Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Flavio Henrique Moreira Souza"'
Autor:
Sandra Mara Naressi Scapin, Flavio Henrique Moreira Souza, Leticia Maria Zanphorlin, Thamyres Silva de Almeida, Youssef Bacila Sade, Alexander Machado Cardoso, Guilherme Luiz Pinheiro, Mario Tyago Murakami
Publikováno v:
PLoS ONE, Vol 12, Iss 4, p e0176550 (2017)
Cellulose synthesis in bacteria is a complex process involving the concerted action of several enzymes whose genes are often organized in operons. This process influences many fundamental physiological aspects such as bacteria and host interaction, b
Externí odkaz:
https://doaj.org/article/3604ee462e754346ac54fbeaad11cfce
Autor:
Mariana Abrahão Bueno de Morais, Jessica B. L. Correa, Mário T. Murakami, Camila R. Santos, Mariane Noronha Domingues, Renan A. S. Pirolla, Carla Cristina Polo, Gabriela Felix Persinoti, Flavio Henrique Moreira Souza
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Frontiers in Bioengineering and Biotechnology
Frontiers in Bioengineering and Biotechnology
The glycoside hydrolase family 39 (GH39) is a functionally expanding family with limited understanding about the molecular basis for substrate specificity and extremophilicity. In this work, we demonstrate the key role of the positive-subsite region
Autor:
Mário T. Murakami, Fabio C. Gozzo, Camila R. Santos, Letícia Maria Zanphorlin, Plínio Salmazo Vieira, Mariane Noronha Domingues, Renan A. S. Pirolla, Rodrigo V. Honorato, Paulo S. Oliveira, Mariana Abrahão Bueno de Morais, Flavio Henrique Moreira Souza
Publikováno v:
Journal of Biological Chemistry. 293:13636-13649
The classical microbial strategy for depolymerization of β-mannan polysaccharides involves the synergistic action of at least two enzymes, endo-1,4-β-mannanases and β-mannosidases. In this work, we describe the first exo-β-mannanase from the GH2
Autor:
Gabriela L. Berto, Fernando Segato, Fabio M. Squina, Igor Polikarpov, Dyoni Matias de Oliveira, Flavio Henrique Moreira Souza, André Damasio, Mário T. Murakami, Bruno Vilas Boas Dias, Ana Paula Citadini
Publikováno v:
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Universidade de São Paulo (USP)
instacron:USP
The cellulases from Glycoside Hydrolyses family 12 (GH12) play an important role in cellulose degradation and plant cell wall deconstruction being widely used in a number of bioindustrial processes. Aiming to contribute toward better comprehension of
Autor:
Alex C. Gazolla, Flavio Henrique Moreira Souza, Felipe Resende Nóbrega, Elaine Crespim, Amanda Silva de Sousa, Fernanda Lopes de Figueiredo, Carla Botelho Machado, José A. Diogo, Letícia Maria Zanphorlin, Mário T. Murakami, Roberto Ruller, Vivian Helena Pellizari
Publikováno v:
International Journal of Biological Macromolecules. 82:375-380
A novel GH1 β-glucosidase (EaBgl1A) from a bacterium isolated from Antarctica soil samples was recombinantly overexpressed in Escherichia coli cells and characterized. The enzyme showed unusual pH dependence with maximum activity at neutral pH and r
Autor:
Fabio C. Gozzo, Priscila Oliveira de Giuseppe, Flavio Henrique Moreira Souza, Lucas Miranda Fonseca, Rodrigo V. Honorato, Mariana Abrahão Bueno de Morais, Renan A. S. Pirolla, Celisa Caldana Costa Tonoli, Vanesa Peixoto de Matos Martins, Paulo S. Oliveira, Fernanda Büchli, Camila R. Santos, Mariane Noronha Domingues, Letícia Maria Zanphorlin, Mário T. Murakami
Publikováno v:
Biotechnology for Biofuels
Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-19 (2018)
Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-19 (2018)
Background Arabinoxylan is an abundant polysaccharide in industrially relevant biomasses such as sugarcane, corn stover and grasses. However, the arabinofuranosyl di-substitutions that decorate the xylan backbone are recalcitrant to most known arabin
Autor:
D. C. Masui, João Atílio Jorge, Ana Lucia Ribeiro Latorre Zimbardi, Tony Marcio da Silva, Flavio Henrique Moreira Souza, Luana Parras Meleiro, Rosa dos Prazeres Melo Furriel
Publikováno v:
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Universidade de São Paulo (USP)
instacron:USP
Humicola insolens produced a new β-glucosidase (BglHi2) under solid-state fermentation. The purified enzyme showed apparent molecular masses of 116 kDa (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and 404 kDa (gel-filtration), suggest
Autor:
Rodrigo Furriel Inocentes, Richard J. Ward, Rosa dos Prazeres Melo Furriel, Flavio Henrique Moreira Souza, João Atílio Jorge
Publikováno v:
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Universidade de São Paulo (USP)
instacron:USP
β-Glucosidases activated by glucose and xylose are uncommon yet intriguing enzymes that may enhance cellulose saccharification efficiency, and are of interest for application in bioethanol production processes. The molecular mechanisms of activation
Autor:
Monica Stropa Ferreira Nozawa, Ana Lucia Ribeiro Latorre Zimbardi, João Atílio Jorge, Luana Parras Meleiro, Flavio Henrique Moreira Souza, Rosa dos Prazeres Melo Furriel, Cesar Sehn, Luis Henrique Souza Guimarães, D. C. Masui
Publikováno v:
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
International Journal of Molecular Sciences, Vol 14, Iss 2, Pp 2875-2902 (2013)
International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 14; Issue 2; Pages: 2875-2902
Universidade de São Paulo (USP)
instacron:USP
International Journal of Molecular Sciences, Vol 14, Iss 2, Pp 2875-2902 (2013)
International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 14; Issue 2; Pages: 2875-2902
Efficient, low-cost enzymatic hydrolysis of lignocellulosic residues is essential for cost-effective production of bioethanol. The production of β-glucosidase, β-xylosidase and xylanase by Colletotrichum graminicola was optimized using Response Sur
Autor:
Flavio Henrique Moreira Souza, D. C. Masui, José César Rosa, Francisco A. Leone, João Atílio Jorge, Rosa dos Prazeres Melo Furriel, César Vanderlei Nascimento
Publikováno v:
Process Biochemistry. 45:272-278
A mycelial β-glucosidase from the thermophilic mold Humicola insolens was purified and biochemically characterized. The enzyme showed carbohydrate content of 21% and apparent molecular mass of 94 kDa, as estimated by gel filtration. Sodium dodecyl s