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pro vyhledávání: '"Fatima D'Souza"'
Autor:
Fatima D'Souza, Arvind M. Lali
Publikováno v:
Biotechnology Techniques. 13:59-63
Chelating carboxymethyl cellulose was prepared in bead form by immobilizing iminodiacetic acid on carboxymethyl cellulose which was earlier crosslinked and activated by epichlorohydrin. The prepared matrix was used to purify papain by a factor of 2.6
Publikováno v:
Bioseparation. 7:195-205
Affinity precipitation is a technique that imparts selectivity to the widely used primary purification step of precipitation of proteins from crude extracts. Hetero-bifunctional affinity precipitation involves use of reversibly soluble/insoluble poly