Výsledky vyhledávání - "F. B. Rayment"

Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies

Autor: J E Rowedder, C A Henwood, Jeffrey J. Clare, M A Romanos, Koti Tatachar Sreekrishna, Marie-Louise Smith, M M Payne, F. B. Rayment

Publikováno v: Gene. 105:205-212

We have constructed a synthetic secretion cassette encoding the alpha-factor prepro leader peptide from Saccharomyces cerevisiae fused to mouse epidermal growth factor (mEGF). This was used to compare the secretion of mEGF, a 53-amino acid polypeptid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3c2c235bf903417735141f43f849b5f
https://doi.org/10.1016/0378-1119(91)90152-2

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Expression of tetanus toxin fragment C in yeast: gene synthesis is required to eliminate fortuitous polyadenylation sites in AT-rich DNA

Autor: F. B. Rayment, Mike M. Payne, Andrew Makoff, Neil F. Fairweather, Michael A. Romanos, Debbie E. Slater, Katrina M. Beesley, Jeffrey J. Clare

Publikováno v: Nucleic Acids Research. 19:1461-1467

Fragment C is a non-toxic 50 kDa fragment of tetanus toxin which is a candidate subunit vaccine against tetanus. The AT-rich Clostridium tetani DNA encoding fragment C could not be expressed in Saccharomyces cerevisiae due to the presence of several

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f62189e0936df51ef023945b9fb6e6ae
https://doi.org/10.1093/nar/19.7.1461

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The production of human parvovirus capsid proteins in Escherichia coli and their potential as diagnostic antigens

Autor: E. Crosdale, D. J. Morris, Jeffrey J. Clare, F. B. Rayment, P. Talbot, J. R. Pattison

Publikováno v: Journal of General Virology. 71:2665-2672

We have expressed a number of polypeptides derived from the capsid proteins of the human parvovirus B19 in Escherichia coli. These include native VP1 (84K) and VP2 (58K) proteins and also fusions to beta-galactosidase containing differing amounts of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::076a76139026249a2f182d9a00c26370
https://doi.org/10.1099/0022-1317-71-11-2665

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Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high-level production and immunological properties

Autor: A. J. Makoff, Katrina M. Beesley, Ian G. Charles, Michael A. Romanos, F. B. Rayment, Neil F. Fairweather, Gordon Dougan, Jeffrey J. Clare, Stuart P. Ballantine

Acellular whooping cough vaccines are based on pertussis toxoid but their effectiveness may be increased by the addition of other Bordetella pertussis antigens. We expressed the immunogenic outer membrane protein pertactin (P69) from B. pertussis to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39612c64ae4afe3677644c21ffb98159
https://hdl.handle.net/10453/13749

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High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene

Autor: Jeffrey J. Clare, Stuart P. Ballantine, Koti Tatachar Sreekrishna, F. B. Rayment, M A Romanos

Publikováno v: Bio/technology (Nature Publishing Company). 9(5)

We have used the methylotrophic yeast, Pichia pastoris, to express high levels of tetanus toxin fragment C, a potential subunit vaccine against tetanus. In high biomass fermentations fragment C was induced to 27% of total cell protein or about 12 g/l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af9a8de29d012972c26e0b1d56607319
https://pubmed.ncbi.nlm.nih.gov/1367310

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