Zobrazeno 1 - 10
of 56
pro vyhledávání: '"F W, McLafferty"'
Autor:
F W McLafferty, M W Senko
Publikováno v:
Annual Review of Biophysics and Biomolecular Structure. 23:763-785
CONTENTS PE RSPEC TIVES A ND OVE RV IEW . . . .. . . . . .. . . . .. . . . . . . . . . . . .. . .. . .. . .. 763 The Sample Vaporization Problem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 764 NEW IO NIZA TIO N ME THODS
Publikováno v:
The Journal of biological chemistry. 276(17)
Phosphopantothenoylcysteine synthase catalyzes the formation of (R)-4'-phospho-N-pantothenoylcysteine from 4'-phosphopantothenate and l-cysteine: this enzyme, involved in the biosynthesis of coenzyme A (CoA), has not previously been identified. Recen
Publikováno v:
Journal of the American Society for Mass Spectrometry. 11(4)
Here a fully automated computer algorithm is applied to complex mass spectra of peptides and proteins. This method uses a subtractive peak finding routine to locate possible isotopic clusters in the spectrum, subjecting these to a combination of the
Publikováno v:
Science (New York, N.Y.). 284(5418)
Autor:
S V, Taylor, N L, Kelleher, C, Kinsland, H J, Chiu, C A, Costello, A D, Backstrom, F W, McLafferty, T P, Begley
Publikováno v:
The Journal of biological chemistry. 273(26)
ThiFSGH and ThiI are required for the biosynthesis of the thiazole moiety of thiamin in Escherichia coli. The overproduction, purification, and characterization of ThiFS and the identification of two of the early steps in the biosynthesis of the thia
Publikováno v:
The Journal of biological chemistry. 272(51)
A widely used procedure for site localization of covalent protein modifications involves proteolysis, partial chromatographic separation of the resulting complex mixture, and tandem mass spectrometry (MS/MS) to identify peptides whose molecular weigh
Publikováno v:
Journal of mass spectrometry : JMS. 31(5)
For high-resolution Fourier transform mass spectrometry of electrosprayed proteins, the signal-to-noise ratio of measuring nozzle-skimmer fragment ions can be improved substantially by their broadband quadrupolar axialization (QA), even without incre
Publikováno v:
The Journal of biological chemistry. 271(7)
Thiaminase I (EC 2.5.1.2) catalyzes the replacement of the thiazole moiety of thiamin with a wide variety of nucleophiles. Here we report the sequencing of a thiaminase I clone from Bacillus thiaminolyticus, the overexpression of the cloned gene in E
Autor:
G A, Valaskovic, F W, McLafferty
Publikováno v:
Rapid communications in mass spectrometry : RCM. 10(7)
Two previously unreported sources of systematic error in electrokinetic injection caused by induced (hydrodynamic) flow in sheathless capillary electrophoresis/electrospray have been characterized for an interface constructed from 5 microns i.d. capi
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 92(6)
Electrospray ionization with Fourier-transform mass spectrometry achieves accurate (< 50-ppm) determination of molecular weights of nucleotides, verifying structures of biological RNA and synthetic single-stranded DNA. High (1o(5)) resolving power ma