Cytokines and culture medium have a major impact on human in vitro T-cell differentiation

Autor: Julien Rouiller, Alexandrine Garrigue, Isabelle André-Schmutz, Estelle Morillon, Kheira Beldjord, Salima Hacein-Bey-Abina, Marina Cavazzana-Calvo, Delphine Bonhomme, Laure Cacavelli, Fatine Benjelloun, Johanna Blondeau, Emmanuelle Six

Publikováno v: Blood Cells, Molecules, and Diseases. 47:72-78

An important proof of principle has been achieved with the development of an in vitro T-cell differentiation assay based on the coculture of hematopoietic progenitors with the OP9-Delta1 stromal cell line. The original murine T cell differentiation a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ef176574b7eee77c35cc9c855105e50
https://doi.org/10.1016/j.bcmd.2011.04.001

Stable and functional lymphoid reconstitution in artemis-deficient mice following lentiviral artemis gene transfer into hematopoietic stem cells

Autor: Pierre Charneau, Julia Hauer, Julie Rivière, Marc Le Lorc'h, Jean-Pierre de Villartay, Pauline Soulas-Sprauel, Anne Galy, Michèle Malassis-Séris, Johanna Blondeau, Alexandrine Garrigue, Alain Fischer, Frederique Pâques, Nicole Brousse, Marina Cavazzana-Calvo, Serge Romana, Fatine Benjelloun, Daniel Stockholm, Annick Lim, Corinne Demerens-de Chappedelaine

Publikováno v: Molecular Therapy
Molecular Therapy, Cell Press, 2008, 16 (8), pp.1490-9. ⟨10.1038/mt.2008.118⟩
Molecular Therapy, 2008, 16 (8), pp.1490-1499. ⟨10.1038/mt.2008.118⟩
Molecular Therapy, Nature Publishing Group, 2008, 16 (8), pp.1490-9. 〈10.1038/mt.2008.118〉
Molecular Therapy, 2008, 16 (8), pp.1490-9. ⟨10.1038/mt.2008.118⟩
Molecular Therapy, Nature Publishing Group, 2008, 16 (8), pp.1490-9. ⟨10.1038/mt.2008.118⟩

International audience; Patients with mutations in the Artemis gene display a complete absence of T- and B lymphocytes, together with increased cellular radiosensitivity; this leads to a radiosensitive severe combined immunodeficiency (RS-SCID). Allo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd124b09ba43175b099dad8fc5b27673
https://pubmed.ncbi.nlm.nih.gov/18560421

Structural involvement in substrate recognition of an essential aspartate residue conserved in Mep/Amt and Rh-type ammonium transporters

Autor: Baya Cherif-Zahar, Fatine Benjelloun, Anna Maria Marini, Mélanie Boeckstaens, Bruno André

Publikováno v: Current genetics. 49(6)

Ammonium transport proteins belonging to the Mep/Amt/Rh family are spread throughout all domains of life. A conserved aspartate residue plays a key role in the function of Escherichia coli AmtB. Here, we show that the analogous aspartate residue is c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddb5727cfafedffd6f79d4e1c6f2074c
https://pubmed.ncbi.nlm.nih.gov/16477434

DNA bar coding and pyrosequencing to analyze adverse events in therapeutic gene transfer

Autor: Salima Hacein-Bey-Abina, Fatine Benjelloun, Gary P. Wang, Keshet Ronen, Jeremy Leipzig, Marina Cavazzana-Calvo, Chantal Lagresle-Peyrou, Alain Fischer, Charles C. Berry, Frederic D. Bushman, Angela Ciuffi, Alexandrine Garrigue

Publikováno v: Nucleic Acids Research

Gene transfer has been used to correct inherited immunodeficiencies, but in several patients integration of therapeutic retroviral vectors activated proto-oncogenes and caused leukemia. Here, we describe improved methods for characterizing integratio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f813bcefd98ee98871b57a7fcbc924d8
http://europepmc.org/articles/PMC2396413