Zobrazeno 1 - 10
of 155
pro vyhledávání: '"F. Kalousek"'
Autor:
J. Adamec, F. Kalousek
Publikováno v:
Folia microbiologica. 44(1)
Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe here two-step PCR-based method for generating single or multiple mutations, insertions and d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::036af5ed3d5b046b8f68355bb6a08b61
https://pubmed.ncbi.nlm.nih.gov/10489690
https://pubmed.ncbi.nlm.nih.gov/10489690
Autor:
G, Isaya, F, Kalousek
Publikováno v:
Methods in enzymology. 248
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2c21600df9d1dd52013133ac9c45fa4f
https://pubmed.ncbi.nlm.nih.gov/7674944
https://pubmed.ncbi.nlm.nih.gov/7674944
Publikováno v:
The Journal of biological chemistry. 269(12)
Most nuclearly encoded mitochondrial proteins are synthesized with an amino-terminal leader peptide that is cleaved by the mitochondrial processing peptidase (MPP). Purified rat liver MPP, like the Neurospora and yeast enzymes, consists of two nonide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0c21304fb1b64602295178782f8d98f9
https://pubmed.ncbi.nlm.nih.gov/8132665
https://pubmed.ncbi.nlm.nih.gov/8132665
Publikováno v:
The Journal of biological chemistry. 268(10)
When purified rat liver ornithine transcarbamylase (OTC), a trimer of 36 kDa subunits, was denatured in 6 M guanidine hydrochloride and then diluted 50-100-fold, no activity was recovered, and the OTC subunits aggregated. In contrast, when the chaper
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::94414f1872e2f1af256fdbdc86936c67
https://pubmed.ncbi.nlm.nih.gov/8096512
https://pubmed.ncbi.nlm.nih.gov/8096512
Publikováno v:
Biochemical Society Transactions. 28:A350-A350
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e49394063292bc4130944c64b332def
https://doi.org/10.1042/bst028a350c
https://doi.org/10.1042/bst028a350c
Publikováno v:
Journal of Biological Chemistry. 253:3939-3944
We report experiments describing the isolation and characterization of ornithine transcarbamylase from normal human liver. Our preparative procedure employs initial centrifugation and heat steps, intermediate batch-wise adsorption and desorption from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ce27cf5f0aad0971e82da2623bd98fc0
https://doi.org/10.1016/s0021-9258(17)34781-6
https://doi.org/10.1016/s0021-9258(17)34781-6
Publikováno v:
Journal of Biological Chemistry. 255:60-65
We have purified propionyl-CoA carboxylase from normal, postmortem human liver to homogeneity. The isolation procedure, which provided an approximately 3000-fold purification and an overall yield of 26%, employed initial centrifugation of a cetyltrim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f81bbcf9a9e21abc7a70fd994ad72bb0
https://doi.org/10.1016/s0021-9258(19)86263-4
https://doi.org/10.1016/s0021-9258(19)86263-4
Autor:
N R Morris, F Kalousek
Publikováno v:
Journal of Biological Chemistry. 244:1157-1163
An enzyme has been prepared from rat spleen which catalyzes the transfer of methyl groups from S-adenosylmethionine to cytosine moieties in DNA. This enzyme, which has been purified about 200-fold from the crude homogenate, differs from the known bac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8dde54c4d4f29665f6dc720055a170cd
https://doi.org/10.1016/s0021-9258(18)91823-5
https://doi.org/10.1016/s0021-9258(18)91823-5
Publikováno v:
Collection of Czechoslovak Chemical Communications. 31:2794-2802
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5884e17d906567b51bfb8dfb5b334aa2
https://doi.org/10.1135/cccc19662794
https://doi.org/10.1135/cccc19662794
Publikováno v:
Journal of Molecular Biology. 55:277-281
Native ribosomal subunits separated by sucrose gradient centrifugation are able to associate. The particles so formed sediment between 59 s and 63 s (designated 61 s ribosomes). In contrast, derived subunits associate to form 70 s ribosomes. The 50 s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c77f688c247b5733ce4b0744bccaab1
https://doi.org/10.1016/0022-2836(71)90198-7
https://doi.org/10.1016/0022-2836(71)90198-7