Zobrazeno 1 - 10
of 64
pro vyhledávání: '"F.X. Wilhelm"'
Autor:
Adalbert F.X. Wilhelm, Hans A. Kestler
This book offers a snapshot of the state-of-the-art in classification at the interface between statistics, computer science and application fields. The contributions span a broad spectrum, from theoretical developments to practical applications; they
Autor:
Marcelle Wilhelm, F.X. Wilhelm
Publikováno v:
Eukaryotic Cell
Eukaryotic Cell, American Society for Microbiology, 2006, 5 (10), pp.1760-9. ⟨10.1128/EC.00159-06⟩
Eukaryotic Cell, American Society for Microbiology, 2006, 5 (10), pp.1760-9. ⟨10.1128/EC.00159-06⟩
Reverse transcriptase (RT) and integrase (IN) play a central role in the replication and transposition of retroelements. Increasing evidence suggests that the interaction between these two enzymes is functional and plays an important role in replicat
Publikováno v:
The EMBO Journal. 21:4368-4379
LTR-retrotransposons are abundant components of all eukaryotic genomes and appear to be key players in their evolution. They share with retroviruses a reverse transcription step during their replication cycle. To better understand the replication of
Publikováno v:
Nucleic Acids Research. 29:2217-2222
The fidelity of the yeast retrotransposon Ty1 reverse transcriptase (RT) was determined by an assay based on gel electrophoresis. Steady-state kinetics analyses of deoxyribonucleotide (dNTP) incorporation at a defined primer-template site indicate th
Publikováno v:
Biochemical Journal. 348:337-342
Replication of the Saccharomyces cerevisiae Ty1 retrotransposon requires a reverse transcriptase capable of synthesizing Ty1 DNA. The first description of an active form of a recombinant Ty1 enzyme with polymerase and RNase H activities is reported h
Publikováno v:
Journal of Molecular Biology. 288:505-510
Replication of the yeast Ty1 retrotransposon occurs by a mechanism similar to that of retroviruses. According to the current model of retroviral reverse transcription, two strand transfers (the so-called minus-strand and plus-strand strong-stop DNA t
Publikováno v:
Yeast
Yeast, Wiley, 1997, 13 (7), pp.639-45. ⟨10.1002/(SICI)1097-0061(19970615)13:73.0.CO;2-Y⟩
Yeast, Wiley, 1997, 13 (7), pp.639-45. ⟨10.1002/(SICI)1097-0061(19970615)13:73.0.CO;2-Y⟩
In the reverse transcription initiation complex of the yeast Ty1 retrotransposon, interaction between the template RNA and primer tRNAiMet is not limited to base pairing of the primer binding site (PBS) with ten nucleotides at the 3' end of tRNAiMet,
Publikováno v:
Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 1997, 25 (11), pp.2161-6
Nucleic Acids Research, Oxford University Press, 1997, 25 (11), pp.2161-6
A specific terminal structure of preintegrative DNA is required for transposition of retroviruses and LTR-retrotransposons. We have used an anchored PCR technique to map the 3'ends of DNA intermediates synthesized inside yeast Ty1 and Ty3 retrotransp
Publikováno v:
Nucleic Acids Research. 22:4560-4565
Reverse transcription of the yeast retrotransposon Ty1 is primed by the cytoplasmic initiator methionine tRNA (tRNA(iMet)). The primer tRNA(iMet) is packaged inside virus-like particles (VLPs) and binds to a 10 nucleotides minus-strand primer binding
Publikováno v:
Nucleic Acids Research. 20:791-796
The expression of mutant tyrosine-inserting ochre suppressor SUP4-o tRNA genes in vivo in S. cerevisiae was examined as a basis for further studies of tRNA transcription and processing. In vivo yeast precursor tRNAs have been identified by filter hyb