Zobrazeno 1 - 10
of 1 656
pro vyhledávání: '"FLP-FRT recombination"'
Autor:
Adrián Fragoso-Luna, Raquel Romero-Bueno, Michael Eibl, Cristina Ayuso, Celia Muñoz-Jiménez, Vladimir Benes, Ildefonso Cases, Peter Askjaer
Publikováno v:
Genetics. 223
Control of gene expression in specific tissues and/or at certain stages of development allows the study and manipulation of gene function with high precision. Site-specific genome recombination by the flippase (FLP) and cyclization recombination (Cre
Akademický článek
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Autor:
Michael L. Nonet
Publikováno v:
Genetics. 215:903-921
The application of CRISPR technology has greatly facilitated the creation of transgenic Caenorhabditis elegans lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here, I describe a new approach
Autor:
Savannah J. Lusk, Andrew McKinney, Patrick J. Hunt, Paul G. Fahey, Jay Patel, Andersen Chang, Jenny J. Sun, Vena K. Martinez, Ping Jun Zhu, Jeremy R. Egbert, Genevera Allen, Xiaolong Jiang, Benjamin R. Arenkiel, Andreas S. Tolias, Mauro Costa-Mattioli, Russell S. Ray
BackgroundA full understanding of circuits and cellular mechanisms governing health and disease requires the dissection and multi-faceted study of discrete cell subtypes in developing and adult animal models. Recombinase-driven expression of transgen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::161c9054d8b1a9c3b7e74a8a00d75fb1
https://doi.org/10.1101/2021.06.10.447908
https://doi.org/10.1101/2021.06.10.447908
Publikováno v:
Plant Biotechnology Journal
Summary The bacterium Erwinia amylovora, the causal agent of fire blight disease in apple, triggers its infection through the DspA/E effector which interacts with the apple susceptibility protein MdDIPM4. In this work, MdDIPM4 knockout has been produ
Autor:
Yukiko U. Inoue, Junko Asami, Itaru Imayoshi, Takayoshi Inoue, Kazumi Shimaoka, Mayumi Yamada, Eriko Koike, Mikio Hoshino, Mayuko Hotta, Kei Hori, Ryosuke Kaneko, Yuki Morimoto, Shinji Oki
Publikováno v:
Cells
Volume 10
Issue 5
Cells, Vol 10, Iss 1076, p 1076 (2021)
Volume 10
Issue 5
Cells, Vol 10, Iss 1076, p 1076 (2021)
Fluorescent reporter mouse lines and Cre/Flp recombinase driver lines play essential roles in investigating various molecular functions in vivo. Now that applications of the CRISPR/Cas9 genome-editing system to mouse fertilized eggs have drastically
Autor:
Jaime H. Ortega, Beatriz Salas, Sergio A. Álvarez, Fernando Amaya, Carlos A. Santiviago, Camila Valenzuela, Paulina A Fernández, Constanza Morgado, Carolina Rubilar, Víctor Cuevas, Fernando Baisón-Olmo, Gabriel Vera, Marcela Zabner
Publikováno v:
Microorganisms, Vol 9, Iss 475, p 475 (2021)
Microorganisms
Volume 9
Issue 3
Microorganisms
Volume 9
Issue 3
The type III secretion systems (T3SS) encoded in pathogenicity islands SPI-1 and SPI-2 are key virulence factors of Salmonella. These systems translocate proteins known as effectors into eukaryotic cells during infection. To characterize the function
Autor:
Masayuki Sakamoto, Masatoshi Inoue, Atsuya Takeuchi, Shigetaka Kobari, Tatsushi Yokoyama, Shin-ichiro Horigane, Sayaka Takemoto-Kimura, Manabu Abe, Kenji Sakimura, Masanobu Kano, Kazuo Kitamura, Hajime Fujii, Haruhiko Bito
Publikováno v:
Cell Reports Methods. 2:100168
Genetically encoded calcium indicators (GECIs) are widely used to measure calcium transients in neuronal somata and processes, and their use enables the determination of action potential temporal series in a large population of neurons. Here, we gene
Akademický článek
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Autor:
Drew Friedmann, David A. Stafford, Angus Y. Lee, Daniel J. Kramer, Dirk Hockemeyer, Liqun Luo, John Ngai, Polina Kosillo, Helen S. Bateup
Dopamine neurons project to diverse regions throughout the brain to modulate various brain processes and behaviors. It is increasingly appreciated that dopamine neurons are heterogeneous in their gene expression, circuitry, physiology, and function.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8b7f1e0c1104692663770df234003581
https://doi.org/10.1101/2020.06.24.167908
https://doi.org/10.1101/2020.06.24.167908