Measuring DNA modifications with the comet assay: a compendium of protocols

Autor: Andrew Collins, Peter Møller, Goran Gajski, Soňa Vodenková, Abdulhadi Abdulwahed, Diana Anderson, Ezgi Eyluel Bankoglu, Stefano Bonassi, Elisa Boutet-Robinet, Gunnar Brunborg, Christy Chao, Marcus S. Cooke, Carla Costa, Solange Costa, Alok Dhawan, Joaquin de Lapuente, Cristian Del Bo’, Julien Dubus, Maria Dusinska, Susan J. Duthie, Naouale El Yamani, Bevin Engelward, Isabel Gaivão, Lisa Giovannelli, Roger Godschalk, Sofia Guilherme, Kristine B. Gutzkow, Khaled Habas, Alba Hernández, Oscar Herrero, Marina Isidori, Awadhesh N. Jha, Siegfried Knasmüller, Ingeborg M. Kooter, Gudrun Koppen, Marcin Kruszewski, Carina Ladeira, Blanca Laffon, Marcelo Larramendy, Ludovic Le Hégarat, Angélique Lewies, Anna Lewinska, Guillermo E. Liwszyc, Adela López de Cerain, Mugimane Manjanatha, Ricard Marcos, Mirta Milić, Vanessa Moraes de Andrade, Massimo Moretti, Damian Muruzabal, Matjaž Novak, Rui Oliveira, Ann-Karin Olsen, Norah Owiti, Mário Pacheco, Alok K. Pandey, Stefan Pfuhler, Bertrand Pourrut, Kerstin Reisinger, Emilio Rojas, Elise Rundén-Pran, Julen Sanz-Serrano, Sergey Shaposhnikov, Ville Sipinen, Karen Smeets, Helga Stopper, João Paulo Teixeira, Vanessa Valdiglesias, Mahara Valverde, Frederique van Acker, Frederik-Jan van Schooten, Marie Vasquez, Johannes F. Wentzel, Maciej Wnuk, Annelies Wouters, Bojana Žegura, Tomas Zikmund, Sabine A. S. Langie, Amaya Azqueta

Publikováno v: Nature Protocols
Nature Protocols, 2023, ⟨10.1038/s41596-022-00754-y⟩
Nature Protocols. Nature Publishing Group
Nat Protoc

The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b143a2dbb2770d10bfa61ac51dd6ffd
https://doi.org/10.1038/s41596-022-00754-y

Sequence analysis of cell-free DNA derived from cultured human bone osteosarcoma (143B) cells

Autor: Abel Jacobus Bronkhorst, Johannes F Wentzel, Vida Ungerer, Dimetrie L Peters, Janine Aucamp, Etienne Pierre de Villiers, Stefan Holdenrieder, Piet J Pretorius

Publikováno v: Tumor Biology, Vol 40 (2018)

The true importance of cell-free DNA in human biology, together with the potential scale of its clinical utility, is tarnished by a lack of understanding of its composition and origin. In investigating the cell-free DNA present in the growth medium o

Externí odkaz: https://doaj.org/article/97cf857cfc46473ca7562642d2ea3b1a

Anti-melanoma activities of artemisone and prenylated amino-artemisinins in combination with known anticancer drugs

Autor: Richard K. Haynes, Joe M. Viljoen, Johannes F. Wentzel, Angélique Lewies, Ho Ning Wong, Lissinda H. Du Plessis, Michaela Haigh

Publikováno v: Frontiers in Pharmacology
Frontiers in Pharmacology, Vol 11 (2020)

The most frequently occurring cancers are those of the skin, with melanoma being the leading cause of death due to skin cancer. Breakthroughs in chemotherapy have been achieved in certain cases, though only marginal advances have been made in treatme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4da2640b3925f551891ffa54635e4b6a
https://hdl.handle.net/10394/36097

Lewies, A., et al. The Potential Use of Natural and Structural Analogues of Antimicrobial Peptides in the Fight against Neglected Tropical Diseases. Molecules 2015, 20, 15392–15433

Autor: Angélique Lewies, Johannes F. Wentzel, Garmi Jacobs, Lissinda H. Du Plessis

Publikováno v: Molecules, Vol 20, Iss 9, Pp 16757-16757 (2015)

The authors wish to make the following corrections to this paper [1]: [...]

Externí odkaz: https://doaj.org/article/e2dece7ab8c4406683e805a65457c1d5

Evaluation of the cytotoxic properties, gene expression profiles and secondary signalling responses of cultured cells exposed to fumonisin B1, deoxynivalenol and zearalenone mycotoxins

Autor: Johannes F. Wentzel, Martani J Lombard, Lizelle Zandberg, Lissinda H. Du Plessis

Publikováno v: Archives of Toxicology. 91:2265-2282

Mycotoxins are toxic secondary metabolites produced by a range of fungi and are common contaminants of agricultural crops. These toxins are chemically diverse and structurally stable, enabling them to enter the food chain which can lead to numerous a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5eb7e3422e4f7a005027e68c172ec4f2
https://doi.org/10.1007/s00204-016-1872-y