Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies

Autor: Laurent Blanchoin, Manuel Théry, Julia Mahamid, Fabrice Senger, Mauricio Toro-Nahuelpan, Ievgeniia Zagoriy

Publikováno v: Nature Methods
Nature Methods, Nature Publishing Group, In press, ⟨10.1038/s41592-019-0630-5⟩
bioRxiv
Nature Methods, Nature Publishing Group, In press, 17 (1), pp.50-54. ⟨10.1038/s41592-019-0630-5⟩
Nature Methods, In press, 17 (1), pp.50-54. ⟨10.1038/s41592-019-0630-5⟩
Nature methods

Micromachining by cryo-focused ion beam (FIB) milling coupled to cryo-electron tomography (ET) enables visualization of macromolecules directly inside cells. Yet, spatial control of cell adhesion on electron microscopy (EM) grids remains a bottleneck

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cdb2d100a98ee90733d7cd0d2277bfd
https://hal.archives-ouvertes.fr/hal-02379109

Spatial integration of mechanical forces by alpha-actinin establishes actin network symmetry

Autor: Manuel Théry, Hajer Ennomani, Laurent Blanchoin, Fabrice Senger, Laetitia Kurzawa, Amandine Pitaval

Cell and tissue morphogenesis depend on the production and spatial organization of tensional forces in the actin cytoskeleton. Actin network architecture is complex because it is made of distinct modules in which filaments adopt a variety of organiza

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbdebfb419411e9e594a0b183f494bc4

Microtubule stabilization drives 3D centrosome migration to initiate primary ciliogenesis

Autor: Gaëlle Letort, Fabrice Senger, Xavier Gidrol, Laurent Guyon, Manuel Théry, Amandine Pitaval, James Sillibourne

Publikováno v: Journal of Cell Biology
Journal of Cell Biology, Rockefeller University Press, 2017, 216 (11), pp.3713-3728. ⟨10.1083/jcb.201610039⟩
The Journal of Cell Biology
Journal of Cell Biology, 2017, 216 (11), pp.3713-3728. ⟨10.1083/jcb.201610039⟩

The classical view of centrosome decentering and migration to the cell periphery during ciliogenesis is that it is pulled toward its final destination. Here, Pitaval et al. argue that microtubule stabilization in the early stages of ciliogenesis gene

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::794a8fadc54f2c61b4e7b2038544bd85
https://hal.archives-ouvertes.fr/hal-01666743

Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation

Autor: Hui-yuan Tseng, Reinhard Fässler, Naoko Mizuno, Alexander R. Dunn, Dirk Dedden, Fabrice Senger, Laetitia Kurzawa, Manuel Théry, Steven Tan, Zhiqi Sun, Anita A. Wasik

Publikováno v: Nature Cell Biology
Nature Cell Biology, Nature Publishing Group, 2016, 18 (9), pp.941-953
Nature Cell Biology, Nature Publishing Group, 2016, 18 (9), pp.941-953. ⟨10.1038/ncb3402⟩
Nature Cell Biology, 2016, 18 (9), pp.941-953. ⟨10.1038/ncb3402⟩

International audience; Integrin-based adhesions play critical roles in cell migration. Talin activates integrins and flexibly connects integrins to the actomyosin cytoskeleton, thereby serving as a 'molecular clutch' that transmits forces to the ext

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77873e1d4d255a6397ab7e8e992863b5
https://hal.archives-ouvertes.fr/hal-01414459

Fast high-resolution 3D total internal reflection fluorescence microscopy by incidence angle scanning and azimuthal averaging

Autor: Bertrand Cinquin, Jérôme Boulanger, Perrine Paul-Gilloteaux, Jean Salamero, Laurent Blanchoin, Sabine Bardin, Fabrice Senger, Charles Gueudry, Daniel Münch, Christophe Guérin

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2014, 111 (48), pp.17164-9
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2014, 111, pp.17164-17169. ⟨10.1073/pnas.1414106111⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2014, 111 (48), pp.17164-9

Significance Recent progress has pushed forward the resolving capacity of optical microscopy at the expense of a low acquisition rate and use of specific probes. Such limitations make these techniques incompatible with dynamics localization of multip

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aab4ec2c8569c1393af790b1ad51232d
https://hal.science/hal-01133826