Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Fabian B. H. Rehm"'
Autor:
Junqiao Du, Kuok Yap, Lai Yue Chan, Fabian B. H. Rehm, Fong Yang Looi, Aaron G. Poth, Edward K. Gilding, Quentin Kaas, Thomas Durek, David J. Craik
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Asparaginyl endopeptidases (AEPs) catalyze the cyclization step during the biosynthesis of cyclic peptides in plants. Here, the authors report a recombinantly produced AEP that catalyzes the backbone cyclization of a linear cyclotide precursor and an
Externí odkaz:
https://doaj.org/article/2374d960c8e2417095bdec822494ebdd
Publikováno v:
Molecules, Vol 21, Iss 10, p 1370 (2016)
Enzymes are used as biocatalysts in a vast range of industrial applications. Immobilization of enzymes to solid supports or their self-assembly into insoluble particles enhances their applicability by strongly improving properties such as stability i
Externí odkaz:
https://doaj.org/article/eb7c9cbbc3204ec6adac680adc46b3cf
Autor:
Fabian B. H. Rehm, Simon J. de Veer, Linda H.L. Lua, Shyn Ric Tang, Yan Zhou, Conan K. Wang, Thomas Durek, David J. Craik, Junqiao Du, Kuok Yap, Jing Xie
Publikováno v:
Nature Protocols. 16:1740-1760
Cyclic disulfide-rich peptides have attracted significant interest in drug development and biotechnology. Here, we describe a protocol for producing cyclic peptide precursors in Pichia pastoris that undergo in vitro enzymatic maturation into cyclic p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::78359db5bb7f40ba35d5152773d88949
https://doi.org/10.1038/s41596-020-00483-0
https://doi.org/10.1038/s41596-020-00483-0
Publikováno v:
Angewandte Chemie. 133:4050-4054
The use of enzymes for the site-specific modification of proteins/peptides has become a highly accessible, widespread approach to study protein/peptide functions or to generate therapeutic conjugates. Asparaginyl endopeptidases (AEPs) that preferenti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a680ba6a243de1e150678c82d0710f8
https://doi.org/10.1002/ange.202013584
https://doi.org/10.1002/ange.202013584
Autor:
Quentin Kaas, Edward K. Gilding, David J. Craik, Fong Yang Looi, Lai Yue Chan, Thomas Durek, Kuok Yap, Junqiao Du, Aaron G. Poth, Fabian B. H. Rehm
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications
Nature Communications
Asparaginyl endopeptidases (AEPs) catalyze the key backbone cyclization step during the biosynthesis of plant-derived cyclic peptides. Here, we report the identification of two AEPs from Momordica cochinchinensis and biochemically characterize MCoAEP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e744473c7211021896367957acb68339
https://doaj.org/article/2374d960c8e2417095bdec822494ebdd
https://doaj.org/article/2374d960c8e2417095bdec822494ebdd
Autor:
David J. Craik, Fabian B. H. Rehm, Anuja R. Bony, Kuok Yap, Shyn Ric Tang, Simon J. de Veer, Junqiao Du, Fong Yang Looi, Conan K. Wang, David J. Adams, Jing Xie, Linda H.L. Lua, Lai Yue Chan, Thomas Durek
Publikováno v:
Green Chemistry. 22:5002-5016
Macrocyclic, disulfide-rich peptides have found widespread applications in drug design and development. Current peptide production strategies rely heavily on solid phase peptide synthesis (SPPS) requiring large amounts of hazardous/toxic reagents and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01a7383e9423cf392e3c3b6663a8b56b
https://doi.org/10.1039/d0gc01366h
https://doi.org/10.1039/d0gc01366h
Autor:
Choi Yi Li, Fabian B. H. Rehm, Kuok Yap, Christina N. Zdenek, Maxim D. Harding, Bryan G. Fry, Thomas Durek, David J. Craik, Simon J. de Veer
Publikováno v:
Angewandte Chemie (International ed. in English). 61(19)
Knottins are topologically complex peptides that are stabilised by a cystine knot and have exceptionally diverse functions, including protease inhibition. However, approaches for tuning their activity in situ are limited. Here, we demonstrate separat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a487a2ad692a4b8f125ee580aecc44f
https://pubmed.ncbi.nlm.nih.gov/35224831
https://pubmed.ncbi.nlm.nih.gov/35224831
Publikováno v:
Angewandte Chemie (International ed. in English). 61(11)
Transpeptidase-catalyzed protein and peptide modifications have been widely utilized for generating conjugates of interest for biological investigation or therapeutic applications. However, all known transpeptidases are constrained to ligating in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fc558253885e1fcb380121e99490d08
https://pubmed.ncbi.nlm.nih.gov/35018698
https://pubmed.ncbi.nlm.nih.gov/35018698
Autor:
Tristan J. Tyler, Thomas Durek, David J. Craik, Simon J. de Veer, Kuok Yap, Fabian B. H. Rehm
Publikováno v:
Journal of the American Chemical Society. 143(46)
Chemoenzymatic protein and peptide modification is a powerful means of generating defined, homogeneous conjugates for a range of applications. However, the use of transpeptidases is limited by the need to prepare synthetic peptide conjugates to be li
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a938ac1332a433043794856a9fc749ab
https://pubmed.ncbi.nlm.nih.gov/34761936
https://pubmed.ncbi.nlm.nih.gov/34761936
