Novel Template Plasmids pCyaA’-Kan and pCyaA’-Cam for Generation of Unmarked Chromosomal cyaA’ Translational Fusion to T3SS Effectors in Salmonella

Autor: Paulina A. Fernández, Marcela Zabner, Jaime Ortega, Constanza Morgado, Fernando Amaya, Gabriel Vera, Carolina Rubilar, Beatriz Salas, Víctor Cuevas, Camila Valenzuela, Fernando Baisón-Olmo, Sergio A. Álvarez, Carlos A. Santiviago

Publikováno v: Microorganisms, Vol 9, Iss 3, p 475 (2021)

The type III secretion systems (T3SS) encoded in pathogenicity islands SPI-1 and SPI-2 are key virulence factors of Salmonella. These systems translocate proteins known as effectors into eukaryotic cells during infection. To characterize the function

Externí odkaz: https://doaj.org/article/47c25183ae5c435da6e5eb822d4c6bff

Novel Template Plasmids pCyaA’-Kan and pCyaA’-Cam for Generation of Unmarked Chromosomal cyaA’ Translational Fusion to T3SS Effectors in Salmonella

Autor: Jaime H. Ortega, Beatriz Salas, Sergio A. Álvarez, Fernando Amaya, Carlos A. Santiviago, Camila Valenzuela, Paulina A Fernández, Constanza Morgado, Carolina Rubilar, Víctor Cuevas, Fernando Baisón-Olmo, Gabriel Vera, Marcela Zabner

Publikováno v: Microorganisms, Vol 9, Iss 475, p 475 (2021)
Microorganisms
Volume 9
Issue 3

The type III secretion systems (T3SS) encoded in pathogenicity islands SPI-1 and SPI-2 are key virulence factors of Salmonella. These systems translocate proteins known as effectors into eukaryotic cells during infection. To characterize the function

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::297115d7ee0423757ab786fc3026bed6
https://www.mdpi.com/2076-2607/9/3/475

Generation and use of site-directed chromosomal cyaA' translational fusions in Salmonella enterica

Autor: Francisco, Ramos-Morales, Elena, Cardenal-Muñoz, Mar, Cordero-Alba, Fernando, Baisón-Olmo

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1225

CyaA from Bordetella pertussis is a calmodulin-dependent adenylate cyclase. Fusions to the catalytic domain of CyaA (CyaA') are useful tools to detect translocation of type III secretion system effectors from gram-negative pathogens like Salmonella e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ea257e5a8267919023d0b25c0838e116
https://pubmed.ncbi.nlm.nih.gov/25253250

Tubulin Folding Cofactor TBCB is a Target of the Salmonella Effector Protein SseK1

Autor: Juan Luis Araujo-Garrido, Francisco Romero, Fernando Baisón-Olmo, Francisco Ramos-Morales, Joaquín Bernal-Bayard

Publikováno v: idUS: Depósito de Investigación de la Universidad de Sevilla
Universidad de Sevilla (US)
International Journal of Molecular Sciences
idUS. Depósito de Investigación de la Universidad de Sevilla
instname
International Journal of Molecular Sciences, Vol 21, Iss 3193, p 3193 (2020)
Volume 21
Issue 9

Salmonella enterica serovar Typhimurium is a human and animal pathogen that uses type III secretion system effectors to manipulate the host cell and fulfill infection. SseK1 is a Salmonella effector with glycosyltransferase activity. We carried out a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01f2159ab357f4eb6b55001683bfa57f