Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching

Autor: Fernando Muzzopappa, Johan Hummert, Michela Anfossi, Stanimir Asenov Tashev, Dirk-Peter Herten, Fabian Erdel

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)

It is currently debated how to reliably distinguish liquid–liquid phase separation (LLPS) from other mechanisms. Here the authors report model-free calibrated half-FRAP (MOCHA-FRAP) to probe the barrier at the condensate interface that is responsib

Externí odkaz: https://doaj.org/article/874443a150d04973b28034e0cceabdb3

The molecular origin of the OCP-dependent non-photochemical quenching mechanism in cyanobacteria

Autor: Nicoletta Liguori, Ivo van Stokkum, Fernando Muzzopappa, John Kennis, Diana Kirilovsky, Roberta Croce

Cyanobacteria were the first microorganisms that released oxygen into the atmosphere billions of years ago. To do it safely under intense sunlight, they developed strategies that prevent photooxidation in the photosynthetic membrane. One of these str

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5a6addc1940eb4e45fe5cce133018b81
https://doi.org/10.26434/chemrxiv-2022-nkdw6

A kaleidoscope of photosynthetic antenna proteins and their emerging roles

Autor: Rameez Arshad, Francesco Saccon, Pushan Bag, Avratanu Biswas, Claudio Calvaruso, Ahmad Farhan Bhatti, Steffen Grebe, Vincenzo Mascoli, Moontaha Mahbub, Fernando Muzzopappa, Alexandros Polyzois, Christo Schiphorst, Mirella Sorrentino, Simona Streckaité, Herbert van Amerongen, Eva-Mari Aro, Roberto Bassi, Egbert J Boekema, Roberta Croce, Jan Dekker, Rienk van Grondelle, Stefan Jansson, Diana Kirilovsky, Roman Kouřil, Sylvie Michel, Conrad W Mullineaux, Klára Panzarová, Bruno Robert, Alexander V Ruban, Ivo van Stokkum, Emilie Wientjes, Claudia Büchel

Publikováno v: Plant physiology, 189(3), 1204-1219. American Society of Plant Biologists
Plant Physiology 189 (2022) 3
Plant Physiology, 189(3), 1204-1219
Arshad, R, Saccon, F, Bag, P, Biswas, A, Calvaruso, C, Bhatti, A F, Grebe, S, Mascoli, V, Mahbub, M, Muzzopappa, F, Polyzois, A, Schiphorst, C, Sorrentino, M, Streckaité, S, van Amerongen, H, Aro, E M, Bassi, R, Boekema, E J, Croce, R, Dekker, J, van Grondelle, R, Jansson, S, Kirilovsky, D, Kouřil, R, Michel, S, Mullineaux, C W, Panzarová, K, Robert, B, Ruban, A V, van Stokkum, I, Wientjes, E & Büchel, C 2022, ' A kaleidoscope of photosynthetic antenna proteins and their emerging roles ', Plant physiology, vol. 189, no. 3, pp. 1204-1219 . https://doi.org/10.1093/plphys/kiac175
Plant Physiology, 189(3), 1204-1219. AMER SOC PLANT BIOLOGISTS

Photosynthetic light-harvesting antennae are pigment-binding proteins that perform one of the most fundamental tasks on Earth, capturing light and transferring energy that enables life in our biosphere. Adaptation to different light environments led

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bab9c98219df64c7aaf91320947703fe
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-194031

Structural dynamics in the C terminal domain homolog of orange carotenoid protein reveals residues critical for carotenoid uptake

Autor: Fabian Glaser, Dvir Harris, Fernando Muzzopappa, Adjélé Wilson, Diana Kirilovsky, Noam Adir

Publikováno v: Biochimica biophysica acta (BBA)-Bioenergetics
Biochimica biophysica acta (BBA)-Bioenergetics, 2020, pp.148214. ⟨10.1016/j.bbabio.2020.148214⟩
Biochimica biophysica acta (BBA)-Bioenergetics, Elsevier, 2020, pp.148214. ⟨10.1016/j.bbabio.2020.148214⟩

The structural features enabling carotenoid translocation between molecular entities in nature is poorly understood. Here, we present the three-dimensional X-ray structure of an expanded oligomeric state of the C-terminal domain homolog (CTDH) of the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8beac59baca243ada00aeae8fb8d3f04
https://hal.science/hal-02570032/document

Paralogs of the C-Terminal Domain of the Cyanobacterial Orange Carotenoid Protein Are Carotenoid Donors to Helical Carotenoid Proteins

Autor: Sandrine Cot, Cédric Montigny, Adjélé Wilson, Céline Bourcier de Carbon, Fernando Muzzopappa, François Perreau, Vinosa Yogarajah, Diana Kirilovsky

Publikováno v: Plant Physiology

The photoactive Orange Carotenoid Protein (OCP) photoprotects cyanobacteria cells by quenching singlet oxygen and excess excitation energy. Its N-terminal domain is the active part of the protein, and the C-terminal domain regulates the activity. Rec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::348a06e295d2aa9c9f2035eca798b72e
https://doi.org/10.1104/pp.17.01040