boczek, Walter J. Chazin, David J. Tester, Christopher N. Johnson, Razvan L. Cornea

Publikováno v: Circulation: Arrhythmia and Electrophysiology. 9

Background— Calmodulin (CaM) mutations are associated with severe forms of long QT syndrome and catecholaminergic polymorphic ventricular tachycardia (CPVT). CaM mutations are found in 13% of genotype-negative long QT syndrome patients, but the pre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d92416d665fe1e23073b6038b7ce3f80
https://doi.org/10.1161/circep.116.004161

FRET-Based Mapping of Calmodulin within the Ryanodine Receptor

Autor: Donald M. Bers, Robyn T. Rebbeck, David D. Thomas, Florentin R. Nitu, Montserrat Samsó, Razvan L. Cornea, Bengt Svensson

Publikováno v: Biophysical Journal. 110(3)

Using time-resolved fluorescence resonance energy transfer (TR-FRET) and multi-site-directed labeling of calmodulin (CaM) and FKBP12.6, we further resolve the structural basis underlying CaM regulation of skeletal and cardiac ryanodine receptor isofo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8713a86310c89bdaaf93f170701a024