Zobrazeno 1 - 10
of 62
pro vyhledávání: '"Fedor V. Subach"'
Autor:
Cuixin Lai, Lina Yang, Vishaka Pathiranage, Ruizhao Wang, Fedor V. Subach, Alice R. Walker, Kiryl D. Piatkevich
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-13 (2024)
Abstract Genetically encoded fluorescent biosensors became indispensable tools for biological research, enabling real-time observation of physiological processes in live cells. Recent protein engineering efforts have resulted in the generation of a l
Externí odkaz:
https://doaj.org/article/c2bd5b0fc912493db3eaef10e2dc2037
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
The detection of mercury ions is an important task in both environmental monitoring and cell biology research. However, existing genetically encoded sensors for mercury ions have certain limitations, such as negative fluorescence response, narrow dyn
Externí odkaz:
https://doaj.org/article/58b5a0d3e736417a94f882e6005e43a2
Publikováno v:
Heliyon, Vol 10, Iss 12, Pp e32814- (2024)
Heavy metals, particularly mercury, rank as some of the most hazardous systemic toxicants known to cause multiple organ damage, even at lower levels of exposure. Its detection in the environment and in the live cells is an actual task. Here, we engin
Externí odkaz:
https://doaj.org/article/467c92b09513441aa00de878c3d72fef
Autor:
Oksana M. Subach, Anna V. Vlaskina, Yulia K. Agapova, Alena Y. Nikolaeva, Anna M. Varizhuk, Oleg V. Podgorny, Kiryl D. Piatkevich, Maxim V. Patrushev, Konstantin M. Boyko, Fedor V. Subach
Publikováno v:
FEBS Open Bio, Vol 13, Iss 11, Pp 2047-2060 (2023)
Genetically encoded calcium indicators based on truncated troponin C are attractive probes for calcium imaging due to their relatively small molecular size and twofold reduced calcium ion buffering. However, the best‐suited members of this family,
Externí odkaz:
https://doaj.org/article/1f1660325fa647b5af79a6e6567d9c71
Autor:
Guo-Teng Liang, Cuixin Lai, Zejun Yue, Hanbin Zhang, Danyang Li, Zhong Chen, Xingyu Lu, Liang Tao, Fedor V. Subach, Kiryl D. Piatkevich
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Engineered light, oxygen, and voltage (LOV)-based proteins are able to fluoresce without oxygen requirement due to the autocatalytic incorporation of exogenous flavin as a chromophore thus allowing for live cell imaging under hypoxic and anaerobic co
Externí odkaz:
https://doaj.org/article/b28572dd584e40a4a7cb0d3a5ef945c5
Publikováno v:
Applied Sciences, Vol 9, Iss 15, p 3123 (2019)
We, the authors, wish to make the following corrections to our paper [...]
Externí odkaz:
https://doaj.org/article/9110a3372faa4bc48f242c549415bd26
Publikováno v:
Applied Sciences, Vol 9, Iss 3, p 562 (2019)
Our ability to investigate the brain is limited by available technologies that can record biological processes in vivo with suitable spatiotemporal resolution. Advances in optogenetics now enable optical recording and perturbation of central physiolo
Externí odkaz:
https://doaj.org/article/541c3df501b345438f598ac20eaee650
Autor:
Oksana M. Subach, Larisa Varfolomeeva, Anna V. Vlaskina, Yulia K. Agapova, Alena Y. Nikolaeva, Kiryl D. Piatkevich, Maxim V. Patrushev, Konstantin M. Boyko, Fedor V. Subach
Publikováno v:
Biochemical and Biophysical Research Communications. 665:169-177
Combined Structural and Computational Study of the mRubyFT Fluorescent Timer Locked in Its Blue Form
Autor:
Konstantin M. Boyko, Maria G. Khrenova, Alena Y. Nikolaeva, Pavel V. Dorovatovskii, Anna V. Vlaskina, Oksana M. Subach, Vladimir O. Popov, Fedor V. Subach
Publikováno v:
International Journal of Molecular Sciences; Volume 24; Issue 9; Pages: 7906
The mRubyFT is a monomeric genetically encoded fluorescent timer based on the mRuby2 fluorescent protein, which is characterized by the complete maturation of the blue form with the subsequent conversion to the red one. It has higher brightness in ma
Autor:
Siranush Babakhanova, Erica E. Jung, Kazuhiko Namikawa, Hanbin Zhang, Yangdong Wang, Oksana M. Subach, Dmitry A. Korzhenevskiy, Tatiana V. Rakitina, Xian Xiao, Wenjing Wang, Jing Shi, Mikhail Drobizhev, Demian Park, Lea Eisenhard, Hongyun Tang, Reinhard W. Köster, Fedor V. Subach, Edward S. Boyden, Kiryl D. Piatkevich
Publikováno v:
Protein Science. 31:728-751
In vivoimaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization of fluorescent proteins via directed molecular evolution in cultured mammalian