Bacterial RNA sensing by TLR8 requires RNase 6 processing and is inhibited by RNA 2'O-methylation.

Autor: Nunes IV; Dept. of Infectious Diseases, Medical Microbiology and Hygiene, Medical Faculty, Heidelberg University, Heidelberg, Germany.; University Hospital Heidelberg, Heidelberg, Germany., Breitenbach L; Dept. of Infectious Diseases, Medical Microbiology and Hygiene, Medical Faculty, Heidelberg University, Heidelberg, Germany.; University Hospital Heidelberg, Heidelberg, Germany., Pawusch S; Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institute, Greifswald, Germany., Eigenbrod T; Dept. of Infectious Diseases, Medical Microbiology and Hygiene, Medical Faculty, Heidelberg University, Heidelberg, Germany.; Institute of Laboratory Medicine, SLK Clinics Heilbronn GmbH, 74078, Heilbronn, Germany., Ananth S; Department of Infectious Diseases, Center for Integrative Infectious Disease Research (CIID), Integrative Virology, Heidelberg University, Medical Faculty Heidelberg, Heidelberg, Germany., Schad P; Dept. of Infectious Diseases, Medical Microbiology and Hygiene, Medical Faculty, Heidelberg University, Heidelberg, Germany., Fackler OT; Department of Infectious Diseases, Center for Integrative Infectious Disease Research (CIID), Integrative Virology, Heidelberg University, Medical Faculty Heidelberg, Heidelberg, Germany.; German Center for Infection Research (DZIF), Heidelberg Partner Site, Heidelberg, Germany., Butter F; Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institute, Greifswald, Germany., Dalpke AH; Dept. of Infectious Diseases, Medical Microbiology and Hygiene, Medical Faculty, Heidelberg University, Heidelberg, Germany. alexander.dalpke@med.uni-heidelberg.de.; University Hospital Heidelberg, Heidelberg, Germany. alexander.dalpke@med.uni-heidelberg.de., Chen LS; Dept. of Infectious Diseases, Medical Microbiology and Hygiene, Medical Faculty, Heidelberg University, Heidelberg, Germany.; University Hospital Heidelberg, Heidelberg, Germany.

Publikováno v: EMBO reports [EMBO Rep] 2024 Nov; Vol. 25 (11), pp. 4674-4692. Date of Electronic Publication: 2024 Oct 03.

ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting.

Autor: Tashev SA; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.; School of Chemistry, College of Engineering and Physical Sciences, University of Birmingham, Birmingham, UK.; Centre of Membrane Proteins and Receptors, The Universities of Birmingham and Nottingham, Birmingham, UK., Euchner J; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.; School of Chemistry, College of Engineering and Physical Sciences, University of Birmingham, Birmingham, UK.; Centre of Membrane Proteins and Receptors, The Universities of Birmingham and Nottingham, Birmingham, UK.; Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany., Yserentant K; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.; School of Chemistry, College of Engineering and Physical Sciences, University of Birmingham, Birmingham, UK.; Centre of Membrane Proteins and Receptors, The Universities of Birmingham and Nottingham, Birmingham, UK.; Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany.; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA., Hänselmann S; Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany., Hild F; Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany., Chmielewicz W; Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany.; Department of Infectious Diseases, Integrative Virology, University Hospital Heidelberg, Heidelberg, Germany., Hummert J; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.; School of Chemistry, College of Engineering and Physical Sciences, University of Birmingham, Birmingham, UK.; Centre of Membrane Proteins and Receptors, The Universities of Birmingham and Nottingham, Birmingham, UK.; Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany.; PicoQuant GmbH, Berlin, Germany., Schwörer F; Division Systems Biology of Signal Transduction, German Cancer Research Center (DKFZ), Heidelberg, Germany., Tsopoulidis N; Department of Infectious Diseases, Integrative Virology, University Hospital Heidelberg, Heidelberg, Germany., Germer S; Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany., Saßmannshausen Z; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.; Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany., Fackler OT; Department of Infectious Diseases, Integrative Virology, University Hospital Heidelberg, Heidelberg, Germany., Klingmüller U; Division Systems Biology of Signal Transduction, German Cancer Research Center (DKFZ), Heidelberg, Germany., Herten DP; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK. d.herten@bham.ac.uk.; School of Chemistry, College of Engineering and Physical Sciences, University of Birmingham, Birmingham, UK. d.herten@bham.ac.uk.; Centre of Membrane Proteins and Receptors, The Universities of Birmingham and Nottingham, Birmingham, UK. d.herten@bham.ac.uk.; Institute of Physical Chemistry, Heidelberg University, Heidelberg, Germany. d.herten@bham.ac.uk.

Publikováno v: Nature methods [Nat Methods] 2024 Sep; Vol. 21 (9), pp. 1708-1715. Date of Electronic Publication: 2024 Aug 08.

Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells.

Autor: Albanese M; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany; Department for Clinical Sciences and Community Health (DISCCO), University of Milan, Milan, Italy., Chen HR; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany. Electronic address: hong-ru@besson-girard.fr., Gapp M; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Muenchhoff M; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany; German Centre for Infection Research (DZIF), Partner Site Munich, Munich, Germany., Yang HH; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Peterhoff D; Institute of Medical Microbiology and Hygiene, Molecular Microbiology (Virology), University of Regensburg, Regensburg, Germany., Hoffmann K; Institute of Virology, University Medical Center, Albert-Ludwigs-University Freiburg, Freiburg, Germany., Xiao Q; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Ruhle A; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Ambiel I; Department of Infectious Diseases, Heidelberg University, Medical Faculty Heidelberg, Integrative Virology, Center for Integrative Infectious Disease Research (CIID), Heidelberg, Germany; German Centre for Infection Research (DZIF), Partner Site Heidelberg, Heidelberg, Germany., Schneider S; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Mejías-Pérez E; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Stern M; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Wratil PR; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Hofmann K; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Amann L; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Jocham L; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Fuchs T; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany., Ulivi AF; Max Planck Institute of Psychiatry, Munich, Germany., Besson-Girard S; Institute for Stroke and Dementia Research, University Hospital, LMU München, Munich, Germany., Weidlich S; Technical University of Munich, School of Medicine, University Hospital Rechts der Isar, Department of Internal Medicine II, Munich, Germany., Schneider J; Technical University of Munich, School of Medicine, University Hospital Rechts der Isar, Department of Internal Medicine II, Munich, Germany., Spinner CD; German Centre for Infection Research (DZIF), Partner Site Munich, Munich, Germany; Technical University of Munich, School of Medicine, University Hospital Rechts der Isar, Department of Internal Medicine II, Munich, Germany., Sutter K; University Hospital Essen, University Duisburg-Essen, Institute for Virology and Institute for Translational HIV Research, Essen, Germany., Dittmer U; University Hospital Essen, University Duisburg-Essen, Institute for Virology and Institute for Translational HIV Research, Essen, Germany., Humpe A; Department of Transfusion Medicine, Cell Therapeutics, and Hemostaseology, Department of Anesthesiology, University Hospital Munich, Munich, Germany., Baumeister P; Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital, LMU München, Munich, Germany., Wieser A; German Centre for Infection Research (DZIF), Partner Site Munich, Munich, Germany; Max von Pettenkofer Institute, Medical Microbiology and Hospital Epidemiology, Faculty of Medicine, LMU München, Munich, Germany; Division of Infectious Diseases and Tropical Medicine, University Hospital, LMU München, Munich, Germany., Rothenfusser S; Division of Clinical Pharmacology, University Hospital, LMU München and Unit Clinical Pharmacology (EKliP), Helmholtz Center for Environmental Health, Munich, Germany., Bogner J; German Centre for Infection Research (DZIF), Partner Site Munich, Munich, Germany; Division of Infectious Diseases, University Hospital, Medizinische Klinik und Poliklinik IV, LMU München, Munich, Germany., Roider J; German Centre for Infection Research (DZIF), Partner Site Munich, Munich, Germany; Division of Infectious Diseases, University Hospital, Medizinische Klinik und Poliklinik IV, LMU München, Munich, Germany., Knolle P; German Centre for Infection Research (DZIF), Partner Site Munich, Munich, Germany; Institute of Molecular Immunology and Experimental Oncology, School of Medicine, Technical University of Munich (TUM), Munich, Germany., Hengel H; Institute of Virology, University Medical Center, Albert-Ludwigs-University Freiburg, Freiburg, Germany., Wagner R; Institute of Medical Microbiology and Hygiene, Molecular Microbiology (Virology), University of Regensburg, Regensburg, Germany., Laketa V; German Centre for Infection Research (DZIF), Partner Site Heidelberg, Heidelberg, Germany; Department of Infectious Diseases, Heidelberg University, Medical Faculty Heidelberg, Virology, Center for Integrative Infectious Disease Research (CIID), Heidelberg, Germany., Fackler OT; Department of Infectious Diseases, Heidelberg University, Medical Faculty Heidelberg, Integrative Virology, Center for Integrative Infectious Disease Research (CIID), Heidelberg, Germany; German Centre for Infection Research (DZIF), Partner Site Heidelberg, Heidelberg, Germany. Electronic address: oliver.fackler@med.uni-heidelberg.de., Keppler OT; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany; German Centre for Infection Research (DZIF), Partner Site Munich, Munich, Germany. Electronic address: keppler@mvp.lmu.de.

Publikováno v: Cell reports. Medicine [Cell Rep Med] 2024 Apr 16; Vol. 5 (4), pp. 101483. Date of Electronic Publication: 2024 Apr 04.

Eliciting CD4-mimicking broadly neutralizing antibodies: new avenues towards the rational design of an HIV vaccine.

Autor: Muecksch F; Department of Infectious Diseases, Heidelberg University, Medical Faculty Heidelberg, Virology, Center for Integrative Infectious Disease Research (CIID), Heidelberg, Germany.; Chica and Heinz Schaller (CHS) Research Group, Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany., Fackler OT; Department of Infectious Diseases, Heidelberg University, Medical Faculty Heidelberg, Integrative Virology, Center for Integrative Infectious Disease Research (CIID), Heidelberg, Germany. oliver.fackler@med.uni-heidelberg.de.; German Centre for Infection Research (DZIF), Partner Site Heidelberg, Heidelberg, Germany. oliver.fackler@med.uni-heidelberg.de.

Publikováno v: Signal transduction and targeted therapy [Signal Transduct Target Ther] 2024 Feb 29; Vol. 9 (1), pp. 49. Date of Electronic Publication: 2024 Feb 29.

Activation-neutral gene editing of tonsillar CD4 T cells for functional studies in human ex vivo tonsil cultures.

Autor: Morath K; Department of Infectious Diseases, Integrative Virology, University Hospital Heidelberg, Im Neuenheimer Feld 344, 69120 Heidelberg, Germany., Sadhu L; Department of Infectious Diseases, Integrative Virology, University Hospital Heidelberg, Im Neuenheimer Feld 344, 69120 Heidelberg, Germany., Dyckhoff G; Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany., Gapp M; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig-Maximilians-Universität München, Pettenkoferstraße 9a, 80336 Munich, Germany., Keppler OT; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig-Maximilians-Universität München, Pettenkoferstraße 9a, 80336 Munich, Germany; German Centre for Infection Research (DZIF), Partner Site München, Munich, Germany., Fackler OT; Department of Infectious Diseases, Integrative Virology, University Hospital Heidelberg, Im Neuenheimer Feld 344, 69120 Heidelberg, Germany; German Centre for Infection Research (DZIF), Partner Site Heidelberg, Heidelberg, Germany. Electronic address: oliver.fackler@med.uni-heidelberg.de.

Publikováno v: Cell reports methods [Cell Rep Methods] 2024 Jan 22; Vol. 4 (1), pp. 100685. Date of Electronic Publication: 2024 Jan 10.