Genome-wide mapping of individual replication fork velocities using nanopore sequencing

Autor: Bertrand Theulot, Laurent Lacroix, Jean-Michel Arbona, Gael A. Millot, Etienne Jean, Corinne Cruaud, Jade Pellet, Florence Proux, Magali Hennion, Stefan Engelen, Arnaud Lemainque, Benjamin Audit, Olivier Hyrien, Benoît Le Tallec

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)

Theulot et al. introduce NanoForkSpeed, a nanopore sequencing-based method to map individual replication fork velocities on entire genomes. NFS shows that fork speed is uniform across yeast chromosomes except for a marked slowdown at pausing sites.

Externí odkaz: https://doaj.org/article/b8e2f80d57284f0b8386e98104ca5aad

Transcription Factors Modulate c-Fos Transcriptional Bursts

Autor: Adrien Senecal, Brian Munsky, Florence Proux, Nathalie Ly, Floriane E. Braye, Christophe Zimmer, Florian Mueller, Xavier Darzacq

Publikováno v: Cell Reports, Vol 8, Iss 1, Pp 75-83 (2014)

Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chromatin environment is known to influence the bursting behavior on long timescales, the impact of transcription factors (TFs)—especially in rapidly indu

Externí odkaz: https://doaj.org/article/ee02740268774e7eae322be169ec8d2c

FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing

Autor: Elizaveta A. Novikova, Corinne Cruaud, Arnaud Lemainque, Benoît Le Tallec, Stefan Engelen, Laurent Lacroix, Olivier Hyrien, Bertrand Theulot, Benjamin Audit, Magali Hennion, Xia Wu, Jean-Michel Arbona, Florence Proux

Publikováno v: Genome Biology
Genome Biology, 2020, 21, ⟨10.1186/s13059-020-02013-3⟩
Genome Biology, BioMed Central, 2020, 21 (1), ⟨10.1186/s13059-020-02013-3⟩
Genome Biology, BioMed Central, 2020, 21, pp.125. ⟨10.1186/s13059-020-02013-3⟩
Genome Biology, Vol 21, Iss 1, Pp 1-25 (2020)
Genome Biology, BioMed Central, 2020, 21, ⟨10.1186/s13059-020-02013-3⟩
Genome Biology, 2020, 21, pp.125. ⟨10.1186/s13059-020-02013-3⟩

Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200 nucleotide resolution. By quantifying BrdU inc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d991b712969cd41f141ed758685a89ff
https://hal.science/hal-03055281/file/s13059-020-02013-3.pdf

The crystal structure of Rv2991 from Mycobacterium tuberculosis: An F

Autor: Stefano, Benini, Ahmed, Haouz, Florence, Proux, Pedro, Alzari, Keith, Wilson

Publikováno v: Journal of structural biology. 206(2)

The crystal structure of the conserved hypothetical protein Rv2991 from Mycobacterium tuberculosis has been solved by SAD using seleno-methionine substituted protein. The dimeric biological assembly and the sequence and fold conservation are typical

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::62c765c5bc367ff3d08e416f39bce3fa
https://pubmed.ncbi.nlm.nih.gov/30890426

Transcription Factors Modulate c-Fos Transcriptional Bursts

Autor: Brian Munsky, Floriane E. Braye, Florence Proux, Xavier Darzacq, Christophe Zimmer, Nathalie Ly, Florian Mueller, Adrien Senecal

Publikováno v: Cell Reports
Cell Reports, 2014, 8 (1), pp.75-83. ⟨10.1016/j.celrep.2014.05.053⟩
Cell Reports, Vol 8, Iss 1, Pp 75-83 (2014)
Cell Reports, Elsevier Inc, 2014, 8 (1), pp.75-83. ⟨10.1016/j.celrep.2014.05.053⟩
Cell reports, vol 8, iss 1
Cell reports

International audience; Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chro-matin environment is known to influence the bursting behavior on long timescales, the impact of transcription factors (TFs)—e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73115335dbf97f3a2aeca08f6acf4011