Handling of spurious sequences affects the outcome of high-throughput 16S rRNA gene amplicon profiling.

Autor: Reitmeier S; ZIEL Institute for Food & Health, Technical University of Munich, Freising, Germany.; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Hitch TCA; Functional Microbiome Research Group, RWTH University Hospital, Aachen, Germany., Treichel N; Functional Microbiome Research Group, RWTH University Hospital, Aachen, Germany., Fikas N; Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece., Hausmann B; Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna, Vienna, Austria.; Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria., Ramer-Tait AE; Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA., Neuhaus K; ZIEL Institute for Food & Health, Technical University of Munich, Freising, Germany., Berry D; Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna, Vienna, Austria.; Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria., Haller D; ZIEL Institute for Food & Health, Technical University of Munich, Freising, Germany.; Chair of Nutrition and Immunology, Technical University of Munich, Freising, Germany., Lagkouvardos I; ZIEL Institute for Food & Health, Technical University of Munich, Freising, Germany. ilias.lagkouvardos@hcmr.gr.; Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece. ilias.lagkouvardos@hcmr.gr., Clavel T; ZIEL Institute for Food & Health, Technical University of Munich, Freising, Germany. tclavel@ukaachen.de.; Functional Microbiome Research Group, RWTH University Hospital, Aachen, Germany. tclavel@ukaachen.de.

Publikováno v: ISME communications [ISME Commun] 2021 Jun 29; Vol. 1 (1), pp. 31. Date of Electronic Publication: 2021 Jun 29.