Optimization of adenosine deaminase assay by response surface methodology

Autor: Francesca Canalias, F J Gella, Alexandre Bota

Publikováno v: Clinica Chimica Acta. 290:145-157

The effect of four variables (adenosine, glutamate dehydrogenase, phosphate buffer, and pH) on the measured catalytic concentration of adenosine deaminase (EC 3.5.4.4) was studied by Response Surface Methodology (RSM). This multivariate methodology o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1deefb28ac94e36d29a59265162d5959
https://doi.org/10.1016/s0009-8981(99)00165-5

Brain pyruvate oxidation in experimental thiamin-deficiency encephalopathy

Autor: Jaume Coll-Cantí, Jorge Beleta, P. Munujos, F González-Sastre, F J Gella

Publikováno v: Clinica Chimica Acta. 255:13-25

Pyrithiamine-induced thiamin deficiency has been used in rat as an experimental form of Wernicke-Korsakoff encephalopathy, a disease associated with chronic alcoholism. Although the main etiological factor is known to be the lack of thiamin, the bioc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ee51fc8c9ec8d930ca79870f1a2d80f
https://doi.org/10.1016/0009-8981(96)06385-1

Assay of Succinate Dehydrogenase Activity by a Colorimetric-Continuous Method Using Iodonitrotetrazolium Chloride as Electron Acceptor

Autor: F J Gella, F González-Sastre, P. Munujos, Jaume Coll-Cantí

Publikováno v: Analytical Biochemistry. 212:506-509

A spectrophotometric assay method for determining succinate dehydrogenase activity is described in which iodonitrotetrazolium chloride is used as a final electron acceptor. The enzyme activity is determined by measuring the formation of formazan due

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91f4e404d09505c4f50ac9f80a80e5a9
https://doi.org/10.1006/abio.1993.1360

Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608

Autor: C. Profilis, Xavier Fuentes-Arderiu, Maribel Sánchez, E. Cusó, Mauro Panteghini, K. Hallermayer, Mogens Hørder, Ferruccio Ceriotti, F J Gella, Francesca Canalias, G. Schumann

Publikováno v: Sánchez, M, Gella, F-J, Profilis, C, Ceriotti, F, Cusó, E, Fuentes-Arderiu, X, Hallermayer, K, Hørder, M, Panteghini, M, Schumann, G & Canalias, F 2001, ' Certification of the Mass Concentration of Creatine Kinase Isoenzyme 2 (CK-MB) in the Reference Material BCR 608 ', Clinical Chemistry and Laboratory Medicine, vol. 39, no. 9, pp. 858-865 . https://doi.org/10.1515/CCLM.2001.142

We describe the certification of a mass concentration value in the already prepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2 was purified from human heart. The purified enzyme was diluted in order to measure its protein conce

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::221b620fc7b50e3fcbe35a51f87214d3
https://portal.findresearcher.sdu.dk/da/publications/e47cf668-79dc-4ce1-9370-330cb79756dd

Reference materials in clinical enzymology: preparation, requirements and practical interests

Autor: D W, Moss, I, Maire, D H, Calam, R E, Gaines Das, J M, Lessinger, F J, Gella, G, Férard

Publikováno v: Annales de biologie clinique. 52(3)

Five enzyme materials (gamma-glutamyltransferase, alkaline phosphatase, creatine kinase, alanine aminotransferase and prostatic acid phosphatase) are currently certified using a reference method. Furthermore, feasibility studies have been performed f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c174e1d7e1d9ffacfb964101b01172f5
https://pubmed.ncbi.nlm.nih.gov/7998675