Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Fei Li Gong"'
Publikováno v:
Gene. 509:149-153
This study aimed to explore the role of high mobility group [corrected] box 1 (HMGB1) and its receptor toll like receptor 4 (TLR4) on neutrophils in myocardial ischemia reperfusion (I/R) injury. We constructed TLR4-mutant (C3H/HeJ) and control (C3H/H
Publikováno v:
Cancer Genetics and Cytogenetics. 157:33-36
By means of flow cytometry, CD34+/CD38- hematopoietic stem cells (HSC) were collected from umbilical cord blood (UCB) of 10 healthy women at the time of delivery and cultivated in stem-cell culture media supplemented with cell growth stimulating fact
Autor:
Hua-Sheng, Ding, Jun, Yang, Fei-Li, Gong, Jian, Yang, Jia-Wang, Ding, Song, Li, Yu-Rong, Jiang
Publikováno v:
Gene. 509(1)
This study aimed to explore the role of high mobility group [corrected] box 1 (HMGB1) and its receptor toll like receptor 4 (TLR4) on neutrophils in myocardial ischemia reperfusion (I/R) injury. We constructed TLR4-mutant (C3H/HeJ) and control (C3H/H
Autor:
Zhen-hua, Zhang, Rong, Wang, Yong-jun, Tian, Lei, Li, Jian-bo, Xia, Jin-song, Gong, Meng-ji, Lu, Fei-li, Gong, Dong-liang, Yang
Publikováno v:
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology. 15(8)
To search for and verify some common B cell epitopes in the core proteins of woodchuck hepatitis virus and human hepatitis B virus.Monoclonal antibodies against both core proteins of woodchuck hepatitis virus (WHV) and human hepatitis B virus (HBV) w
Publikováno v:
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 22(4)
To study the regulatory effect of recombinant glutathione S-transferase (GST)-extracellular domain of murine B and T lymphocyte attenuator (mBTLAext) fusion protein GST-mBTLAext on the expression of B7 on murine dendritic cell (DC) line DC2.4.cDNA of
Publikováno v:
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 21(6)
To construct eukaryotic expression vector of human T-cell immunoglobulin mucin 3(TIM-3) and transfect mammalian cells to establish stable cell line.The whole coding region of TIM-3 was amplified by PCR and inserted into eukaryotic expression vector p
Autor:
Cai-e, Zhang, Jun-yan, Han, Hui-jun, Yang, Xiong-wen, Wu, Ya-fei, Huang, Zhi-hui, Liang, Fei-li, Gong
Publikováno v:
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 21(6)
To study the effect of HLA-G1 molecule expressed by an endothelial cell line (ECV304) on the cytotoxic activity of allogeneic NK cells.ECV304 cells were transfected with recombinant plasmid pcDNA3-HLA-G1 by the liposome transfection, and the expresse
Autor:
Qing, Li, Xiong-wen, Wu, Min, Xiong, Xiu-fang, Weng, Xiao-ling, Lu, Zhi-hui, Liang, Fei-li, Gong
Publikováno v:
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 21(5)
To construct the expression vector of biotin-protein ligase (BirA enzyme) gene and express the BirA enzyme with bioactivity in E.coli BL-21 (DE3).The BirA gene was amplified from E.coli genome by PCR and cloned into pGEX-4T-2 to construct the recombi
Publikováno v:
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi. 26(5)
To cultivate hematopoietic stem/progenitor cells (CD34(+)CD38(-)) isolated from umbilical cord blood (UCB) long for the observation of cell growth and expansion in vitro, surface marker expression, and chromosomal complements.By flow cytometry CD34-F
Publikováno v:
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 21(3)
To form soluble HLA-G1-peptide complex by refolding in vitro, and to study its immune function.The heavy chain and beta(2m) of sHLA-G1 were expressed as insoluble aggregates in E. coli, and then the two subunits were refolded to form HLA-G1-peptide c