ENDO-Pore: high-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing

Autor: Fiona M. Diffin, Mark D. Szczelkun, Kincaid W A Ingram, Stephen Cross, Oscar E Torres Montaguth, Laura Lee

Publikováno v: Nucleic Acids Research
Torres Montaguth, O E, Cross, S J, Ingram, K W A, Lee, L, Diffin, F M & Szczelkun, M D 2021, ' ENDO-Pore : high-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing ', Nucleic Acids Research, vol. 49, no. 20, gkab727 . https://doi.org/10.1093/nar/gkab727

Mapping the precise position of DNA cleavage events plays a key role in determining the mechanism and function of endonucleases. ENDO-Pore is a high-throughput nanopore-based method that allows the time resolved mapping single molecule DNA cleavage e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39c03393ef4a145a8863d34aa77f4f72
https://pubmed.ncbi.nlm.nih.gov/34417616

5' modifications to CRISPR-Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage

Autor: Grace E L Mullally, Kara van Aelst, Fiona M. Diffin, Virginijus Siksnys, Mohsin M. Naqvi, Mark D. Szczelkun, Tautvydas Karvelis, Giedrius Gasiunas

Publikováno v: Nucleic acids research, Oxford : Oxford University Press, 2020, vol. 48, iss. 12, p. 6811-6823
Mullally, G E L, Van Aelst, K, Mubarak Naqvi, M, Diffin, F M, Karvelis, T, Gasiunas, G, Siksnys, V & Szczelkun, M D 2020, ' 5′ modifications to CRISPR Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage ', Nucleic Acids Research . https://doi.org/10.1093/nar/gkaa477
Nucleic Acids Research

A key aim in exploiting CRISPR–Cas is gRNA engineering to introduce additional functionalities, ranging from individual nucleotide changes that increase efficiency of on-target binding to the inclusion of larger functional RNA aptamers or ribonucle

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c3714a1d69aef21584daee7a78cc1e4
https://repository.vu.lt/VU:ELABAPDB62158247&prefLang=en_US

Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes

Autor: Kara van Aelst, Christian Pernstich, Kayarat Saikrishnan, Mahesh Kumar Chand, Neha Nirwan, Manasi Kulkarni, Mark D. Szczelkun, Fiona M. Diffin

Publikováno v: Chand, M K, Nirwan, N, Diffin, F M, van Aelst, K, Kulkarni, M, Pernstich, C, Szczelkun, M D & Saikrishnan, K 2015, ' Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes ', Nature Chemical Biology, vol. 11, pp. 870-877 . https://doi.org/10.1038/nchembio.1926
Nature chemical biology

Endonucleolytic double-strand DNA break production requires separate strand cleavage events. Although catalytic mechanisms for simple dimeric endonucleases are available, there are many complex nuclease machines which are poorly understood in compari

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c15cb90b723d81554205d1ebf06b2ea
https://research-information.bris.ac.uk/ws/files/47930130/15402_2_supp_196134_nt8ftt_convrt.pdf

The Type ISP Restriction-Modification enzymes LlaBIII and LlaGI use a translocation-collision mechanism to cleave non-specific DNA distant from their recognition sites

Autor: Mark D. Szczelkun, Fiona M. Diffin, Kara van Aelst, Eva Šišáková

Publikováno v: Nucleic Acids Research

The Type ISP Restriction-Modification (RM) enzyme LlaBIII is encoded on plasmid pJW566 and can protect Lactococcus lactis strains against bacteriophage infections in milk fermentations. It is a single polypeptide RM enzyme comprising Mrr endonuclease

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f32552b2006a92ae26495aca9fc9bbd4
https://pubmed.ncbi.nlm.nih.gov/23222132

Biosynthesis and metabolism of pyrazole by Cucumis sativus: Enzymic cyclization and dehydrogenation of 1,3-diaminopropane

Autor: Eric G. Brown, Fiona M. Diffin

Publikováno v: Phytochemistry. 29:469-478

Both 1,3-diaminopropane and pyrazole stimulate synthesis of β-pyrazol-1-ylalanine in seedlings and excised shoots of cucumber. With either plant system, increasing the concentration of pyrazole raised β-pyrazol-1-ylalanine yield to a maximum beyond

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7807303a9a7cdf65b5dc7a6c5b7ee1d1
https://doi.org/10.1016/0031-9422(90)85099-2