Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Fernan Federici"'
Autor:
Paul K Grant, Neil Dalchau, James R Brown, Fernan Federici, Timothy J Rudge, Boyan Yordanov, Om Patange, Andrew Phillips, Jim Haseloff
Publikováno v:
Molecular Systems Biology, Vol 12, Iss 1, Pp 1-13 (2016)
Abstract Bidirectional intercellular signaling is an essential feature of multicellular organisms, and the engineering of complex biological systems will require multiple pathways for intercellular signaling with minimal crosstalk. Natural quorum‐s
Externí odkaz:
https://doaj.org/article/4ee467c47465413882c5711a1af8082d
The Golden Gate technique allows the assembly of genetic sequences from libraries of standardized basic components, which are cleaved from their donor vectors and concatenated in the acceptor vector in a defined order. This reaction is subsequently t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ef86b936b437624072b19d3693d1e2d
https://doi.org/10.17504/protocols.io.b6zirf4e
https://doi.org/10.17504/protocols.io.b6zirf4e
Autor:
Fernando Guzman-Chavez, Anibal Arce, Abhinav Adhikari, Sandra Vadhin, Jose Antonio Pedroza-Garcia, Chiara Gandini, Jim W. Ajioka, Jenny Molloy, Sobeida Sanchez-Nieto, Jeffrey D. Varner, Fernan Federici, Jim Haseloff
Publikováno v:
ACS synthetic biology. 11(3)
Cell-free systems for gene expression have gained attention as platforms for the facile study of genetic circuits and as highly effective tools for teaching. Despite recent progress, the technology remains inaccessible for many in low- and middle-inc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56486c0791e59ab19ebd2defd83d29f7
https://pubmed.ncbi.nlm.nih.gov/35259873
https://pubmed.ncbi.nlm.nih.gov/35259873
Autor:
Keith J. M. Moore, Jeremy Cahill, Guy Aidelberg, Rachel Aronoff, Ali Bektaş, Daniela Bezdan, Daniel J. Butler, Sridar V. Chittur, Martin Codyre, Fernan Federici, Nathan A. Tanner, Scott W. Tighe, Randy True, Sarah B. Ware, Anne L. Wyllie, Evan E. Afshin, Andres Bendesky, Connie B. Chang, Richard Dela Rosa, Eran Elhaik, David Erickson, Andrew S. Goldsborough, George Grills, Kathrin Hadasch, Andrew Hayden, Seong-Young Her, Julie A. Karl, Chang Hee Kim, Alison J. Kriegel, Thomas Kunstman, Zeph Landau, Kevin Land, Bradley W. Langhorst, Ariel B. Lindner, Benjamin E. Mayer, Lee A. McLaughlin, Matthew T. McLaughlin, Jenny Molloy, Christopher Mozsary, Jerry L. Nadler, Melinee D'Silva, David Ng, David H. O'Connor, Jerry E. Ongerth, Olayinka Osuolale, Ana Pinharanda, Dennis Plenker, Ravi Ranjan, Michael Rosbash, Assaf Rotem, Jacob Segarra, Stephan Schürer, Scott Sherrill-Mix, Helena Solo-Gabriele, Shaina To, Merly C. Vogt, Albert D. Yu, Christopher E. Mason
Publikováno v:
J Biomol Tech
As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcc7049220c378abd20bf95f74cd84ab
https://pubmed.ncbi.nlm.nih.gov/35136384
https://pubmed.ncbi.nlm.nih.gov/35136384
Publikováno v:
IEEE Design & Test. :1-1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::59ffe3c75b59c514f957dba95ff9ce27
https://doi.org/10.1109/mdat.2023.3237942
https://doi.org/10.1109/mdat.2023.3237942
This protocol explains how to prepare dsDNA and ssRNA target positive control for LAMP and RT-LAMP reactions. Particularly it describes the pipeline for SARS-Cov-2 Nucleocapsid (N) gene positive control which is included in the ReClone collection. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::316d3335468ebf50f4d3763e604aa580
https://doi.org/10.17504/protocols.io.bsknncve
https://doi.org/10.17504/protocols.io.bsknncve
This protocol describes the preparation of reaction buffer for homemade LAMP / RT-LAMP reactions which involves the use of home-brewed BstLF and MMLV enzymes. It also contains the instructions to prepare the enzyme storage buffers. Home-brewed enzyme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f5c467a58f1f19f15d626f34aac564f0
https://doi.org/10.17504/protocols.io.bsehnbb6
https://doi.org/10.17504/protocols.io.bsehnbb6
This protocol collection specifies how to carry out LAMP or RT-LAMP (reverse transcription - Loop-mediated isothermal amplification) reactions with homemade reagents and home-brewed enzymes. It describes how to prepare all the solutions and buffers t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5a9a9b4747511393391a657fb25cebbc
https://doi.org/10.17504/protocols.io.bsejnbcn
https://doi.org/10.17504/protocols.io.bsejnbcn
This protocol describes how to perform LAMP / RT-LAMP reactions with home-made reagents, which means home-made buffers and positive controls, together with home-brewed BstLF and MMLV enzymes. Home-brewed enzymes information: BstLF polymerase: express
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ffd0a83aac30c37f802b6fb459f7364d
https://doi.org/10.17504/protocols.io.bsefnbbn
https://doi.org/10.17504/protocols.io.bsefnbbn
Autor:
Maira Rivera, Severine Cazaux, Ariel Cerda, Anibal Arce Medina, Isaac Núñez, Tamara Matute, Alex Brown, Javier Gasulla, Fernan Federici, Cesar A Ramirez-Sarmiento
This protocol has been optimized for the recombinant expression of a codon-optimized Bst-LF polymerase. The goal of this protocol was to eliminate the use of large volumes for dyalisis through the use of concentrators for buffer exchange before stora
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8a2c092ca6f3c0c2d5b5fc24769dccc0
https://doi.org/10.17504/protocols.io.bksrkwd6
https://doi.org/10.17504/protocols.io.bksrkwd6