Zobrazeno 1 - 10
of 12
pro vyhledávání: '"F M, Wurm"'
Publikováno v:
Cytotechnology. 35(3)
Aim of this study was to identify optimal conditions for suspension transfection in the absence of serum. Transfection parameters for suspension culture can be very different to ones in adherent cells. Most transfection protocols have been developed
Publikováno v:
Cytotechnology. 35(3)
DNA extracted and purified for vaccination, gene therapy or transfection of cultured cells has to meet different criteria. We describe herein, a scalable process for the primary extraction of plasmid DNA suitable for transient expression of recombina
Publikováno v:
Animal Cell Technology: Products from Cells, Cells as Products ISBN: 9780792360759
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::367d0559b195a9660f1b42804cebd72b
https://doi.org/10.1007/0-306-46875-1_72
https://doi.org/10.1007/0-306-46875-1_72
Publikováno v:
Biotechnology and bioengineering. 65(2)
This study correlates the fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where
Publikováno v:
Transgenic Organisms and Biosafety ISBN: 9783540610779
Chinese Hamster Ovary (CHO) cells are a popular mammalian host for the production of complex proteins for clinical applications. Today, more than 60% of candidate proteins for therapeutic and prophylactic purposes are being produced in mammalian cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3a2bfbc8e32256b0781953641034c6d3
https://doi.org/10.1007/978-3-642-61460-6_28
https://doi.org/10.1007/978-3-642-61460-6_28
Autor:
C J, Refino, N F, Paoni, B A, Keyt, C S, Pater, J M, Badillo, F M, Wurm, J, Ogez, W F, Bennett
Publikováno v:
Thrombosis and haemostasis. 70(2)
In the accompanying paper, we reported that the properties of decreased plasma clearance rate, increased fibrin specificity, and resistance to inactivation by PAI-1 could be effectively combined in the t-PA variant T103N, KHRR 296-299 AAAA. In the cu
Autor:
N F, Paoni, B A, Keyt, C J, Refino, A M, Chow, H V, Nguyen, L T, Berleau, J, Badillo, L C, Peña, K, Brady, F M, Wurm
Publikováno v:
Thrombosis and haemostasis. 70(2)
Site directed mutagenesis was used to construct a t-PA variant that contains an additional glycosylation site in the first kringle domain (T103N) combined with a tetra-alanine substitution in the protease domain (KHRR 296-299 AAAA). This combination
Publikováno v:
Developments in biological standardization. 76
Fluorescence in situ hybridization (FISH, 15) and high density, non-selective long term perfusion culture were used to study aspects of genetic stability and productivity in recombinant CHO cells. We analysed the distribution and structure of recombi
Autor:
W F, Bennett, N F, Paoni, B A, Keyt, D, Botstein, A J, Jones, L, Presta, F M, Wurm, M J, Zoller
Publikováno v:
The Journal of biological chemistry. 266(8)
Sixty-four variants of human tissue-type plasminogen activator (tPA) were produced using recombinant DNA techniques. Charged residues were converted to alanine in clusters of from one to four changes per variant; these clusters spanned all the domain
Publikováno v:
Developments in biological standardization. 75
The presence of budding C-type and intracytoplasmic A-type particles in Chinese hamster ovary (CHO) cells is well documented. However, extensive screening has failed to detect any evidence of infectivity. To investigate the origin and expression of t