Bacterial cell-free expression technology to in vitro systems engineering and optimization

Autor: Filippo Caschera

Publikováno v: Synthetic and Systems Biotechnology, Vol 2, Iss 2, Pp 97-104 (2017)

Cell-free expression system is a technology for the synthesis of proteins in vitro. The system is a platform for several bioengineering projects, e.g. cell-free metabolic engineering, evolutionary design of experiments, and synthetic minimal cell con

Externí odkaz: https://doaj.org/article/8ef2dedec88e48ab96478c8b9b501505

Automated discovery of novel drug formulations using predictive iterated high throughput experimentation.

Autor: Filippo Caschera, Gianluca Gazzola, Mark A Bedau, Carolina Bosch Moreno, Andrew Buchanan, James Cawse, Norman Packard, Martin M Hanczyc

Publikováno v: PLoS ONE, Vol 5, Iss 1, p e8546 (2010)

BACKGROUND: We consider the problem of optimizing a liposomal drug formulation: a complex chemical system with many components (e.g., elements of a lipid library) that interact nonlinearly and synergistically in ways that cannot be predicted from fir

Externí odkaz: https://doaj.org/article/a6f9e6277d594750b3245dc2fe80a98a

High-Throughput Optimization Cycle of a Cell-Free Ribosome Assembly and Protein Synthesis System

Autor: Norman H. Packard, Anne E. d’Aquino, Filippo Caschera, Ashty S. Karim, Michael C. Jewett, Gianluca Gazzola

Publikováno v: ACS Synthetic Biology. 7:2841-2853

Building variant ribosomes offers opportunities to reveal fundamental principles underlying ribosome biogenesis and to make ribosomes with altered properties. However, cell viability limits mutations that can be made to the ribosome. To address this

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::914be64c2fa28bee660c77fbedacd13c
https://doi.org/10.1021/acssynbio.8b00276

Semiconductor Nanoplatelets: A New Class of Ultrabright Fluorescent Probes for Cytometric and Imaging Applications

Autor: Chloé Grazon, Djamila Kechkeche, Filippo Caschera, Benoit Dubertret, Vincent Noireaux, Edgar Cao, Marie Laurence Baron Niel

Publikováno v: ACS Applied Materials & Interfaces
ACS Applied Materials & Interfaces, Washington, D.C. : American Chemical Society, 2018, 10 (29), pp.24739-24749. ⟨10.1021/acsami.8b07143⟩

Fluorescent semiconductor nanoplatelets (NPLs) are a new generation of fluorescent probes. NPLs are colloidal two-dimensional materials that exhibit several unique optical properties, including high brightness, photostability, and extinction coeffici

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4db8f28d10c599266e21493fc0f0e92
https://hal.archives-ouvertes.fr/hal-03366531

Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

Autor: Allen P. Liu, Filippo Caschera, Kenneth K. Y. Ho, Jin Woo Lee, Michael C. Jewett

A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35d6314d4c5324fcecce722e18311bc4
https://europepmc.org/articles/PMC4829377/