Výsledky vyhledávání - "Fawzi ALLALA"

Akademický článek

Biochemical characterization of an alkaline and detergent-stable Lipase from Fusarium annulatum Bugnicourt strain CBS associated with olive tree dieback.

Autor: Ahlem Dab, Ismail Hasnaoui, Sondes Mechri, Fawzi Allala, Khelifa Bouacem, Alexandre Noiriel, Amel Bouanane-Darenfed, Ennouamane Saalaoui, Abdeslam Asehraou, Fanghua Wang, Abdelkarim Abousalham, Bassem Jaouadi

Publikováno v: PLoS ONE, Vol 18, Iss 5, p e0286091 (2023)

This work describes a novel extracellular lipolytic carboxylester hydrolase named FAL, with lipase and phospholipase A1 (PLA1) activity, from a newly isolated filamentous fungus Ascomycota CBS strain, identified as Fusarium annulatum Bunigcourt. FAL

Externí odkaz: https://doaj.org/article/6f786c1b4bba4dacb7398b3e4d7cc644

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Akademický článek

Cloning, expression, and structural modeling of two alkaline serine protease genes from extremophilic Bacillaceae -related species: Application in valorization of invasive crustaceans

Autor: Sondes MECHRI, Khelifa BOUACEM, Fawzi ALLALA, Marwa KHALED, Amel BOUANANE-DARENFED, Hocine HACENE, Bassem JAOUADI

Publikováno v: Revue Nature et Technologie, Vol 13, Iss 02 (2022)

Two novel protease genes sapA and sapN from the thermophilic Anoxybacillus kamchatkensis M1V and Melghiribacillus thermohalophilus Nari2AT strains respectively, encoding a polypeptide of 381 and 379 residues, were identified, cloned and successfully

Externí odkaz: https://doaj.org/article/23e5001cbd5e4094845330a27f8eddc5

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Preparation, characterization, immobilization, and molecular docking analysis of a novel detergent-stable subtilisin-like serine protease from Streptomyces mutabilis strain TN-X30

Autor: Sondes Mechri, Fawzi Allala, Khelifa Bouacem, Ismail Hasnaoui, Hassan Gwaithan, Taha Bilel Chalbi, Ennouamane Saalaoui, Abdeslam Asehraou, Alexandre Noiriel, Abdelkarim Abousalham, Hocine Hacene, Amel Bouanane-Darenfed, Marilize Le Roes-Hill, Bassem Jaouadi

Publikováno v: International Journal of Biological Macromolecules. 222:1326-1342

We recently described the production of a detergent-biocompatible crude protease from Streptomyces mutabilis strain TN-X30. Here, we describe the purification, characterization, and immobilization of the serine alkaline protease (named SPSM), as well

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc8b824e7485127fcda18daf3787ee5c
https://doi.org/10.1016/j.ijbiomac.2022.09.161

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Cloning and heterologous expression of subtilisin SAPN, a serine alkaline protease from Melghiribacillus thermohalophilus Nari2AT in Escherichia coli and Pichia pastoris

Autor: Hocine Hacene, Laura Baciou, Nadia Zaraî Jaouadi, Céline Ferard, Sondes Mechri, Abdelkarim Abousalham, Khelifa Bouacem, Amel Bouanane-Darenfed, Florence Lederer, Fawzi Allala, Aicha Bouraoui, Hatem Rekik, Bassem Jaouadi, Alexandre Noiriel

Publikováno v: Process Biochemistry. 105:27-41

The sapN gene, encoding the extracellular subtilisin SAPN, a serine alkaline protease from Melghiribacillus thermohalophilus Nari2AT, was isolated, sequenced, and heterologously expressed in Escherichia coli BL21(DE3)pLysS using pUT57 and pTrc99A vec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aea18dade6937d6915df6590b5d327ff
https://doi.org/10.1016/j.procbio.2021.03.020

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Identification and homology modeling of a new biotechnologically compatible serine alkaline protease from moderately halotolerant Gracilibacillus boraciitolerans strain LO15

Autor: Akli Ouelhadj, Sondes Mechri, Merzouk Yahiaoui, Bassem Jaouadi, Khelifa Bouacem, Katia-Louiza Asmani, Fawzi Allala

Publikováno v: International Journal of Biological Macromolecules. 161:1456-1469

A new serine alkaline protease (designated as SAPGB) from Gracilibacillus boraciitolerans strain LO15, was produced (9000 U/mL), purified, and characterized. SAPGB has a monomer structure with a precise molecular weight of 30,285.03 kDa as learnt fro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc9a604c365b8fadd9c6841613088e45
https://doi.org/10.1016/j.ijbiomac.2020.07.266

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Characterization of chitinase from Shewanella inventionis HE3 with bio-insecticidal effect against granary weevil, Sitophilus granarius Linnaeus (Coleoptera: Curculionidae)

Autor: Merzouk Yahiaoui, Hassiba Laribi-Habchi, Bassem Jaouadi, Sondes Mechri, Fadoua Jabeur, Amel Bouanane-Darenfed, Okba Selama, Khelifa Bouacem, Fawzi Allala

Publikováno v: Process Biochemistry. 97:222-233

A 40 kDa chitinase from Shewanella inventionis HE3 was purified (ChiA-Si40) and characterized. Using fermentor with an optimized medium for 48 h at 37 °C, enzyme activity was enhanced by 10-times compared to those using shaking-flask-culture. Purifi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4be2f2e579736fe688a813a196aba7ff
https://doi.org/10.1016/j.procbio.2020.06.023

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Purification, biochemical, and molecular characterization of a novel extracellular thermostable and alkaline α-amylase from Tepidimonas fonticaldi strain HB23

Autor: Hocine Hacene, Mouna Sahnoun, Zahra Azzouz, Khelifa Bouacem, Fawzi Allala, Sondes Mechri, Amel Bouanane-Darenfed, Nawel Boucherba, Bassem Jaouadi, Said Benallaoua

Publikováno v: International Journal of Biological Macromolecules. 132:558-574

The present study investigated the purification, biochemical, and molecular characterization of a novel thermostable α-amylase (TfAmy48) from Tepidimonas fonticaldi strain HB23. MALDI-TOF/MS analysis indicated that the purified enzyme is a monomer w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95556a75f0e3d2d880f0fb640419157f
https://doi.org/10.1016/j.ijbiomac.2019.03.201

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