Výsledky vyhledávání - "F. Kaprálek"

Effects of temperature and novobiocin on the expression of calf prochymosin gene and on plasmid copy number in recombinantEscherichia coli

Autor: Milan Fábry, Juraj Sedlacek, Pavel Tichý, F. Kaprálek

Publikováno v: Folia Microbiologica. 43:63-67

Escherichia coli strain HB101 harboring an expression plasmid bearing calf prochymosin gene under the control of the tac promoter was grown in the presence of IPTG with or without novobiocin at 28 and 40 degrees C, respectively. The differential rate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b43a7fa4e9d42c053b76476a924924e2
https://doi.org/10.1007/bf02815545

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Effect of benzyl alcohol and ethanol on cold-shock response ofBacillus subtilis

Autor: P. Svoboda, Ivo Konopásek, F. Kaprálek, Jaroslava Svobodová

Publikováno v: Folia Microbiologica. 40:633-638

Benzyl alcohol and ethanol were found to exhibit an opposite effect on cold adaptation ofBacillus subtilis. The growth lag induced in bacteria by temperature shift-down was reduced in the presence of benzyl alcohol from 80 to 58 min, whereas ethanol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7177aa3ff8f406d07e7747ffbccfe7be
https://doi.org/10.1007/bf02818521

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The structural stability of an expression plasmid bearing a heterologous cloned gene depends on whether this gene is expressed or not

Autor: P. Ječmen, F. Kaprálek

Publikováno v: Biotechnology Letters. 14:251-256

During subculturing of recombinant Escherichia coli under conditions blocking cloned gene expression the plasmid was structurally stable for 200 generations. However, under conditions permitting foreign gene expression only 10% of plasmid containing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::961e23b8293ff8eff6191ced7f1784fc
https://doi.org/10.1007/bf01022319

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Isolation, biochemical characterization and crystallization of the p15gag proteinase of myeloblastosis associated virus expressed in E. coli

Autor: Vladimír Kostka, F. Kaprálek, Iva Pichová, Juraj Sedlacek, Vladimir Benes, S. Foundling, Petr Štrop, Milan Souček, L. Pavlíčková, M. Trávníček

Publikováno v: International Journal of Biochemistry. 24:235-242

1. 1. The p15 gag proteinase responsible for the processing of the polyprotein precursor of the myeloblastosis associated virus was obtained by a recombinant technique in an E. coli expression system. The massive expression of the intentionally trunc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32638250d961ee70c177e70030849e18
https://doi.org/10.1016/0020-711x(92)90252-v

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Fermentation conditions for high-level expression of thetac-promoter-controlled calf prochymosin cDNA inEscherichia coliHB101

Autor: P. Ječmen, Stanislav Zadražil, F. Kaprálek, J. Sedláček, Milan Fábry

Publikováno v: Biotechnology and Bioengineering. 37:71-79

Escherichia coli HB101 harboring an expression plasmid that bears the calf prochymosin gene controlled by the tac promoter was cultivated under different conditions in order to find an optimal fermentation arrangement that would lead to maximal proch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5e5aa33d14a60f9d325d5de3127ba2a1
https://doi.org/10.1002/bit.260370111

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Fermentation conditions for high-level expression of the tac-promoter-controlled calf prochymosin cDNA in Escherichia coli HB101

Autor: F, Kaprálek, P, Jecmen, J, Sedlácek, M, Fábry, S, Zadrazil

Publikováno v: Biotechnology and bioengineering. 37(1)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::151274ce8cbc9142290ffb285b4156dc
https://pubmed.ncbi.nlm.nih.gov/18597309

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p15gag proteinase of myeloblastosis associated virus: specificity studies with substrate based inhibitors

Autor: P, Strop, L, Pavlícková, D, Stys, M, Soucek, J, Urban, O, Hrusková, F, Kaprálek, P, Jecmen, J, Sedlácek, V, Kostka

Publikováno v: Advances in experimental medicine and biology. 306

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8904700da6ec9f8e7170d2a93340980f
https://pubmed.ncbi.nlm.nih.gov/1812752

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p15 gag Proteinase of Myeloblastosis Associated Virus: Specificity Studies with Substrate Based Inhibitors

Autor: P. Štrop, L. Pavlíčková, D. Štys, M. Souček, J. Urban, O. Hrušková, F. Kaprálek, P. Ječmen, J. Sedláček, V. Kostka

Publikováno v: Advances in Experimental Medicine and Biology ISBN: 9781468460148

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c32d7d52c6d43e2da08f43d5afea5ad5
https://doi.org/10.1007/978-1-4684-6012-4_69

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Physiological characteristics of chemostatically grownCitrobacter freundii as a function of the specific growth rate and type of nutrient limitation

Autor: F. Kaprálek, J Dolezal

Publikováno v: Folia Microbiologica. 21:168-177

Citrobacter freundii was grown aerobically in a chemostat on a mineral medium with galactose or glucose as carbon and energy sources under limitation by carbon or nitrogen source respectively. At various specific growth rates ranging from 7 to 95% mu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18e3191a9d35987499e8121dfe7dd540
https://doi.org/10.1007/bf02883152

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