Zobrazeno 1 - 10
of 64
pro vyhledávání: '"F, Whittier"'
Autor:
Robert F. Whittier, Machiko Hatsuda
Publikováno v:
Juntendo Medical Journal. 64:96-97
Autor:
Hidenori Yamanaka, Enrique Carredano, Yukio Hasegawa, Robert F. Whittier, Masanori Gotoh, Yuko Kaneda, Hiroyuki Sota, Yasuro Shinohara
Publikováno v:
Journal of Biological Chemistry. 277:16928-16935
Despite very similar tertiary structures based upon a common framework, legume lectins exhibit an amazing variety of sugar binding specificities. While most of these lectins recognize rather discrete sugar linkages, Phaseolus vulgaris erythroagglutin
Autor:
Yasuro Shinohara, Robert F. Whittier, Yoshio Okahata, Yukio Hasegawa, Hiroshi Yoshimine, Hiroyuki Sota, Masanori Gotoh
Publikováno v:
Anal. Chem.. 74:3592-3598
Despite high theoretical sensitivity, low-cost manufacture, and compactness potentially amenable to lab-on-a-chip use, practical hurdles have stymied the application of the quartz crystal microbalance (QCM) for aqueous applications such as detection
Autor:
Daisuke Shibata, Norihiro Mitsukawa, Motoaki Seki, Yao Guang Liu, Robert F. Whittier, Minami Matsui, Teruaki Taji, Takuya Ito, Nobuaki Hayashida, Kazuo Shinozaki, Toyoaki Anai, Tomohiko Tsuge
Publikováno v:
Gene. 225:31-38
Large insert capacity, clone stability and convenient propagation in Escherichia coli have made bacterial artificial chromosome and phage P1 vector-based libraries the first choice for large-scale sequencing projects, and these libraries have also pr
Publikováno v:
The Plant Journal. 8:457-463
Thermal asymmetric interlaced (TAIL-) PCR is an efficient technique for amplifying insert ends from yeast artificial chromosome (YAC) and P1 clones. Highly specific amplification is achieved without resort to complex manipulations before or after PCR
Autor:
Hiroo Fukuda, Atsushi Komamine, Robert F. Whittier, Takashi Toya, Ryoichi Kawahara, Shigeru Sato
Publikováno v:
Plant Molecular Biology. 28:39-46
We report the first successful isolation by subtractive hybridization of a gene expressed specifically during somatic embryogenesis. Embryogenic cell clusters, 32-50 microns in diameter, were isolated by sieving and density-gradient centrifugation. T
Publikováno v:
The Plant Journal. 7:351-358
Summary Using improved techniques, a representative P1 library of Arabidopsis was constructed and characterized. Megabase genomic DNA was prepared from nuclei and partially digested with Sau3AI. DNA fragments of 75–100 kb were selected by size frac
Autor:
Robert F. Whittier, Yao-Guang Liu
Publikováno v:
Genomics. 25:674-681
Isolation of DNA segments adjacent to known sequences is a tedious task in genome-related research. We have developed an efficient PCR strategy that overcomes the shortcomings of existing methods and can be automated. This strategy, thermal asymmetri
Publikováno v:
Bioscience, biotechnology, and biochemistry. 62(1)
A genomic clone containing part of the coding region and upstream sequences of a phosphoenolpyruvate carboxylase (PEPC) gene was isolated from a soybean genomic library. The first intron of this gene is located in the 5′ untranslated region. This i
Publikováno v:
The Plant journal : for cell and molecular biology. 10(4)
A new collection of 129 Arabidopsis thaliana RFLP markers has been established based upon DNA fragments cloned in the pUC119 plasmid vector and insert end sequences of P1 clones. Dominant/null alleles affecting low-copy number sequences account for n