Zobrazeno 1 - 10
of 10
pro vyhledávání: '"F. T. Kear"'
Autor:
Debra Steplock, F. T. Kear, Edward J. Weinman, Anna V.P. Santos, Jianfei Ma, A. A. Bernardo, R. Brooks Robey
Publikováno v:
Journal of Clinical Investigation. 104:195-201
In the renal proximal tubule, the activities of the basolateral Na+/HCO3– cotransporter (NBC) and the apical Na+/H+ exchanger (NHE3) uniformly vary in parallel, suggesting that they are coordinately regulated. PKA-mediated inhibition of NHE3 is med
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26b01fda2cf95e6f848468d29ded663e
https://doi.org/10.1172/jci5344
https://doi.org/10.1172/jci5344
Autor:
Long Jiang Wang, F. T. Kear, Anna V.P. Santos, Jianfei Ma, Jose A.L. Arruda, Ofelia S. Ruiz, R.B. Robey, A. A. Bernardo, C.-J. Li
Publikováno v:
Biochemistry. 37:9894-9901
The green fluorescent protein of Aequorea victoria (GFP) is a natural peptide chromophore without substrate or cofactor requirements for fluorescence. In vitro, a recombinant F64L/S65T GFP mutant (GFPmut1) exhibited pH sensitive fluorescence within t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::600e05726692b75404d8f2812cbf5612
https://doi.org/10.1021/bi980857x
https://doi.org/10.1021/bi980857x
Publikováno v:
Journal of Membrane Biology. 158:49-57
The Na+/HCO3- cotransporter is the main system that mediates bicarbonate removal out of the proximal tubule cell into the blood. We have previously partially purified this protein and showed that chemical modification of the alpha-amino groups by flu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75050065d2668b6eeb4bf188390f7be9
https://doi.org/10.1007/s002329900242
https://doi.org/10.1007/s002329900242
Publikováno v:
Experimental Biology and Medicine. 198:846-851
The Na-H antiporter of renal-brush border membranes is inhibited by cyclic AMP and stimulated by protein kinase C. The proximal tubule contains guanylate cyclase and is capable of cyclic GMP production. The effect of cGMP on renal Na-H antiporter act
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::093c0b1879dfc058184569046d7eaca3
https://doi.org/10.3181/00379727-198-43323
https://doi.org/10.3181/00379727-198-43323
Autor:
Doris Joy Espiritu, Jose A.L. Arruda, F. T. Kear, V.C. Ibañez, Oscar Noboa, R.B. Robey, Ofelia S. Ruiz, A. A. Bernardo
Publikováno v:
The Journal of membrane biology. 187(2)
Angiotensin II (AII) plays an important role in renal proximal tubular acidification via the costimulation of basolateral Na/HCO3 cotransporter (NBC) and apical Na/H exchanger (NHE) activities. These effects are mediated by specific G protein-coupled
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a74bbd00e0efc43ea97bf5300d6cda7
https://pubmed.ncbi.nlm.nih.gov/12029370
https://pubmed.ncbi.nlm.nih.gov/12029370
Publikováno v:
The Journal of membrane biology. 154(2)
We have previously partially purified the basolateral Na+/HCO− 3 cotransporter from rabbit renal cortex and this resulted in a 400-fold purification, and an SDS-PAGE analysis showed an enhancement of a protein band with a MW of approximately 56 kDa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdb61a7ea9f7bf9f10555d8800c6ee26
https://pubmed.ncbi.nlm.nih.gov/8929289
https://pubmed.ncbi.nlm.nih.gov/8929289
Autor:
F. T. Kear, J. A. Stim, Jose A.L. Arruda, Ofelia S. Ruiz, A. A. Bernardo, Yi-Yong Qiu, H. Weidman
Publikováno v:
The Journal of Membrane Biology. 145
The activity of the Na-H antiporter is inhibited by cyclic AMP-dependent protein kinase A (cAMP.PKA). The inhibitory effect of PKA on the Na-H antiporter is mediated through a regulatory protein that can be dissociated from the antiporter by limited
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8e42a850b43cedbfa011a7bec076c36
https://doi.org/10.1007/bf00233307
https://doi.org/10.1007/bf00233307
Publikováno v:
The Journal of membrane biology. 140(1)
Fluorescein isothiocyanate (FITC) fluorescently labels amino groups and has been useful in detecting conformational changes in transport proteins through quenching or enhancement of the fluorescence signal upon exposure of protein to substrates. Solu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::923b9ab2465b2ad84f0f4045482e2b16
https://pubmed.ncbi.nlm.nih.gov/8051692
https://pubmed.ncbi.nlm.nih.gov/8051692
Publikováno v:
The Journal of Membrane Biology. 140
The renal basolateral Na+/HCO3- cotransporter is the main system responsible for HCO3- transport from proximal tubule cells into the blood. The present study was aimed at purifying and functionally reconstituting the Na+/HCO3- cotransporter protein f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::212374d65e4528766914ecbe44d04036
https://doi.org/10.1007/bf00234483
https://doi.org/10.1007/bf00234483
Publikováno v:
Kidney International. (3):696-704
Regulation of the Na-HCO 3 cotransporter: VI. Mechanism of the stimulatory effect of protein kinase C. We have previously shown that the activity of the Na-HCO 3 cotransporter is stimulated by protein kinase C (PKC) activation, but the mechanism resp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5db04e93cb25cb140848cfb9c0793ab0