Cross-phyla protein annotation by structural prediction and alignment

Autor: Fabian Ruperti, Nikolaos Papadopoulos, Jacob M. Musser, Milot Mirdita, Martin Steinegger, Detlev Arendt

Publikováno v: Genome Biology, Vol 24, Iss 1, Pp 1-21 (2023)

Abstract Background Protein annotation is a major goal in molecular biology, yet experimentally determined knowledge is typically limited to a few model organisms. In non-model species, the sequence-based prediction of gene orthology can be used to i

Externí odkaz: https://doaj.org/article/13136b8e8a7842468195aa44612402f6

CD3 Target Affinity Chromatography Mass Spectrometry as a New Tool for Function–Structure Characterization of T-Cell Engaging Bispecific Antibody Proteoforms and Product-Related Variants

Autor: Steffen Lippold, Michaela Hook, Christian Spick, Alexander Knaupp, Kevin Whang, Fabian Ruperti, Lance Cadang, Nisana Andersen, Annette Vogt, Michael Grote, Dietmar Reusch, Markus Haberger, Feng Yang, Tilman Schlothauer

Publikováno v: Analytical Chemistry. 95:2260-2268

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ca4f53e3f3a5ec666eb586b8553e20c3
https://doi.org/10.1021/acs.analchem.2c03827

Profiling cellular diversity in sponges informs animal cell type and nervous system evolution

Autor: Jacob M. Musser, Warren R. Francis, Yannick Schwab, Florian Wolf, Paolo Ronchi, Michael Nickel, Roberto Feuda, Jaime Huerta-Cepas, Andrea B. Kohn, Klaske J. Schippers, Cong Liang, Constantin Pape, Maike Renkert, Gleb Bourenkov, Giulia Mizzon, Nicole L. Schieber, Sergio Vargas, Kaia Achim, Gert Wörheide, Detlev Arendt, Anna Kreshuk, Imre Gaspar, Thomas R. Schneider, Maxim Polikarpov, Nikolaos Papadopoulos, Peer Bork, Svenja Kling, Bo Wang, Pawel Burkhardt, Ana Hernández-Plaza, Martin Beck, Alexander J. Tarashansky, Carlos Pérez Cantalapiedra, Fabian Ruperti, Leonid L. Moroz, Leslie Pan, Jörg U. Hammel

Publikováno v: Science
Musser, J.M.; Schippers, K.J.; Nickel, M.; Mizzon, G.; Kohn, A.B.; Pape, C.; Ronchi, P.; Papadopoulos, N.; Tarashansky, A.J.; Hammel, J.U.; Wolf, F.; Liang, C.; Hernández-Plaza, A.; Cantalapiedra, C.P.; Achim, K.; Schieber, N.L.; Pan, L.; Ruperti, F.; Francis, W.R.; Vargas, S.; Kling, S.; Renkert, M.; Polikarpov, M.; Bourenkov, G.; Feuda, R.; Gaspar, I.; Burkhardt, P.; Wang, B.; Bork, P.; Beck, M.; Schneider, T.R.; Kreshuk, A.; Wörheide, G.; Huerta-Cepas, J.; Schwab, Y.; Moroz, L.L.; Arendt, D.: Profiling cellular diversity in sponges informs animal cell type and nervous system evolution. In: Science. Vol. 374 (2021) 6568, 717-723. (DOI: /10.1126/science.abj2949)

Sponges and evolutionary origins Sponges represent our distant animal relatives. They do not have a nervous system but do have a simple body for filter feeding. Surveying the cell types in the freshwater sponge Spongilla lacustris , Musser et al . fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ee9f0a20b7309efbd605db64e92da34

Library Selection with a Randomized Repertoire of (βα) 8 -Barrel Enzymes Results in Unexpected Induction of Gene Expression

Autor: Michael Bott, Oona Jung, Fabian Ruperti, Gerhard Lehmann, Katja Dettmer, Bettina Rohweder, Wolfram Gronwald, Thomas Treiber, Tino Polen, Reinhard Sterner, Mona Linde, Norbert Eichner, Chitra Rajendran, Gunter Meister

Publikováno v: Biochemistry 58(41), 4207-4217 (2019). doi:10.1021/acs.biochem.9b00579

The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::770083ab8e0b3f37ca845c4f5bc26dfe
https://hdl.handle.net/2128/23265