Ca2+induces clustering of membrane proteins in the plasma membrane via electrostatic interactions

Autor: Nagaraj D. Halemani, Arne Schreiber, Reinhard Jahn, Thorsten Lang, Felipe E. Zilly, Luis Spitta, David Walrafen

Publikováno v: The EMBO Journal. 30:1209-1220

Membrane proteins and membrane lipids are frequently organized in submicron-sized domains within cellular membranes. Factors thought to be responsible for domain formation include lipid–lipid interactions, lipid–protein interactions and protein�

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6d0c62f8677d6f99c6581cc77b34a82b
https://doi.org/10.1038/emboj.2011.53

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

Autor: Horst Höbenreich, Sheng Wu, Sabrina Kille, Renate Lohmer, Felipe E. Zilly, Shreenath Prasad, Jérôme J.-P. Peyralans, Andreas Taglieber, Yosephine Gumulya, Daniel Kahakeaw, Jullien Drone, John Podtetenieff, J. Daniel Carballeira, Joaquin Sanchis, Manfred T. Reetz, Layla Fernández, Pankaj Soni

Publikováno v: Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology, Springer Verlag, 2008, 81, pp.387-397. ⟨10.1007/s00253-008-1678-9⟩

International audience; Saturation mutagenesis constitutes a powerful method in the directed evolution of enzymes. Traditional protocols of whole plasmid amplification such as Stratagene's QuikChange sometimes fail when the templates are difficult to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3eb1acbbd27e618aa874f76a63f5fc86
https://doi.org/10.1007/s00253-008-1678-9

A dual function for Munc-18 in exocytosis of PC12 cells

Autor: Reinhard Jahn, Thorsten Lang, Dieter Bruns, Felipe E. Zilly, Dagmar Schütz

Publikováno v: European Journal of Neuroscience. 21:2419-2432

Munc-18 interacts with the SNARE protein syntaxin and is supposed to influence transmitter release by controlling the formation of exocytosis-relevant SNARE complexes. Here, we used combined biochemical and physiological analyses to study the role of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::470b2ce12f8b7794ec9f7f858c65bc72
https://doi.org/10.1111/j.1460-9568.2005.04095.x

Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution

Autor: Sabrina Kille, Manfred T. Reetz, Juan Pablo Acevedo, Felipe E. Zilly

Publikováno v: Nature chemistry. 3(9)

A current challenge in synthetic organic chemistry is the development of methods that allow the regio- and stereoselective oxidative C-H activation of natural or synthetic compounds with formation of the corresponding alcohols. Cytochrome P450 enzyme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a64889d8a50e233e44682d1639f87799
https://pubmed.ncbi.nlm.nih.gov/21860465

A Flexible Syntaxin Solves the Mystery of the SNAREd Munc

Autor: Thorsten Lang, Felipe E. Zilly, Jakob B. Sørensen, Reinhard Jahn

Publikováno v: PLoS Biology, Vol 4, Iss 10, p e359 (2006)
PLoS Biology
PLoS Biology, Vol 4, Iss 10, p e330 (2006)

Munc18–1, a protein essential for regulated exocytosis in neurons and neuroendocrine cells, belongs to the family of Sec1/Munc18-like (SM) proteins. In vitro, Munc18–1 forms a tight complex with the SNARE syntaxin 1, in which syntaxin is stabiliz

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28b398bd9ce4bae87287c3d6f59e1da4
http://europepmc.org/articles/PMC1570501?pdf=render