Zobrazeno 1 - 10
of 130
pro vyhledávání: '"F.P. Ottensmeyer"'
Publikováno v:
Journal of Structural Biology. 113:35-46
The 54-kDa subunit SRP54 of the signal recognition particle in eukaryotic cells is responsible for the recognition of nascent proteins destined for secretion or membrane integration. The three-dimensional structure of this protein was determined usin
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 54:424-425
One of the main applications of an energy filter is to obtain elemental distribution images by electron spectroscopic imaging. This technique has been pushed to the imaging of fine structure such as the sp2 and sp3 states of carbon at a sub-nanometer
Autor:
N.A. Farrow, F.P. Ottensmeyer
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 50:1058-1059
Electron microscopy produces 2D images of 3D objects with resolutions generally from about 2-5 nm for stained or shadowed specimens, to as good as 0.3-0.5 nm for unstained specimens using bright field or dark field techniques. Many groups have worked
Autor:
F.P. Ottensmeyer, B.W. Frankland
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 45:84-87
Over the years biology and materials science have profited greatly from the imaging potential of electron microscopy for structural and morphological analysis. Gross structures, grains, subgrains or subcellular organelles, mitochondria and the Golgi
Publikováno v:
Ultramicroscopy. 3:191-202
The effect of radiation exposure on visual recognition of individual macromolecules of the protein protamine was examined using high resolution electron microscopy in scanning transmission, fixed beam transmission, dark field and bright field, and re
Autor:
George Harauz, F.P. Ottensmeyer
Publikováno v:
Ultramicroscopy. 12:309-319
An algorithm is presented for the direct reconstruction of a three-dimensional image from two-dimensional projections with directions randomly distributed over the unit sphere. An iterative algorithm for improving the rotational alignment of projecti
Publikováno v:
Proceedings, annual meeting, Electron Microscopy Society of America. 36:147-159
The electron microscopic extraction of information from biological specimens has always been a compromise among at least four factors: resolution, contrast, radiation damage and signal-to-noise ratio. To overcome the notoriously low contrast of most
Publikováno v:
Proceedings of the National Academy of Sciences. 82:785-789
The signal recognition particle (SRP) is a ribonucleoprotein consisting of six distinct polypeptide components and one molecule of small cytoplasmic RNA (7SL RNA). The particle was previously shown to function in protein translocation across and prot
Autor:
F.P. Ottensmeyer, R.A. Ploc
Publikováno v:
Micron (1969). 6:65-72
A prism-mirror-prism energy filter installed in a transmission electron microscope was used to examine oxide films formed on zirconium. Bright and dark-field images were obtained using electrons which were elastically and inelastically scattered. Rep
Autor:
F.P. Ottensmeyer
Publikováno v:
Journal of Ultrastructure Research. 88:121-134
A new imaging modality in electron microscopy uses energy filtration to produce micrographs with elastically scattered electrons or with electrons that have lost a specific, often characteristic amount of energy in interacting with the specimen. No d