Online capillary liquid-liquid electroextraction of peptides as fast pre-concentration prior to LC-MS

Autor: Thomas Hankemeier, Petrus W. Lindenburg, F. W. Alexander Tempels, Jan van der Greef, Remco Seitzinger, U.R. Tjaden

Publikováno v: Electrophoresis, 23-24, 31, 3903-3912

In this research paper, we show that capillary electroextraction (cEE) is capable of fast online peptide concentration and that it can be coupled online to LC-MS to result in a fast and sensitive method. Electroextraction takes place when an electric

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e88889864f2ea1ef9847a57ad69f170b
https://doi.org/10.1002/elps.201000347

On-line coupling of SPE and CE-MS for peptide analysis

Autor: Gerhardus J. de Jong, F. W. Alexander Tempels, Willy J. M. Underberg, Govert W. Somsen

Publikováno v: Electrophoresis, 28(9), 1319-1326. Wiley-VCH Verlag
Tempels, F W A, Underberg, W J M, Somsen, G W & de Jong, G J 2007, '
coupling of SPE and CE-MS for peptide analysis ', Electrophoresis, vol. 28, no. 9, pp. 1319-1326 . https://doi.org/10.1002/elps.200600403

An on-line SPE-CE-MS system has been developed for the analysis of peptides. Analytes are preconcentrated using a C(18) microcolumn (5 x 0.5 mm id), and then introduced into the CE system via a valve interface. The CE system with a Polybrene-poly(vin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::389416c5a7740a045670eff6c80ddc77
https://hdl.handle.net/1871.1/2eb1570c-8035-4106-91a9-bbc8e738c6a9

Molecular interactions of glycopeptide antibiotics investigated by affinity capillary electrophoresis and bioaffinity electrospray ionization‐mass spectrometry

Autor: F. W. Alexander Tempels, J. Jantina Kettenes‐van den Bosch, Pauline J. Vollmerhaus, Albert J. R. Heck

Publikováno v: ELECTROPHORESIS. 23:868-879

Many analytical approaches are available to evaluate (bio)molecular interactions, all of which have their particular advantages and disadvantages. In recent years, two relatively new techniques have emerged that may be used by the bioanalytical commu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::17d86b30012eee0d15229c3d63b41867
https://doi.org/10.1002/1522-2683(200203)23:6<868::aid-elps868>3.0.co

Use of large-volume sample stacking in on-line solid-phase extraction-capillary electrophoresis for improved sensitivity

Autor: Gerhardaus J. de Jong, Carme Aguilar, Marta Calull, F. W. Alexander Tempels, Francesc Borrull, Patricia Puig, Govert W. Somsen

Publikováno v: Puig, P, Tempels, F W A, Somsen, G W, de Jong, G J, Borrull, F, Aguilar, C & Calull, M 2008, ' Use of large-volume sample stacking in
solid-phase extraction-capillary electrophoresis for improved sensitivity ', Electrophoresis, vol. 29, no. 6, pp. 1339-1346 . https://doi.org/10.1002/elps.200700636
Electrophoresis, 29(6), 1339-1346. Wiley-VCH Verlag

We present a new system for the sensitive analysis of cephalosporins by CE using both on-line SPE and large-volume sample stacking (LVSS). Sample volumes of 250 muL were loaded onto the SPE microcolumn which was then desorbed with 426 nL of ACN. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b205c81d1b52ffa6bab933c52297baf
https://pubmed.ncbi.nlm.nih.gov/18288781

Coupling of sequential injection analysis and capillary electrophoresis - Laser-induced fluorescence via a valve interface for on-line derivatization and analysis of amino acids and peptides

Autor: Georgios Theodoridis, Willy J. M. Underberg, Constantinos K. Zacharis, Gerhardus J. de Jong, F. W. Alexander Tempels, Anastasios Voulgaropoulos, Govert W. Somsen

Publikováno v: Journal of Chromatography A, 1132(1-2), 297-303. Elsevier
Zacharis, C K, Tempels, F W A, Theodoridis, G A, Voulgaropoulos, A N, Underberg, W J M, Somsen, G W & de Jong, G J 2006, ' Coupling of sequential injection analysis and capillary electrophoresis-Laser-induced fluorescence via a valve interface for
derivatization and analysis of amino acids and peptides ', Journal of Chromatography A, vol. 1132, no. 1-2, pp. 297-303 . https://doi.org/10.1016/j.chroma.2006.07.076

The on-line coupling of sequential injection analysis (SIA) and capillary electrophoresis (CE) via an in-line injection valve is presented. The SIA system is used for automated derivatization of amino acids and peptides. Dichlorotriazinylaminofluores

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d55510df14693e2e79371f1b7bfc31b9
https://pubmed.ncbi.nlm.nih.gov/16919652