Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Fabian U. Zwettler"'
Autor:
Fabian U. Zwettler, Sebastian Reinhard, Davide Gambarotto, Toby D. M. Bell, Virginie Hamel, Paul Guichard, Markus Sauer
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Previous attempts to combine expansion microscopy (ExM) and single molecule localisation microscopy (SMLM) have proved challenging. Here the authors show that post-labelling Ex-SMLM improves labelling efficiency, reduces linkage error, and preserves
Externí odkaz:
https://doaj.org/article/138518bbdfb845248b9023cf52af2865
Autor:
Fabian U. Zwettler, Marie-Christin Spindler, Sebastian Reinhard, Teresa Klein, Andreas Kurz, Ricardo Benavente, Markus Sauer
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
The authors test expansion microscopy protocols for investigation of the molecular organisation of mammalian synaptonemal complexes (SCs) with structured illumination microscopy. They show that the molecular structure is preserved during expansion an
Externí odkaz:
https://doaj.org/article/11cb7bca60d54e4b80ed2a6bc218b85c
Publikováno v:
Methods in cell biology. 161
This chapter provides a step-by-step protocol how to prepare expansion microcoscopy (ExM) treated biological samples for imaging with single-molecule localization microscopy (SMLM). For this purpose, the protocol describes the stabilization of expand
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a904de2a34cb4b6ce76f1a0a5d3fd4d4
https://pubmed.ncbi.nlm.nih.gov/33478695
https://pubmed.ncbi.nlm.nih.gov/33478695
Publikováno v:
Methods in Cell Biology ISBN: 9780128208076
This chapter provides a step-by-step protocol how to prepare expansion microcoscopy (ExM) treated biological samples for imaging with single-molecule localization microscopy (SMLM). For this purpose, the protocol describes the stabilization of expand
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c0cd6ed0dbc8fda095464d8d35366aa6
https://doi.org/10.1016/bs.mcb.2020.05.004
https://doi.org/10.1016/bs.mcb.2020.05.004
Autor:
Paul Guichard, Virginie Hamel, Sebastian Reinhard, Fabian U. Zwettler, Toby D. M. Bell, Davide Gambarotto, Markus Sauer
Publikováno v:
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Expansion microscopy (ExM) enables super-resolution fluorescence imaging of physically expanded biological samples with conventional microscopes. By combining ExM with single-molecule localization microscopy (SMLM) it is potentially possible to appro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da80c9764cb2df15446d36dfe3a9844d
https://doi.org/10.1038/s41467-020-17086-8
https://doi.org/10.1038/s41467-020-17086-8
Autor:
Marie-Christin Spindler, Sebastian Reinhard, Markus Sauer, Fabian U. Zwettler, Teresa Klein, Andreas Kurz, Ricardo Benavente
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications
Nature Communications
The synaptonemal complex (SC) is a meiosis-specific nuclear multiprotein complex that is essential for proper synapsis, recombination and segregation of homologous chromosomes. We combined structured illumination microscopy (SIM) with different expan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::576ff5ffd17d8ba949128075ee63cabc
Autor:
Gregory W. Moseley, Donna R. Whelan, Sam Duwé, Ashley M. Rozario, Fabian U. Zwettler, Aaron Brice, Peter Dedecker, Toby D. M. Bell, Riley B. Hargreaves, Markus Sauer
Publikováno v:
Australian Journal of Chemistry. 73:686
The field of super-resolution microscopy continues to progress rapidly, both in terms of evolving techniques and methodologies as well as in the development of new multi-disciplinary applications. Two current drivers of innovation are increasing the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1154e1b170ee34c5fe37b3d15fafb572
https://doi.org/10.1071/ch19571
https://doi.org/10.1071/ch19571
Autor:
Marketa Cernohorska, Paul Guichard, Jan-Gero Schloetel, Susanne Borgers, Edward S. Boyden, Fabian U. Zwettler, Davide Gambarotto, Markus Sauer, Michael Unser, Jörn Heine, Matthias Reuss, Virginie Hamel, Denis Fortun
For decades, electron microscopy (EM) was the only method able to reveal the ultrastructure of cellular organelles and molecular complexes because of the diffraction limit of optical microscopy. In recent past, the emergence of superresolution fluore
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01c54a811334d259b9735cac327eb955
https://doi.org/10.1101/308270
https://doi.org/10.1101/308270
Publikováno v:
Nature communications. 4
Web spiders assemble spidroin monomers into silk fibres of unrivalled tensile strength at remarkably high spinning speeds of up to 1 m s(-1). The spidroin N-terminal domain contains a charge-driven, pH-sensitive relay that controls self-association b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf99ffd8f837e66be9527e9b1e82b63b
https://pubmed.ncbi.nlm.nih.gov/24240554
https://pubmed.ncbi.nlm.nih.gov/24240554
Autor:
Jan-Gero Schloetel, Virginie Hamel, Marketa Schmidt-Cernohorska, Matthias Reuss, Michael Unser, Susanne Borgers, Maeva Le Guennec, Joern Heine, Edward S. Boyden, Fabian U. Zwettler, Davide Gambarotto, Denis Fortun, Paul Guichard, Markus Sauer
Publikováno v:
Nature Methods
Nature Methods, 2018
Nature methods
Nature Methods, Nature Publishing Group, 2018
Nature Methods, 2018
Nature methods
Nature Methods, Nature Publishing Group, 2018
Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6af07748da698812eb9015609fb786e
https://infoscience.epfl.ch/record/263446
https://infoscience.epfl.ch/record/263446