Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Fabien Piguet"'
Autor:
Fabien Piguet, Hadjer Ouldali, Manuela Pastoriza-Gallego, Philippe Manivet, Juan Pelta, Abdelghani Oukhaled
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
Existing methods used for peptide analysis suffer from low sensitivity and specificity. Here the authors demonstrate the nanopore-based size-discrimination and purity analysis of short homopolymeric peptides with a single amino acid resolution.
Externí odkaz:
https://doaj.org/article/1633ddd585644efcac9f78479969cdde
Autor:
Mazdak Afshar Bakshloo, Safia Yahiaoui, Fabien Piguet, Manuela Pastoriza-Gallego, Régis Daniel, Jérôme Mathé, John J. Kasianowicz, Abdelghani Oukhaled
Publikováno v:
Nano Research
Nano Research, 2022, 15 (11), pp.9831-9842. ⟨10.1007/s12274-022-4610-1⟩
Nano Research, 2022, 15 (11), pp.9831-9842. ⟨10.1007/s12274-022-4610-1⟩
International audience
Autor:
Mazdak Afshar Bakshloo, John J. Kasianowicz, Manuela Pastoriza-Gallego, Jérôme Mathé, Régis Daniel, Fabien Piguet, Abdelghani Oukhaled
Publikováno v:
Journal of the American Chemical Society
Journal of the American Chemical Society, 2022, 144 (6), pp.2716-2725. ⟨10.1021/jacs.1c11758⟩
Journal of the American Chemical Society, 2022, 144 (6), pp.2716-2725. ⟨10.1021/jacs.1c11758⟩
The implementation of a reliable, rapid, inexpensive, and simple method for whole-proteome identification would greatly benefit cell biology research and clinical medicine. Proteins are currently identified by cleaving them with proteases, detecting
Autor:
Mazdak Afshar Bakshloo, Safia Yahiaoui, Hadjer Ouldali, Manuela Pastoriza‐Gallego, Fabien Piguet, Abdelghani Oukhaled
Publikováno v:
Proteomics
Proteomics, 2022, pp.2100056. ⟨10.1002/pmic.202100056⟩
Proteomics, 2022, pp.2100056. ⟨10.1002/pmic.202100056⟩
Nanopore-based single-molecule analysis technique is a promising approach in the field of proteomics. In this Technical Brief, the interaction between the biological nanopore of Aerolysin (AeL) and peptides is investigated, focusing on potential bias
Autor:
Abdelghani Oukhaled, Hadjer Ouldali, Juan Pelta, Kumar Sarthak, Tobias Ensslen, Philippe Manivet, Aleksei Aksimentiev, Jan C. Behrends, Fabien Piguet
Publikováno v:
Nature Biotechnology. 38:176-181
Efforts to sequence single protein molecules in nanopores1-5 have been hampered by the lack of techniques with sufficient sensitivity to discern the subtle molecular differences among all twenty amino acids. Here we report ionic current detection of
Autor:
Fabien, Piguet, Tobias, Ensslen, Mazdak A, Bakshloo, Monasadat, Talarimoghari, Hadjer, Ouldali, Gerhard, Baaken, Ekaterina, Zaitseva, Manuela, Pastoriza-Gallego, Jan C, Behrends, Abdelghani, Oukhaled
Publikováno v:
Methods in enzymology. 649
We report here on the nanopore resistive pulse sensing (Np-RPS) method, involving pore-forming toxins as tools for polymer analytics at single molecule level. Np-RPS is an electrical method for the label-free detection of single molecules. A molecule
Autor:
Gerhard Baaken, Ekaterina Zaitseva, Manuela Pastoriza-Gallego, Jan C. Behrends, Hadjer Ouldali, Tobias Ensslen, Mazdak Afshar Bakshloo, Fabien Piguet, Monasadat Talarimoghari, Abdelghani Oukhaled
Publikováno v:
Methods in Enzymology ISBN: 9780128238585
Pore-Forming Toxins
Pore-Forming Toxins, 649, Elsevier, pp.587-634, 2021, Methods in Enzymology, ⟨10.1016/bs.mie.2021.01.017⟩
Pore-Forming Toxins
Pore-Forming Toxins, 649, Elsevier, pp.587-634, 2021, Methods in Enzymology, ⟨10.1016/bs.mie.2021.01.017⟩
We report here on the nanopore resistive pulse sensing (Np-RPS) method, involving pore-forming toxins as tools for polymer analytics at single molecule level. Np-RPS is an electrical method for the label-free detection of single molecules. A molecule
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7d28d4145e6ee2ebff1e46f95fa96a6
https://doi.org/10.1016/bs.mie.2021.01.017
https://doi.org/10.1016/bs.mie.2021.01.017
Autor:
Manuela Pastoriza-Gallego, Juan Pelta, Hadjer Ouldali, Abdelghani Oukhaled, Philippe Manivet, Fabien Piguet
Publikováno v:
Nature Communications
Nature Communications, 2018, 9 (1), pp.966. ⟨10.1038/S41467-018-03418-2⟩
Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.966. ⟨10.1038/S41467-018-03418-2⟩
Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
Nature Communications, 2018, 9 (1), pp.966. ⟨10.1038/S41467-018-03418-2⟩
Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.966. ⟨10.1038/S41467-018-03418-2⟩
Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
There are still unmet needs in finding new technologies for biomedical diagnostic and industrial applications. A technology allowing the analysis of size and sequence of short peptide molecules of only few molecular copies is still challenging. The f
Autor:
Juan Pelta, Abdelghani Oukhaled, Francoise Discala, Marie-France Breton, Jan C. Behrends, Hadjer Ouldali, Fabien Piguet
Publikováno v:
Scientific Reports
Scientific Reports, 2016, 6 (1), pp.38675. ⟨10.1038/srep38675⟩
Scientific Reports, Nature Publishing Group, 2016, 6 (1), pp.38675. ⟨10.1038/srep38675⟩
Scientific Reports, 2016, 6 (1), pp.38675. ⟨10.1038/srep38675⟩
Scientific Reports, Nature Publishing Group, 2016, 6 (1), pp.38675. ⟨10.1038/srep38675⟩
We explore the effect of temperature on the interaction of polydisperse mixtures of nonionic poly(ethylene glycol) (PEG) polymers of different average molar masses with the biological nanopore α-hemolysin. In contrast with what has been previously o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e699ad6e3cf8c35ad30907de7aae9f6b
https://hal.science/hal-02065255/file/srep38675.pdf
https://hal.science/hal-02065255/file/srep38675.pdf
Autor:
Abdelghani Oukhaled, Laurent Bacri, Fabien Piguet, Francoise Discala, Juan Pelta, Marie-France Breton
Publikováno v:
Journal of Physical Chemistry Letters
Journal of Physical Chemistry Letters, 2014, 5 (24), pp.4362-4367. ⟨10.1021/jz502360c⟩
Journal of Physical Chemistry Letters, American Chemical Society, 2014, 5 (24), pp.4362-4367. ⟨10.1021/jz502360c⟩
Journal of Physical Chemistry Letters, 2014, 5 (24), pp.4362-4367. ⟨10.1021/jz502360c⟩
Journal of Physical Chemistry Letters, American Chemical Society, 2014, 5 (24), pp.4362-4367. ⟨10.1021/jz502360c⟩
International audience; We demonstrate experimentally the existence of an electroosmotic flow (EOF) through the wild-type nanopore of α-hemolysin in a large range of applied voltages and salt concentrations for two different salts, LiCl and KCl. EOF
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::511feebc3274039f200d3328a9e2dec7
https://hal.science/hal-02006492
https://hal.science/hal-02006492